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Figure 2: Effect of rosiglitazone in LanthaScreen™ TR-FRET PPARgamma coactivator assay in the agonist mode. Mean ± SEM (n = 4). The reaction mixture contained 5 nM GST-tagged PPAR gamma receptor, 125 nM of coregulator peptide, 5 nM of TB-anti-GST-tagged antibody, 5 mM DTT (dithiothreitol), and varying concentrations of rosiglitazone (1000-0.0056 nM) Following 1-h incubation in the dark, TR-FRET measurements were made in the PHERAstar multilabel plate reader

Figure 2: Effect of rosiglitazone in LanthaScreen™ TR-FRET PPARgamma coactivator assay in the agonist mode. Mean ± SEM (n = 4). The reaction mixture contained 5 nM GST-tagged PPAR gamma receptor, 125 nM of coregulator peptide, 5 nM of TB-anti-GST-tagged antibody, 5 mM DTT (dithiothreitol), and varying concentrations of rosiglitazone (1000-0.0056 nM) Following 1-h incubation in the dark, TR-FRET measurements were made in the PHERAstar multilabel plate reader