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Year : 1994  |  Volume : 26  |  Issue : 4  |  Page : 288--291

Determination of buprenorphine and its N-dealkylated metabolite in urine TLC densitometry

Jain Raka 
 

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Jain Raka


A densitometric method was developed for determination of buprenorphine and its N-dealkylated metabolite (norbuprenorphine) in urine. The procedure involves acid hydrolysis followed by liquid - liquid extraction. The extracted residue was subjected to thin - layer chromatography and separation was achieved in two different solvent systems, Quantitation was done by scanning the sprayed plate at 500 nm using densitometer. The detection limit of the method is 0.5 (g\ ml. The method is simple, rapid, reproducible and suitable for analysis of urine samples taken from abusers of buprenorphine.


How to cite this article:
Raka J. Determination of buprenorphine and its N-dealkylated metabolite in urine TLC densitometry.Indian J Pharmacol 1994;26:288-291


How to cite this URL:
Raka J. Determination of buprenorphine and its N-dealkylated metabolite in urine TLC densitometry. Indian J Pharmacol [serial online] 1994 [cited 2020 Oct 30 ];26:288-291
Available from: https://www.ijp-online.com/article.asp?issn=0253-7613;year=1994;volume=26;issue=4;spage=288;epage=291;aulast=Raka;type=0