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Figure 2: Chemical genetic effect of Sargassum wightii in zebrafi sh embryos. (a) has mutation (curved body axis) and heg mutation (tubular heart formation) at 3 dpf. (b) Black arrow showing has mutation and white arrows shows dou yan mutation (reduction of eye size) at 4 dpf. (c) 3 dpf embryonic heart with pericardial bulging. (d) heg mutation, has mutation (black arrow), mouth protrudation and eye mutation at 4 dpf. (e) Magnifi ed view of the 2c shows the tubular heart (white arrow) as single-cell – layered myocardium at 4 dpf. Black arrow shows the mouth protrudation in the 4 dpf embryos. (f) Control embryo at 3 dpf

Figure 2: Chemical genetic effect of <i>Sargassum wightii</i> in zebrafi sh embryos. (a) <i>has mutation (curved body axis)</i> and <i>heg</i> mutation (tubular heart formation) at 3 dpf. (b) Black arrow showing <i>has</i> mutation and white arrows shows <i>dou yan</i> mutation (reduction of eye size) at 4 dpf. (c) 3 dpf embryonic heart with pericardial bulging. (d) <i>heg</i> mutation, <i>has</i> mutation (black arrow), mouth protrudation and eye mutation at 4 dpf. (e) Magnifi ed view of the 2c shows the tubular heart (white arrow) as single-cell – layered myocardium at 4 dpf. Black arrow shows the mouth protrudation in the 4 dpf embryos. (f) Control embryo at 3 dpf