Development of a psoriatic-like skin inflammation rat model using imiquimod as an inducing agent,Komal M Parmar1, Chetan S Jagtap1, Nitin T Katare2, Mahaveer Dhobi3, Satyendra K Prasad1, : Indian Journal of Pharmacology

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RESEARCH ARTICLE	[Download PDF]

Year : 2021 \| Volume : 53 \| Issue : 2 \| Page : 125--131

Development of a psoriatic-like skin inflammation rat model using imiquimod as an inducing agent

Komal M Parmar¹, Chetan S Jagtap¹, Nitin T Katare², Mahaveer Dhobi³, Satyendra K Prasad¹,
¹ Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur, Maharashtra, India
² Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur, Maharashtra, India; Department of Chemistry, Western Kentucky University, Bowling Green, KY, United States
³ Department of Pharmacognosy and Phytochemistry, Delhi Pharmaceutical Sciences and Research University, New Delhi, India

Correspondence Address:
Dr. Satyendra K Prasad
Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur, Maharashtra
India

Abstract

Objective: The present investigation was undertaken to develop a psoriatic-like skin inflammation rat model using imiquimod (IMQ) as an inducing agent. Materials and Methods: The hairs of the back dorsal portion of the Wistar rats were removed and 80, 100, and 120 mg of IMQ cream (5% w/w) for 10 consecutive days was applied to different groups of rats. Further, psoriasis area severity index was used for calculating the psoriatic score, which included scoring of erythema, scaling, and thickening. Various biochemical parameters, pro-inflammatory cytokines, vascular endothelial growth factor (VEGF), and histopathological examination were also performed. Results: The results demonstrated signs of erythema, scaling, and thickening on group applied with 120 mg and 100 mg of IMQ along with ear thickening. Biochemical evaluation revealed a significant increase in the granulation tissue weight followed by significant decrease in the levels of collagen and hexosamine. The antioxidant parameters superoxide dismutase and catalase were found to decline, while nitric oxide and lipid peroxidation were significantly elevated in skin lesions, also supported by increased pro-inflammatory cytokines expression, i.e., interleukin (IL)-1 β, IL-6, IL-17, tumor necrosis factor-α, and VEGF. Histopathological studies revealed a disturbed natural structure along with increased epidermal proliferation, abnormal differentiation with increased number of keratinocytes in the psoriatic skin tissue. Conclusion: From the overall study, we have successfully developed a psoriatic-like skin inflammation rat model for the first time on Wistar strain using IMQ as an inducing agent.

How to cite this article:
Parmar KM, Jagtap CS, Katare NT, Dhobi M, Prasad SK. Development of a psoriatic-like skin inflammation rat model using imiquimod as an inducing agent.Indian J Pharmacol 2021;53:125-131

How to cite this URL:
Parmar KM, Jagtap CS, Katare NT, Dhobi M, Prasad SK. Development of a psoriatic-like skin inflammation rat model using imiquimod as an inducing agent. Indian J Pharmacol [serial online] 2021 [cited 2023 Jun 9 ];53:125-131
Available from: https://www.ijp-online.com/text.asp?2021/53/2/125/316953

Full Text

Introduction

Psoriasis is a disorder that occurs due to immune imbalance, which has already been confirmed by a number of researchers since the past two decades. It is considered to be a life-long chronic immune-mediated inflammatory disease, which is identified by patches of thick skin appearing red and is covered with silvery scales that ranges from few patches to almost the entire body surface.[1],[2] It is estimated that more than 7 million Americans and 2%–4% of the population worldwide are affected from psoriasis.[3]

In investigations associated to psoriasis, there are very few available applicable animal models so far. Epidermal hyperproliferation, papillomatosis, altered differentiation, presence of inflammatory cells, and abnormal vascularity are the ideal features of a psoriatic model. Histological characteristics related to psoriasis typically include epidermal hyperplasia along with elongated rete ridges, hypogranulosis, parakeratosis, dermis and epidermis leukocytic infiltration.[4] At present, there are several animal models which were quite similar to psoriasis; however, clinical and pathological resemblance was observed in very few models. Many genetically engineered models which includes transgenic/knockout or induced type model such as xenotransplantation murine psoriasis models have been developed up to date.[5] Xenograft model is one of the psoriatic mouse models, where psoriatic skin (human) is transplanted on immunodeficient mice that closely resemblance of humans psoriasis with respect to its genetic, phenotypic, and immunological characteristics. However, it has several drawbacks such as it is expensive, requires specialized laboratory skills, and time consuming.[6]

