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| ABSTRACTS |
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| Year : 2015 | Volume
: 47
| Issue : 7 | Page : 7-8 |
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OD Gulati Prize
| Date of Web Publication | 11-Dec-2015 |
Correspondence Address:
 Source of Support: None, Conflict of Interest: None  | Check |
How to cite this article:
. OD Gulati Prize. Indian J Pharmacol 2015;47, Suppl S1:7-8 |
OD GULATI-1
Eplerenone Attenuates Cardiac Dysfunction and Oxidative Stress in β-receptor Stimulated Myocardial Infarcted Rats
Umesh Mahajan B 1 , Navya Reddy M 1 , Chandragouda Patil R 1 , Yogeeta Agrawal O 2 , Sameer Goyal N 1
1 Department of Pharmacology, Cardiovascular Pharmacology Division, R.C. Patel Institute of Pharmaceutical Education and Research, Dhule, Maharashtra, India, 2 Department of Pharmaceutics and Quality Assurance, R.C. Patel Institute of Pharmaceutical Education and Research, Dhule, Maharashtra, India
Objectives: The existing cram has been intended to explore, whether eplerenone treatment attenuates the expansion of myocardial infarction in isoproterenol treated rats. Materials and Methods: Myocardial infarction was induced by administration of isoproterenol (100 mg/kg s.c. for 2 consecutive days) in experimental animals. Cardiotoxicity was evidenced by changes observed in hemodynamic, biochemical, and histopathological changes. The eplerenone was administered at the dose of 50, 100 and 150 mg/kg daily for a period of 14 days and studied whether it constructively modulated the hemodynamic, biochemical, and histopathological changes in isoproterenol-induced myocardial injury. Results: Cardiotoxicity was evidenced by marked ST elevation, decrease in systolic, diastolic, mean arterial pressures, maximal positive rate of developed left ventricular pressure (+LVdP/dtmax, a indicator of myocardial contraction), maximal negative rate of developed left ventricular pressure (−LVdP/dtmax, a meter of myocardial relaxation) and an increase in left ventricular end-diastolic pressure (LVEDP, a marker of pre-load). In addition, a significant reduction in activities of myocardial creatine kinase-MB isoenzyme, lactate dehydrogenase, superoxide dismutase, catalase, and reduced glutathione level along with increase in malondialdehyde content were observed. Eplerenone attenuated all the parameters dose dependently, and at doses of 100 mg/kg and 150 mg/kg produced more pronounced protective effects than 50 mg/kg body weight. Conclusion: Present study thus provides evidence for protective effects of eplerenone on myocardium in experimentally induced myocardial infarction.
OD GULATI-2
Dietary Antioxidants of Macrotyloma Uniflorum Protect the Rat Heart Against Isoproterenol Induced Myocardial Infarction: An Electrocardiographic, Hemodynamic, Biochemical and Histoarchitectural Study
Vandana Panda 1 , Ankit Laddha 1 , Mukesh Nandave 2
1 Department of Pharmacology and Toxicology, Prin. K. M. Kundnani College of Pharmacy, Colaba, Mumbai, Maharashtra, India, 2 Department of Pharmacology, SPP School of Pharmacy and Technology Management, SVKM's NMIMS, Vile Parle (W), Mumbai, Maharashtra, India
Objectives: Macrotyloma uniflorum (horse gram) is an underutilized and unexplored legume distributed throughout Asia and rich in antioxidant phenolic acids. The present study investigates the cardioprotective effect and antioxidant activity of a polyphenol rich extract of Macrotyloma uniflorum seeds (MUSE) and its constituent phenolic acids, viz., ferulic and p-coumaric acid in isoproterenol (ISO) induced myocardial infarction in rats. Methods and Results: Isolation and quantification of the above phenolic acids from MUSE was carried out by HPTLC and HPLC respectively. Pretreatment of MUSE (250 & 500 mg/kg, po) and the phenolic acids daily for 30 days to rats treated with ISO (85 mg/kg, sc) on the last 2 days, resulted in a significant cardio protective activity reflected by attenuation of the ISO-elevated levels of serum marker enzymes (AST, LDH and CK-MB), total cholesterol, triglycerides, uric acid, C-reactive protein and malondialdehyde, and a restoration of the levels of the ISO-depleted marker enzymes, reduced glutathione and antioxidant enzymes - superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase in the heart. Restoration of the ISO altered hemodynamic parameters (left ventricular end diastolic pressure, heart rate, systolic, diastolic and mean arterial pressure) and ECG was also brought about by treatment with MUSE and the phenolic acids. Histopathological studies of the heart corroborated the above results. Conclusion: It may be concluded that MUSE oral treatment to ISO challenged rats augments endogenous antioxidants of rat heart, enhances scavenging of free radicals and inhibits lipid peroxidation of the myocardial membrane, thereby salvaging the myocardium from the deleterious effects of ISO.
