| RESEARCH ARTICLE
|Year : 2017 | Volume
| Issue : 3 | Page : 229-235
In vitro antioxidant and in vivo antidepressant activity of green synthesized azomethine derivatives of cinnamaldehyde
Sridevi Chigurupati1, Sohrab Akhtar Shaikh2, Jahidul Islam Mohammad3, Kesavanarayanan Krishnan Selvarajan4, Appala Raju Nemala5, Chu How Khaw1, Chun Foo Teoh1, Ting Hei Kee1
1 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, AIMST University, Semeling, 08100 Bedong, Kedah, Malaysia
2 Department of Pharmacology, Faculty of Pharmacy, AIMST University, Semeling, 08100 Bedong, Kedah, Malaysia
3 Department of Pharmacology, Faculty of Medicine, CUCMS, 63000 Cyberjaya, Malaysia
4 Department of Pharmaceutical Pharmacology and Chemistry, Faculty of Pharmacy, Universiti Teknologi MARA, Selangor, Malaysia
5 Department of Pharmaceutical Chemistry, Sultan Ul Uloom College of Pharmacy, Hyderabad, Telangana, India
Objectives: In this study, three (CS-1 to CS-3) azomethine derivatives of cinnamaldehyde were green synthesized, characterized, and their antioxidant and antidepressant activities were explored.
Materials and Methods: The antioxidant effect of these compounds was initially performed in vitro using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay methods before subjecting them to in vivo experiments. Compounds showing potent antioxidant activity (CS-1 and CS-2) were investigated further for their antidepressant activity using the forced swim test (FST) and tail suspension test (TST). Ascorbic acid (AA) and fluoxetine (20 mg/kg, p.o) were used as reference drugs for comparison in the antioxidant and antidepressant experiments, respectively.
Results: It was observed that CS-2 and CS-3 exhibited highest DPPH (half maximal inhibitory concentration [IC50]: 16.22 and 25.18 μg/mL) and ABTS (IC50: 17.2 and 28.86 μg/mL) radical scavenging activity, respectively, compared to AA (IC50: 15.73 and 16.79 μg/mL) and therefore, both CS-2 and CS-3 were tested for their antidepressant effect using FST and TST as experimental models. Pretreatment of CS-2 and CS-3 (20 mg/kg) for 10 days considerably decreased the immobility time in both the FST and TST models.
Conclusion: The antioxidant and antidepressant effect of CS-2 and CS-3 may be attributed to the presence of azomethine linkage in the molecule.
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, AIMST University, Semeling, 08100 Bedong, Kedah
Source of Support: None, Conflict of Interest: None
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