Ideal psoriatic animal models must have clinical hallmarks and must represent particular histomorphological patterns of psoriasis, easily reproducible, inexpensive, and ethical.[7] Imiquimod (IMQ), a well-known immune activator, is said to be a ligand for toll-like receptor 7 (TLR7) and TLR8 and has also recently been reported for its psoriasis-like inflammatory effects. The immunemodulatory action of IMQ in inducing psoriasis is observed due to TLR7stimulation on plasmacytoid dendritic cells along with upregulation of Type I interferon pathway. IMQ-mouse model is said to be a suitable experimental model for psoriasis-related studies that presents a typical psoriatic phenotype, which interfere with adenosine receptor signaling pathway and augment inflammation.[8] A study depicted the role of interleukin (IL)-23/IL-17 axis, in IMQ-induced skin inflammation in mice, which was also confirmed through histopathological examination showing similar characteristics to psoriasis. Therefore, IMQ is considered to be very preferable and convenient model to study the mechanistic evaluations related to acute inflammatory response in animal as well as human.[8] In the present study, we have tried to develop a suitable rat model for psoriatic-like skin inflammation using IMQ as an inducing agent, which may prove to be quite important for researchers working in the field of psoriasis.

Materials and Methods

Experimental animals

Healthy rats of Wistar strain (150–200 g) were obtained from the Central Animal House of Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur, Maharashtra, India, having Reg. No.: 92/1999/CPCSEA (dated: April 28, 1999). The animals were maintained and acclimatized for a period of 7 days before experiment by keeping them in standard environmental conditions, namely, 12 h light/dark cycle, ambient temperature (25°C ± 1°C), and relative humidity (45%–55%). The rats were fed with commercially available rat feed and water ad libitum. The protocols involved in the present investigation were approved from Central Animal Ethical Committee of Rashtrasant Tukadoji Maharaj Nagpur University (Letter No.: IAEC/UDPS/2014/38 dated December 22, 2014) and were carried out as per the standard guidelines of National Institutes of Health Guide for Care and Use of Laboratory Animals (Publication No. 85-23, revised 1985).

Imiquimod-induced psoriatic rat model

Rats of 12–14-week-old were adopted for this study and their hairs were removed by shaving dorsal portion of each rat, while depilatory cream (Veet, Reckitt Benckiser Pvt. Ltd., India) was used to remove the remaining hairs. The rats were divided into four groups (six animals each), where Group I was served as normal control and was applied with vehicle cream (Vaseline). Group II, III, and IV were applied with IMQ 5% w/w cream (Glenmark Pharmaceuticals Pvt. Ltd, Nasik, India) with a daily quantity of 80, 100, and 120 mg, respectively, on the back skin and 10 mg was applied to the right ear for 10 consecutive days.[9]

Scoring severity of skin inflammation and ear thickness

The severity of skin inflammation was measured by calculating the psoriasis area severity index (PASI) where scaling, erythema, and thickening were independently scored from 0 to 4. In this scale, 0 corresponds to none; 1 corresponds to slight; 2 corresponds to moderate; 3 corresponds to marked, and 4 corresponds to very marked.[10] The thickness observed in the right ear of the rats was also measured with the help of a Vernier calliper and was also referred as a measure of skin inflammation.

Biochemical analysis

Rats were euthanized by administering thiopental sodium (65 mg/kg, i.p.) at the end of 10th postoperative day and the psoriatic skin tissues of both dorsal and ear portion were removed and immediately rinsed using tyrode solution. Some portion of the tissues was dried, weighed followed by treatment with 6N HCl on water bath for 24 h to undergo hydrolysis, and the obtained hydrolysate was further subjected to estimations of hexosamine, hydroxyproline, and hyaluronic acid following standard protocol.[11],[12],[13] Some part of the collected tissue samples was homogenized in phosphate buffer and the supernatant so obtained was used for determination of nitric oxide (NO) adopting the standard procedure given by Green et al.[14] The tissue samples were also subjected to determination of total DNA[15] and protein content.[16] Further, the study also includes antioxidant evaluations, which included lipid peroxidation (LPO), catalase (CAT), and superoxide dismutase (SOD).[17]

Estimation of cytokines and vascular endothelial growth factor

Enzyme-linked immunosorbent assay (ELISA) method was adopted for the estimations of pro-inflammatory cytokines, i.e., IL-1 β, IL-6, IL-17, tumor necrosis factor-α (TNF-α) (Komabiotech, Korea), and vascular endothelial growth factor (VEGF) (RayBiotech) with the help of commercially available ELISA kits on tissue homogenate. The procedure for the following estimation was carried out as per the instructions given by the manufacture.