OD GULATI-3
Evaluation of Cardioprotective Effect of Aquous Extract of Allium cepa Linn. Bulb (Onion) on Isoprenaline-induced Myocardial Injury in Wistar Albino Rats
Kharadi GB, Patel KJ, Purohit B, Baxi S, Tripathi CB
Department of Pharmacology, Government Medical College, Bhavnagar, Gujarat, India
Objectives: To investigate the cardioprotective potential of the aqueous extract of Allium cepa Linn. bulb in isoprenaline-induced myocardial injury in Wistar albino rats. Materials and Methods: In vitro total phenolic, total flavonoid content and 2,2'-diphenyl-1-picrylhydrazyl hydrate radical scavenging activity was measured. Isoprenaline-induced myocardial injury model was used to evaluate in vivo effect of aqueous extract of A. cepa in Wistar albino rats. Seventy two rats were randomly divided in 6 groups. Rats were treated with A. cepa 400 mg/kg and 800 mg/kg doses for 30 days and myocardial injury was produced by subcutaneous injection of isoprenaline (ISO) 85 mg/kg on day 28 and 29. Carvedilol 1 mg/kg for 30 days served as active control. Electrocardiogram parameters, cardiac injury markers, oxidative stress markers and histopathological changes were evaluated in each group and compared using appropriate statistical tests. Results: In vitro evaluation of aqueous extract of A. cepa showed significant antioxidant property. Isoprenaline-produced significant myocardial injury as compared to normal control group (P < 0.05). Administration of A. cepa in the dose 400 mg/kg significantly recovered the altered parameters (Trop-I, CK-MB, SGPT, HR, R-R interval, and Oxidative stress markers) compared to disease control group (P < 0.05) while A. cepa in the dose 800 mg/kg recovered the altered parameters (HR, HW/BW ratio and SOD level) compared to disease control group. Histopathological parameters did not recovered in the doses of 400 and 800 mg/kg (P > 0.05). Conclusion: The aqueous extract of A. cepa 400 mg/kg was found to be cardioprotective against myocardial injury while A. cepa 800 mg/kg not showed significant cardioprotective activity. So, we presume that A. cepa might be effective within certain dose range only.
OD GULATI-4
MCR-1329, Novel Dual 6,7-dimethoxyquinazoline Antagonist (α1 and at 1 receptors) Prevents Hypertension in Rats
Sagar Patel, Hardik Gandhi, Prashant Naik, Kirti Patel, Yadav MR
Department of Pharmacy, Faculty of Technology and Engineering, The M.S. University of, Vadodara, Gujarat, India
Objectives: Uncontrolled hypertension is one of the major risks for cardiovasuclar morbidity and mortality. Objective of this study include to evaluation of novel dual receptor antagonist that can exhibit superior pharmacological activity with predictable pharmacokinetic profile by inhibiting both highly involved receptor systems with single entity. Materials and Methods: Potent compound was selected for acute toxicity according to OECD guidelines. MCR-1329 (10 mg/kg) was evaluated in unilateral nephrecotomized +DOCA salt-induced hypertensive rats. Various heamodynamics parameters were studied. Different serum and urine parameters were studied using different biochemical test and commercially available kits. Results: Previously we have established the in vitro potency of MCR-12329 on rat aortic strips and its ability to inhibit pressor response elicited by standard agonist in invasive blood pressure monitoring. In continuation of this study toxicological evaluations were performed by administration of the test compound, acute toxicity study revealed that the compound showed no signs of toxicity at a single dose administration of 2000 mg/kg. Results of the study demonstrated that administration of MCR-1329 could prevent development of hypertension mediated through salt intake and aldosterone turnover along with endothelia dysfunction. Electrolyte imbalance and urinary markers of hypertension were also controlled by MCR-1329 as indicated by urinary indices. Conclusion: The results of this study indicate that safe and efficacious multiply-targeted ligands can be designed to show an evenhanded modulation of the desired targets to achieve therapeutic goals. Further, the compounds presented in the present study may serve as potential examples or lead candidates who could be further explored for multiply targeting complex disorders.
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