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Year : 2016  |  Volume : 48  |  Issue : 5  |  Page : 537--543

Scorpion (Androctonus bicolor) venom exhibits cytotoxicity and induces cell cycle arrest and apoptosis in breast and colorectal cancer cell lines

Abdulrahman K Al-Asmari1, Anvarbatcha Riyasdeen1, Rajamohamed Abbasmanthiri1, Mohammed Arshaduddin1, Fahad Ali Al-Harthi2 
1 Department of Research Center, Prince Sultan Military Medical City, Riyadh 11159, Saudi Arabia
2 Department of Dermatology, Prince Sultan Military Medical City, Riyadh 11159, Saudi Arabia

Correspondence Address:
Dr. Abdulrahman K Al-Asmari
Department of Research Center, Prince Sultan Military Medical City, Riyadh 11159
Saudi Arabia

Objectives: The defective apoptosis is believed to play a major role in the survival and proliferation of neoplastic cells. Hence, the induction of apoptosis in cancer cells is one of the targets for cancer treatment. Researchers are considering scorpion venom as a potent natural source for cancer treatment because it contains many bioactive compounds. The main objective of the current study is to evaluate the anticancer property of Androctonus bicolor scorpion venom on cancer cells. Materials and Methods: Scorpions were milked by electrical stimulation of telsons and lyophilized. The breast (MDA-MB-231) and colorectal (HCT-8) cancer cells were maintained in appropriate condition. The venom cytotoxicity was assessed by 3-(4,5-di-methylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay, and the cellular and nuclear changes were studied with propidium iodide and 4«SQ»,6-diamidino-2-phenylindole stain, respectively. The cell cycle arrest was examined using muse cell analyzer. Results: The A. bicolor venom exerted cytotoxic effects on MDA-MB-231 and HCT-8 cells in a dose- and duration-dependent manner and induced apoptotic cell death. The treatment with this venom arrests the cancer cells in G0/G1 phase of cell cycle. Conclusions: The venom selectively induces the rate of apoptosis in MDA-MB-231 and HCT-8 cells as reflected by morphological and cell cycle studies. To the best of our knowledge, this is the first scientific evidence demonstrating the induction of apoptosis and cell cycle arrest by A. bicolor scorpion venom.


How to cite this article:
Al-Asmari AK, Riyasdeen A, Abbasmanthiri R, Arshaduddin M, Al-Harthi FA. Scorpion (Androctonus bicolor) venom exhibits cytotoxicity and induces cell cycle arrest and apoptosis in breast and colorectal cancer cell lines.Indian J Pharmacol 2016;48:537-543


How to cite this URL:
Al-Asmari AK, Riyasdeen A, Abbasmanthiri R, Arshaduddin M, Al-Harthi FA. Scorpion (Androctonus bicolor) venom exhibits cytotoxicity and induces cell cycle arrest and apoptosis in breast and colorectal cancer cell lines. Indian J Pharmacol [serial online] 2016 [cited 2021 Jun 15 ];48:537-543
Available from: https://www.ijp-online.com/article.asp?issn=0253-7613;year=2016;volume=48;issue=5;spage=537;epage=543;aulast=Al-Asmari;type=0