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Figure 4: HCT-8 colorectal cancer cells treated with AB venom for 24 h and 48 h (a) Bright field untreated. (b) Bright field 24 h treated. (c) Bright field 48 h treated. (d) 4',6-diamidino-2-phenylindole stained untreated. (e) 4',6-diamidino-2-phenylindole stained 24 h treated (f). 4',6-diamidino-2-phenylindole stained 48 h treated. (g) Propidium iodide stained untreated. (h) Propidium iodide stained 24 h treated. (i) Propidium iodide stained 48 h treated. (j) Percentage of normal, apoptotic, and necrotic cells at 24 h and 48 h in control and AB venom-treated HCT-8 cells. Arrows are indicating the apoptotic cells. Statistically significant (P ≤ 0.05)

Figure 4: HCT-8 colorectal cancer cells treated with AB venom for 24 h and 48 h (a) Bright field untreated. (b) Bright field 24 h treated. (c) Bright field 48 h treated. (d) 4',6-diamidino-2-phenylindole stained untreated. (e) 4',6-diamidino-2-phenylindole stained 24 h treated (f). 4',6-diamidino-2-phenylindole stained 48 h treated. (g) Propidium iodide stained untreated. (h) Propidium iodide stained 24 h treated. (i) Propidium iodide stained 48 h treated. (j) Percentage of normal, apoptotic, and necrotic cells at 24 h and 48 h in control and AB venom-treated HCT-8 cells. Arrows are indicating the apoptotic cells. Statistically significant (<i>P</i> ≤ 0.05)