Histopathological examination

The sample tissues obtained on 10th day of the experiment were immediately fixed in formalin (10%) followed by dehydration in acetone, which were further embedded in paraffin wax and were processed for taking sections using microtome (4 μm thicknesses). The staining of the tissues sections was done using hematoxylin-eosin (1:1) and were subjected to histological examination with the help of a digital Trinocular microscope (Leica DM 2000).

Statistical analysis

All the experimental results are expressed as mean ± standard error of the mean (n = 6), followed by one-way analysis of variance. Newman–Keuls multiple comparisons test was used for assessing the statistical difference between different groups. Graph Pad Prism, version 5 software (San Diego, CA, USA), was adopted for statistical analysis and P < 0.05 was set as significant level.

Results

Structural features of imiquimod -induced skin inflammation

After 6 days of IMQ application, rats from the group applied with 120 mg and 100 mg of IMQ, displayed the signs of erythema, scaling, and thickening, whereas application of 80 mg of IMQ did not show any sign of inflammation [Figure 1] and [Figure 2]. The study also involved the measurement of skin inflammation, on right ear as indicated by ear thickness in rats [Figure 3]. A significant increase in the ear thickness was observed on daily application of IMQ to the right ear, which was quite measurable from day 6 onward in the groups treated with 120 and 100 mg of IMQ.{Figure 1}{Figure 2}{Figure 3}

Biochemical estimations

The results of the biochemical analysis performed in the present study are represented in [Table 1]. The overall results showed a significant increase in the weight of granulation tissue, while a significant (P < 0.05) decline in total protein and DNA content in granulation tissues of IMQ induced rats was observed, where 120 mg was found to be more prominent as compared to 80 mg, 100 mg, and normal one. The IMQ-induced rats also showed a significant (P < 0.05) decline in the levels of hydroxyproline and hexosamine with a concomitant increase in the levels of hyaluronic acid as compared to normal rats justifying the induction of psoriasis-like inflammation. The results also demonstrated an alteration in the antioxidant status of the IMQ applied rats, where a significant (P < 0.05) increase in the levels of NO and LPO was observed, while a significant (P < 0.05). decrease in the levels of SOD and CAT was noted.{Table 1}

Effect on pro-inflammatory cytokines and vascular endothelial growth factor

[Figure 4] represents the impact of the IMQ application on pro-inflammatory cytokines and VEGF on skins of the rats. The results depicted a significant increase in the levels of all the tested pro-inflammatory cytokines in the IMQ-induced rats at all concentrations, where significant effect was observed at 100 and 120 mg of IMQ application. Further, the VEGF levels were found to be significantly increased in the IMQ-treated groups at 120 mg.{Figure 4}

Histopathological studies

[Figure 5] and [Figure 6] represent the histopathological view of the psoriatic skin lesions (dorsal portion and ear) of the rats administered with IMQ, where epidermal thickening with increased epidermal proliferation and abnormal differentiation were observed along with increased hyperkeratosis with basal cell layer comprising large numbers of keratinocytes.{Figure 5}{Figure 6}

Discussion

Psoriasis is characterized mainly by hyperplasia of the epidermis, thickening of the cornified layer, and infiltration of inflammatory cell into dermis/epidermis followed by erythematous plaque formation.[18] Many investigations are underway to develop a suitable and authentic animal model for psoriasis showing pathologically similar characteristics with that of human. IMQ-induced psoriatic model involves a single synthetic innate, which is an Ag receptor ligand[8] and thus, in immunological terms, it may be considered as clean, as it does not require any adjuvants. In the present study, we have selected the Wistar strain of the rats as it is easily available at a very low cost and are also very adaptive in nature as compared to the previously investigated genetically modified[8] mice strains, which are expensive and also requires special attention and care. The present study demonstrated that application of IMQ for 10 successive days of experimental period showed rat's skin closely resembling human psoriasis in terms of skin thickening, scaling epidermal alterations, and erythema. Application of IMQ at 120 and 100 mg showed increased thickening of epidermal layer, erythema, and hyperkeratosis, which reflected in abnormal differentiation of keratinocyte, as a result of increased proliferation and increased expression of cytokine. The study also confirmed ear inflammation, which was evidenced through ear weight and ear thickness on application of IMQ. Further, there was also a significant increase in PASI at 100 and 120 mg of IMQ application; however, at 80 mg of IMQ application; there was no significant increase in PASI.

