| RESEARCH ARTICLE
|Year : 2013 | Volume
| Issue : 5 | Page : 479-482
Ethanolic extract of Piper betle Linn. leaves reduces nociception via modulation of arachidonic acid pathway
Soumita De, Niteeka Maroo, Piu Saha, Samik Hazra, Mitali Chatterjee
Department of Pharmacology, Institute of Postgraduate Medical Education & Research, Kolkata, West Bengal, India
Objectives: The objective of this study was to evaluate the peripheral analgesic effect of Piper betle leaf extract (PBE) along with establishing its putative mechanism of action.
Materials and Methods: Male Swiss albino mice after pre-treatment (1 h) with different doses of PBE were injected 0.8% (v/v) acetic acid i.p.; the onset and number of writhes were noted up to 15 min. To evaluate the mechanism of action, the murine peritoneal exudate was incubated with PBE for 1 h, followed by exposure to arachidonic acid (AA) and generation of reactive oxygen species (ROS) was measured by flow cytometry using 2',7'-dichlorodihydrofluorescein diacetate.
Results: PBE in a dose dependent manner significantly reduced acetic acid induced writhing response in mice (P < 0.001). In peritoneal exudates, PBE significantly inhibited AA induced generation of ROS, P < 0.01.
Conclusions: The present study indicates that PBE has promising analgesic activity, worthy of future pharmacological consideration.
Department of Pharmacology, Institute of Postgraduate Medical Education & Research, Kolkata, West Bengal
Source of Support: The work received financial assistance from the Department of Biotechnology and Board of Research in Nuclear Sciences, Govt. of India., Conflict of Interest: None
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