| RESEARCH ARTICLE
|Year : 2013 | Volume
| Issue : 3 | Page : 227-231
Differences in morphine-induced antinociception in male and female offspring born of morphine exposed mothers
Masoomeh Biglarnia1, Manizheh Karami2, Zahra Khodabakhshi Hafshejani1
1 Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran
2 Department of Biology, Faculty of Basic Sciences, Shahed University; Neurophysiology Research Center of Shahed University, Tehran, Iran
Objective: Antinociceptive effect of morphine in offspring born of mothers that received saline or morphine during the gestation period was investigated.
Materials and Methods: Wistar rats (200-250 g) received saline, morphine 0.5 mg/kg or 5 mg/kg during gestation days 14-16. All pups after weaning were isolated treatment/sex dependently and were allowed to fully mature. The antinociceptive effect of morphine was assessed in formalin test. Morphine (0.5-7.5 mg/kg) or saline (1 ml/kg) was injected intraperitoneally 10 min before formalin (50 μl of 2.5% solution in right hind-paw).
Results: Male offspring born of saline-treated mothers were less morphine-sensitive than females. On the contrary, male offspring exposed prenatally to morphine (5 mg/kg) were more sensitive to morphine-induced antinociceptive response in formalin test. However, no difference in antinociceptive effect was observed amongst offspring of either sex born of mothers treated with morphine 0.5 mg/kg, identifying a lower dose effect of the opioid.
Conclusion: The exposure to morphine during the developmental period may result in altered development of tolerance to morphine and thus involved in drug abuse.
Department of Biology, Faculty of Basic Sciences, Shahed University; Neurophysiology Research Center of Shahed University, Tehran
Source of Support: None, Conflict of Interest: None
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