Collagen is considered to be an important building block for the skin as it is a very important component of connective tissue that maintains the structure and elasticity of skin. The results demonstrated a decrease in the level of collagen (hydroxyproline), whereas an increase in the level of hyaluronic acid was observed on IMQ application. Hyaluronic acid plays a key role in nutrient exchange, water retention, cell mobilization, and cell differentiation.[19] Accumulation of collagen increases the hexosamine levels as hexosamine helps in electrostatic bonding by providing collagen sites.[20] Thus, it may be presumed that application of IMQ to rats resulted in the development of psoriatic-like skin lesions and deformation of normal skin structure, which may be due to the decreased levels of collagen and hexosamine.

Application of IMQ significantly increased the expression of pro-inflammatory cytokines, namely, TNF-α, IL-1 β, IL-6, and IL-17 which co-operatively stimulated the keratinocytes for producing a variety of inflammatory mediators and growth factors that ultimately resulted in fuelling the psoriatic inflammation vicious cycle.[21] An imbalance in antioxidant system is also one of the important characteristic features for pathogenesis of psoriasis.[22] LPO is also said to be one of the major characteristic features in the advanced stage of psoriasis.[23] Therefore, our observation also revealed an increase in the level of LPO followed by decline in the essential antioxidants SOD and CAT, confirming an abnormal antioxidant status during psoriasis. Our observation also showed a significant elevation in the NO level on IMQ application. NO in a certain amount has been reported to participates in normal healing;[24] however, high concentration of NO in relation with elevated expression of inducible NO synthase (iNOS) in psoriasis plaques, and more specifically in keratinocytes,[25],[26] are mainly concerned with the psoriasis pathogenesis. Therefore, treatment of psoriasis also focuses on reducing the level of NO, which ultimately will reduce the expression of iNOS.

Literature has revealed that overexpression of VEGF results to increased dermis vascularity, which is said to be an important characteristics features of psoriatic plaques.[27] It has been reported that VEGF circulating levels is generally higher in active psoriasis and lowers down during disease remission.[28] Since VEGF plays a significant role in the pathogenesis of the psoriasis, it is considered to be a promising biomarker in understanding the progression of these diseases. The data obtained from the results did showed the increased expression of VEGF on application of IMQ at 100 and 120 mg, which confirmed the pathogenesis of psoriasis. The results from histopathological examination also revealed an increased epidermal layer thickening, hyperkeratosis, and rete-like ridges elongation at 100 and 120 mg of IMQ application. As compared to the previous investigations, the main salient features [Table 2] of our study involve the successful use of a very common strain of rat, which is more cheaper and easier to handle as compared to highly expensive genetically modified mice strain. Further, the availability of large surface area in rats could also prove beneficial in the measurement of severity of inflammation and other analysis. Like previous studies, our study also justified the role of ILs[8] axis in induction of psoriasis.{Table 2}

Conclusion

Thus, from the overall study, we have successfully developed a psoriatic-like skin inflammation rat model for the first time using Wistar strain. The study also confirmed that IMQ at a dose range from 100 to 120 mg, if applied for 10 successive days could develop psoriasis-like skin inflammation on the Wistar rats. Furthermore, this model could prove very cheaper and suitable for a rapid faster in vivo screening of antipsoriatic drugs under investigation.

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.

