| RESEARCH ARTICLE
|Year : 2012 | Volume
| Issue : 4 | Page : 448-453
The in vitro anti-viral potential of Setarud (IMOD™), a commercial herbal medicine with protective activity against acquired immune deficiency syndrome in clinical trials
Rezvan Zabihollahi1, Rahele Namazi2, Mohammad Reze Aghasadeghi1, Azar Farhang Esfahani3, Seyed Mehdi Sadat1, Mohammad Hossein Modarressi4
1 Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran
2 Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan, Iran
3 Member of Young Researchers Club, Islamic Azad University, Falavarjan Branch, Falavarjan, Iran
4 Faculty of Medicine, Tehran University of Medical Sciences TUMS, Tehran, Iran
Objectives: Setarud (IMOD™) is a herbal medicine with beneficial effect for patients suffering Human immunodeficiency virus (HIV) infection and has been approved for IV (intra venues) injection. The beneficial effect of IMOD administration for acquired immune deficiency syndrome (AIDS) patient has been proved in previous clinical trials. Here the in vitro inhibitory effect of IMOD against HIV-1, Herpes simplex virus (HSV) and murine leukemia viruses (MLV) was evaluated.
Materials and Methods: HIV single cycle replication and HSV plaque reduction assays were used to evaluate the anti-viral effect. The level of HIV replication was monitored by p24 capture Enzyme-linked immunosorbent assay (ELISA). The single round infection [with green fluorescent protein (GFP) reporter MLV and HIV], virucidal and time-of-additions (HSV) assays were utilized to determine the mode of anti-viral activity. The toxicity of IMOD for cells was monitored by XTT (sodium 3_-[1 (phenylaminocarbonyl)- 3,4-tetrazolium]-bis (4-methoxy-6-nitro)benzene sulfonic acid) cell proliferation assay kit.
Results: IMOD inhibited 50% of HIV-1 and HSV replication (IC 50 ) at 6.5 × 10 -4 and 4.3 × 10 -3 V/V concentrations, respectively. The IC 50 value against HIV-1 and MLV infection were 6 × 10 -4 V/V and 4.9 × 10 -4 V/V. Virucidal assay showed that IMOD reduces the potency of HIV and HSV particles to 41 and 54% of control, respectively. Time-of-addition study revealed that IMOD inhibits the replication of HSV at a stage after penetration of virions to the target cells.
Conclusions: Data from this study indicate that IMOD has significant anti-viral activity against HIV, HSV and MLV. Setarud could be subjected to further investigation after isolation of the constituents and determination of the toxic components.
Mohammad Hossein Modarressi
Faculty of Medicine, Tehran University of Medical Sciences TUMS, Tehran
Source of Support: Pasteur institute of Iran, Conflict of Interest: None
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