Palliative effects of lutein intervention in gamma-radiation-induced cellular damages in Swiss albino mice
Vidya Vasudeva1, Yogish Somayaji Tenkanidiyoor1, Vishakh Radhakrishna1, Pooja Shivappa1, Srikanth Patil Lakshman2, Ronald Fernandes3, Krishna Ananthapura Patali2
1 Research Scholar, Central Research Laboratory, K S Hegde Medical Academy, Nitte University, Mangalore, Karnataka, India
2 Department of Physiology, K S Hegde Medical Academy, Nitte University, Mangalore, Karnataka, India
3 Department of Biochemistry, Nitte Gulabi Shetty Memorial Institute of Pharmaceutical Sciences, Nitte University, Mangalore, Karnataka, India
Objectives: Radiation-induced hematological, biochemical, and cytogenetic damages to the normal cells are major concerns in the field of radiotherapy. The carotenoids and their derivatives have been the source of antioxidants with wide range of medicinal applications. The objective is to evaluate the protective effects of lutein, a carotenoid, against radiation-induced cellular and tissue damages.
Methods: Swiss albino mice were grouped into 5, 50, 250, and 500 mg/kg b.wt. of lutein treatment groups, a sham and vehicle control group. The groups were irradiated with a lethal dose of 10 Gy y'-radiation. The mortality was recorded for 30 days to optimize the protective dose against radiation. The mice were administered with the compound orally for 15 consecutive days and irradiated with a sublethal dose of 6Gy. The hematological changes in blood and antioxidant parameters were determined in liver, kidney homogenates, and hemolysate/serum. The hematological parameters were recorded using an automated cell counter. The antioxidants such as malondialdehyde (MDA), glutathione (GSH), superoxide dismutase, catalase, glutathione peroxidase and glutathione-S-transferase were spectrophotometrically determined.
Results: The red blood cell, white blood cell count, lymphocyte count, hemoglobin, platelet levels, and hematocrit value were found to be decreased in the irradiated groups. Lutein pretreatment maintains near-normal levels of these parameters indicating resistance/recovery from the radiation-induced damages. The antioxidant levels were found to be reduced in all the irradiated groups. However, lutein pretreatment (50 mg/kg b.wt.) has increased the catalase activity of hemolysate. Lutein pretreatment has reduced the MDA levels in hemolysate, when administered at doses of 5, 250, and 500 mg/kg b.wt. in comparison to its control.
Conclusion: The study demonstrates the radioprotective potential of lutein by maintaining the hematological and antioxidant homeostasis.
Krishna Ananthapura Patali
Department of Physiology, K S Hegde Medical Academy, Nitte University, Mangalore, Karnataka
|How to cite this article:|
Vasudeva V, Tenkanidiyoor YS, Radhakrishna V, Shivappa P, Lakshman SP, Fernandes R, Patali KA. Palliative effects of lutein intervention in gamma-radiation-induced cellular damages in Swiss albino mice.Indian J Pharmacol 2017;49:26-33
|How to cite this URL:|
Vasudeva V, Tenkanidiyoor YS, Radhakrishna V, Shivappa P, Lakshman SP, Fernandes R, Patali KA. Palliative effects of lutein intervention in gamma-radiation-induced cellular damages in Swiss albino mice. Indian J Pharmacol [serial online] 2017 [cited 2023 Dec 6 ];49:26-33
Available from: https://www.ijp-online.com/article.asp?issn=0253-7613;year=2017;volume=49;issue=1;spage=26;epage=33;aulast=Vasudeva;type=0