References

1	Sabat R, Philipp S, Höflich C, Kreutzer S, Wallace E, Asadullah K, et al. Immunopathogenesis of psoriasis. Exp Dermatol 2007;16:779-98.
2	Parisi R, Symmons DP, Griffiths CE, Ashcroft DM, Identification and Management of Psoriasis and Associated ComorbidiTy (IMPACT) Project Team. Global epidemiology of psoriasis: A systematic review of incidence and prevalence. J Invest Dermatol 2013;133:377-85.
3	Schleicher SM. Psoriasis: Pathogenesis, assessment, and therapeutic update. Clin Podiatr Med Surg 2016;33:355-66.
4	Krueger JG, Bowcock A. Psoriasis pathophysiology: Current concepts of pathogenesis. Ann Rheum Dis 2005;64 Suppl 2:i30-6.
5	Danilenko DM. Review paper: Preclinical models of psoriasis. Vet Pathol 2008;45:563-75.
6	Nickoloff BJ, Wrone-Smith T. Injection of pre-psoriatic skin with CD4+ T cells induces psoriasis. Am J Pathol 1999;155:145-58.
7	Zollner TM, Renz H, Igney FH, Asadullah K. Animal models of T-cell-mediated skin diseases. Bioessays 2004;26:693-6.
8	van der Fits L, Mourits S, Voerman JS, Kant M, Boon L, Laman JD, et al. Imiquimod-induced psoriasis-like skin inflammation in mice is mediated via the IL-23/IL-17 axis. J Immunol 2009;182:5836-45.
9	Parmar KM, Itankar PR, Joshi A, Prasad SK. Anti-psoriatic potential of Solanum xanthocarpum stem in imiquimod-induced psoriatic mice model. J Ethnopharmacol 2017;198:158-66.
10	Baek JO, Byamba D, Wu WH, Kim TG, Lee MG. Assessment of an imiquimod-induced psoriatic mouse model in relation to oxidative stress. Arch Dermatol Res 2012;304:699-706.
11	Neuman RE, Logan MA. The determination of collagen and elastin in tissue. J Biol Chem 1950;186:549-56.
12	Bitter T, Muir HM. A modified uronic acid carbazole reaction. Anal Biochem 1962;4:330-4.
13	Dische Z, Borenfreund E. A spectrophotometric method for the microdetermination of hexosamines. J Biol Chem 1950;184:517-22.
14	Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR. Analysis of nitrate, nitrite, and [15N] nitrate in biological fluids. Anal Biochem 1982;126:131-38.
15	Burton K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J 1956;62:315-23.
16	Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193:265-75.
17	Laloo D, Prasad SK, Krishnamurthy S, Hemalatha S. Gastroprotective activity of ethanolic root extract of Potentilla fulgens Wall. ex Hook. J Ethnopharmacol 2013;146:505-14.
18	Grine L, Dejager L, Libert C, Vandenbroucke RE. An inflammatory triangle in psoriasis: TNF, type I IFNs and IL-17. Cytokine Growth Factor Rev 2015;26:25-33.
19	Chen M, Gupta V, Anselmo AC, Muraski JA, Mitragotri S. Topical delivery of hyaluronic acid into skin using SPACE-peptide carriers. J Control Release 2014;173:67-74.
20	Pather N, Kramer B. Bulbine natalensis and Bulbine frutescens promote cutaneous wound healing. J Ethnopharmacol 2012;144:523-32.
21	Yoshiki R, Kabashima K, Honda T, Nakamizo S, Sawada Y, Sugita K, et al. IL-23 from Langerhans cells is required for the development of imiquimod-induced psoriasis-like dermatitis by induction of IL-17A-producing γδ T cells. J Invest Dermatol 2014;134:1912-21.
22	Zhou Q, Mrowietz U, Rostami-Yazdi M. Oxidative stress in the pathogenesis of psoriasis. Free Radic Biol Med 2009;47:891-905.
23	Yildirim M, Inaloz HS, Baysal V, Delibas N. The role of oxidants and antioxidants in psoriasis. J Eur Acad Dermatol Venereol 2013;17:34-6.
24	Efron T, Most D, Barbul A. Role of nitric oxide in wound healing. Curr opin Clin Nutr Metabol Care 2000;3:197-04.
25	Kolb-Bachofen V, Fehsel K, Michel G, Ruzicka T. Epidermal keratinocyte expression of inducible nitric oxide synthase in skin lesions of psoriasis vulgaris. Lancet 1994;344:139.
26	Ormerod AD, Weller R, Copeland P, Benjamin N, Ralston SH, Grabowksi P, et al. Detection of nitric oxide and nitric oxide synthases in psoriasis. Arch Dermatol Res 1998;290:3-8.
27	Griffiths CE, Barker JN. Pathogenesis and clinical features of psoriasis. Lancet 2007;370:263-71.
28	Flisiak I, Zaniewski P, Rogalska-Taranta M, Chodynicka B. Effect of psoriasis therapy on VEGF and its soluble receptors serum concentrations. J Eur Acad Dermatol Venereol 2012;26:302-7.