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Year : 1983  |  Volume : 15  |  Issue : 2  |  Page : 95--100

An in vitro colorimetric method for assay of heparin sodium

DE Mitali1, SC Gupta2, PR Pabrai3 
1 
2 
3 

Correspondence Address:
D E Mitali


A simple and sensitive colorimetric method of assay of heparin sodium based on the comparative determination of anticoagulant activity of the test preparation with that of the standard has been deveIoped. The method involves the assessment of the quantity of heparin required to prevent 50 percent clotting (ED50) of a fixed amount of citrated goat plasma in presence of calcium ions. The method is more precise and rapid than and comparable in accuracy and reproducibility with the USP method. It has the further advantage that the subjectivity inherent in the existing bioassay methods is eliminated.


How to cite this article:
Mitali D E, Gupta S C, Pabrai P R. An in vitro colorimetric method for assay of heparin sodium.Indian J Pharmacol 1983;15:95-100


How to cite this URL:
Mitali D E, Gupta S C, Pabrai P R. An in vitro colorimetric method for assay of heparin sodium. Indian J Pharmacol [serial online] 1983 [cited 2021 Sep 24 ];15:95-100
Available from: https://www.ijp-online.com/article.asp?issn=0253-7613;year=1983;volume=15;issue=2;spage=95;epage=100;aulast=Mitali;type=0