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Figure 3: Immunohistochemical analysis of nuclear factor-kappa B in heart tissue of different group animals (Scale bar-100 μm): (IHC, ×40) (a) Group I/Normal control (sterile phosphate buffer saline as Zym vehicle), (b) Group II/high-fat diet (30 days) and Zym (80 mg/kg, single intraperitoneal injection at day 8th). The Fiji (Image J) software was employed for the semi-quantification of the expression of proteins. Data in the graph are expressed as mean ± standard error of mean (n = 6 animals per group). Significance was determined by unpaired t-test ***P < 0.001 (Group II vs. I)

Figure 3: Immunohistochemical analysis of nuclear factor-kappa B in heart tissue of different group animals (Scale bar-100 μm): (IHC, ×40) (a) Group I/Normal control (sterile phosphate buffer saline as Zym vehicle), (b) Group II/high-fat diet (30 days) and Zym (80 mg/kg, single intraperitoneal injection at day 8<sup>th</sup>). The Fiji (Image J) software was employed for the semi-quantification of the expression of proteins. Data in the graph are expressed as mean ± standard error of mean (<i>n</i> = 6 animals per group). Significance was determined by unpaired <i>t</i>-test ***<i>P</i> < 0.001 (Group II vs. I)