Neuroprotective effect of Tinospora cordifolia ethanol extract on 6-hydroxy dopamine induced Parkinsonism
Jayasankar Kosaraju, Santhivardhan Chinni, Partha Deb Roy, Elango Kannan, A Shanish Antony, M. N. Satish Kumar
Department of Pharmacology, JSS College of Pharmacy, Udhagamadalam, Tamil Nadu, India
Objective: The present study investigates the neuroprotective activity of ethanol extract of Tinospora cordifolia aerial parts against 6-hydroxy dopamine (6-OHDA) lesion rat model of Parkinson«SQ»s disease (PD).
Materials and Methods: T. cordifolia ethanol extract (TCEE) was standardized with high performance thin layer chromatography using berberine. Experimental PD was induced by intracerebral injection of 6-OHDA (8 μg). Animals were divided into five groups: sham operated, negative control, positive control (levodopa 6 mg/kg) and two experimental groups (n = 6/group). Experimental groups received 200 and 400 mg/kg of TCEE once daily for 30 days by oral gavage. Biochemical parameters including dopamine level, oxidative stress, complex I activity and brain iron asymmetry ratio and locomotor activity including skeletal muscle co-ordination and degree of catatonia were assessed.
Results: TCEE exhibited significant neuroprotection by increasing the dopamine levels (1.96 ± 0.20 and 2.45 ± 0.40 ng/mg of protein) and complex I activity (77.14 ± 0.89 and 78.50 ± 0.96 nmol/min/mg of protein) at 200 and 400 mg/kg respectively when compared with negative control group. Iron asymmetry ratio was also significantly attenuated by TCEE at 200 (1.57 ± 0.18) and 400 mg/kg (1.11 ± 0.15) when compared with negative control group. Neuroprotection by TCEE was further supported by reduced oxidative stress and restored locomotor activity in treatment groups.
Conclusion: Results show that TCEE possess significant neuroprotection in 6-OHDA induced PD by protecting dopaminergic neurons and reducing the iron accumulation.
Department of Pharmacology, JSS College of Pharmacy, Udhagamadalam, Tamil Nadu
|How to cite this article:|
Kosaraju J, Chinni S, Roy PD, Kannan E, Antony A S, Kumar MS. Neuroprotective effect of Tinospora cordifolia ethanol extract on 6-hydroxy dopamine induced Parkinsonism.Indian J Pharmacol 2014;46:176-180
|How to cite this URL:|
Kosaraju J, Chinni S, Roy PD, Kannan E, Antony A S, Kumar MS. Neuroprotective effect of Tinospora cordifolia ethanol extract on 6-hydroxy dopamine induced Parkinsonism. Indian J Pharmacol [serial online] 2014 [cited 2021 Sep 17 ];46:176-180
Available from: https://www.ijp-online.com/article.asp?issn=0253-7613;year=2014;volume=46;issue=2;spage=176;epage=180;aulast=Kosaraju;type=0