| RESEARCH ARTICLE
|Year : 2012 | Volume
| Issue : 4 | Page : 469-474
Platycodon grandiflorus alleviates DNCB-induced atopy-like dermatitis in NC/Nga mice
Sang-Joon Park1, Hyang-Ae Lee2, Jong Woo Kim3, Byoung-Seok Lee4, Eun-Joo Kim4
1 College of Veterinary Medicine, Kyungpook National University, Buk-gu, Daegu, Korea
2 Korea Institute of Toxicology, Korea Research Institute of Chemical Technology, Yuseong-gu, Daejeon; Department of Physiology, Seoul National University, Jongro-gu, Seoul, Korea
3 B and C Biopharm Co. Ltd., Yeongtong-gu, Suwon, Korea
4 Korea Institute of Toxicology, Korea Research Institute of Chemical Technology, Yuseong-gu, Daejeon, Korea
Objective: To investigate the therapeutic effect of crude extract from Platycodon grandiflorum (PG) roots on atopic dermatitis (AD)-like skin lesions in NC/Nga mice.
Materials and Methods: To develop atopic dermatitis-like lesions, 200 μl of 0.3% 1-chloro-2, 4-dinitro benzene (DNCB) in acetone/olive oil (3:1) was applied 3 times a week for 2 weeks on the shaved skin of their backs. PG extract was dissolved in saline and orally administrated at concentrations of 300 and 500 mg/kg every day for 2 weeks. The therapeutic effect of PG on AD-like skin lesions was assessed by measuring skin severity scores and epithermal thickness, serum total immunoglobulin (Ig) E, histopathological findings for inflammatory cells including mast cells, macrophage and T cells, and mRNA expression of various cytokines related to the inflammatory and allergic response. The significance of inter-group differences was analyzed using the ANOVA test. Data were considered to be significant when P < 0.05 or P < 0.01.
Results: Oral treatment of PG suppressed AD-like skin lesions according to the assessment of skin severity and epithermal thickness in the DNCB-treated NC/Nga mice. This alleviation was further correlated with a reduction of elevated serum total IgE or cytokine mRNA in the PG-treated group compared with vehicle-treated positive group. In addition, infiltrated inflammatory cells decreased on the skin lesions compared with vehicle-treated group.
Conclusion: These results suggest that PG may have a potential therapeutic effect for AD via the inhibition of both inflammatory and allergic reaction.
Korea Institute of Toxicology, Korea Research Institute of Chemical Technology, Yuseong-gu, Daejeon
Source of Support: Grant from the Next-Generation BioGreen 21 Program (No. PJ 008017), Rural Development Administration, Republic of Korea, Conflict of Interest: None
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