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Figure 1: Principle of the nuclear receptor (NR) agonist-dependent coactivator peptide recruitment assay: Tb-anti-GST antibody indirectly labels the nuclear receptor by binding to the GST tag. Binding of the agonist to the NR causes a conformational change that results in an increase in the affinity of the NR for a coactivator peptide. The close proximity of the fl uorescently labeled coactivator peptide to the terbium-labeled antibody causes an increase in the TR-FRET signal. (Reproduced with permission from Invitrogen Corporation, USA)

Figure 1: Principle of the nuclear receptor (NR) agonist-dependent coactivator peptide recruitment assay: Tb-anti-GST antibody indirectly labels the nuclear receptor by binding to the GST tag. Binding of the agonist to the NR causes a conformational change that results in an increase in the affinity of the NR for a coactivator peptide. The close proximity of the fl uorescently labeled coactivator peptide to the terbium-labeled antibody
causes an increase in the TR-FRET signal. (Reproduced with permission from Invitrogen Corporation, USA)