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| RESEARCH ARTICLE |
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| Year : 2018 | Volume
: 50
| Issue : 1 | Page : 12-21 |
Characterization of Rubia cordifolia L. root extract and its evaluation of cardioprotective effect in Wistar rat model
BS Chandrashekar1, S Prabhakara2, T Mohan2, D Shabeer3, Basavaraj Bhandare3, M Nalini4, PS Sharmila4, DL Meghana5, Basanth Kumar Reddy2, HM Hanumantha Rao2, H Sahajananda6, K Anbazhagan7
1 Chemistry of Forest Products (CFP) Division, Institute of Wood Science and Technology, Bangalore, Karnataka, India
2 Department of Genetics, Central Research Laboratory, Bangalore, Karnataka, India
3 Department of Pharmacology, Central Research Laboratory, RajaRajeswari Medical College and Hospital, Bangalore, Karnataka, India
4 Department of Pathology, Central Research Laboratory, RajaRajeswari Medical College and Hospital, Bangalore, Karnataka, India
5 Department of Biological, Sciences Bangalore University, Bangalore, Karnataka, India
6 Department of Anaesthesia and Central Research Laboratory, Central Research Laboratory, RajaRajeswari Medical College and Hospital, Bangalore, Karnataka, India
7 Department of Molecular Medicine, Central Research Laboratory, RajaRajeswari Medical College and Hospital, Bangalore, Karnataka, India
Correspondence Address:
K Anbazhagan
Department of Genetics, Central Research Laboratory, RajaRajeswari Medical College and Hospital, Mysore Road, Bangalore - 560 074, Karnataka
India
S Prabhakara
Department of Genetics, Central Research Laboratory, RajaRajeswari Medical College and Hospital, Mysore Road, Bangalore - 560 074, Karnataka
India
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/ijp.IJP_418_17
OBJECTIVES: Rubia cordifolia L. (RC) is a well-known and highly valuable medicinal plant in the Ayurvedic system. The present study involves evaluating antioxidant and cardioprotective property of RC root extract.
MATERIALS AND METHODS: The characterization of RC root extract was carried out using standard phytochemical and biochemical analysis. The functional groups were analyzed by Fourier transform infrared (FTIR) spectroscopy and phytotherapeutic compounds were identified using high-resolution mass spectrometry (HR-MS). Cardioprotective activity of RC root extract was investigated against cyclophosphamide (CP; 100 mg/kg, i.p)-induced cardiotoxicity in male albino Wistar rats. RC (100, 200, and 400 mg/kg, p.o) or silymarin (100 mg/kg, p.o) was administered immediately after CP on the 1st day and the next consecutive 10 days. Biochemical and histopathological analysis was performed to observe the cardioprotective effects of RC root extract.
RESULTS: Phytochemical analysis revealed the presence of secondary metabolites that include alkaloids, flavonoids, saponins, and anthraquinones in RC root extract. FTIR analysis revealed the presence of several functional groups. Based on HR-MS analysis, eight major phytotherapeutic compounds were identified in methanol root extract of RC. Biochemical analysis in CP-induced rat model administered with RC extract revealed significantly enhanced levels of antioxidant markers such as superoxide dismutase, catalase, and glutathione S-transferase. Histopathological study showed that the rat model treated with the root extract had reduced the cardiac injury.
CONCLUSION: Our results have shown that the RC extract contains various antioxidant compounds with cardioprotective effect. Treatment with RC root extract could significantly protect CP-induced rats from cardiac tissue injury by restoring the antioxidant markers.
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