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Figure 2: Spot Test Assay: Incubated various concentrations of selected formulations with 0.125% collagenase for 10 min. A drop of 3μl was carefully placed on a piece of X-ray film, incubated for 10 min and washed in tap water. Clearance of gelatin coating of X-ray film indicated enzyme activity. (a) Ashwagandharishta showing complete inhibition of collagenase at 75 mg/ml, (b) Balarishta showing inhibition at 50mg/ml, and (c) Dashmoolarishta showing partial and complete inhibition at 39 and 78mg/ml, respectively. (d) Triphala extract showing partial and complete inhibition at 10 and 20mg/ml

Figure 2: Spot Test Assay: Incubated various concentrations of selected formulations with 0.125% collagenase for 10 min. A drop of 3μl was carefully placed on a piece of X-ray film, incubated for 10 min and washed in tap water. Clearance of gelatin coating of X-ray film indicated enzyme activity. (a) <i>Ashwagandharishta</i> showing complete inhibition of collagenase at 75 mg/ml, (b) <i>Balarishta</i> showing inhibition at 50mg/ml, and (c) <i>Dashmoolarishta</i> showing partial and complete inhibition at 39 and 78mg/ml, respectively. (d) <i>Triphala</i> extract showing partial and complete inhibition at 10 and 20mg/ml