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Figure 5: Regulation of Carthamus tinctorius L. on apoptosis of brain tissues of rats after cerebral ischemia. (A) Transferase-mediated dUTP nick-end labeling assay for apoptosis; (B) Western blotting for apoptosis-related factor levels including B-cell lymphoma-2, Bax, and Caspase3; (C) Reverse transcription polymerase chain reaction for messenger RNA levels of B-cell lymphoma-2, Bax and Caspase3. Nimodipine of 7.53 mg/kg/d was used as positive drug. Data are presented as means ± standard deviation (n = 6).#P < 0.05,##P < 0.01, versus Sham; *P < 0.05, **P < 0.01, versus model

Figure 5: Regulation of <i>Carthamus tinctorius</i> L. on apoptosis of brain tissues of rats after cerebral ischemia. (A) Transferase-mediated dUTP nick-end labeling assay for apoptosis; (B) Western blotting for apoptosis-related factor levels including B-cell lymphoma-2, Bax, and Caspase3; (C) Reverse transcription polymerase chain reaction for messenger RNA levels of B-cell lymphoma-2, Bax and Caspase3. Nimodipine of 7.53 mg/kg/d was used as positive drug. Data are presented as means ± standard deviation (<i>n</i> = 6).<sup>#</sup><i>P </i>< 0.05,<sup>##</sup><i>P </i>< 0.01, versus Sham; *<i>P</i> < 0.05, **<i>P</i> < 0.01, versus model