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Year : 2006  |  Volume : 38  |  Issue : 4  |  Page : 301-


S Balian 
 Department of Pharmacognosy & Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, New Delhi, India

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S Balian
Department of Pharmacognosy & Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, New Delhi

How to cite this article:
Balian S. Reply.Indian J Pharmacol 2006;38:301-301

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Balian S. Reply. Indian J Pharmacol [serial online] 2006 [cited 2023 Sep 24 ];38:301-301
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The reader's observation that "10 spots were observed in the HPTLC fingerprint of methanolic leaf extract and 8 in that of leaf callus" is very correct. The higher amount can be justified from the peak area/peak height as obtained in different spots as well as from quantification of marker compounds of callus culture.

The main objective of the project was to produce silybin from in vitro cultures of Silybum marianum . The quantification of silybin was carried out using HPTLC method which has been reported.[1] In the report we have also mentioned and discussed about the higher activity of callus culture which is due to higher amount of secondary metabolites.

As indicated in the research letter the more number of spots in natural leaf as compared to that in vitro culture is mainly due to the absence of chlorophyll and other coloring matters in in vitro culture.


1Swati Balian, 2004, Tissue culture and Pharmacological studies on Silybum marianum Gaertn [M. Pharm. Thesis], submitted to Jamia Hamdard; New Delhi; 2004.