| RESEARCH ARTICLE |
|
| Year : 2011 | Volume
: 43
| Issue : 5 | Page : 546-550 |
Evaluation of the concomitant use of methotrexate and curcumin on Freund's complete adjuvant-induced arthritis and hematological indices in rats
David Banji, Jyothi Pinnapureddy, Otilia J.F Banji, A Ranjith Kumar, K Narsi Reddy
Department of Pharmacology, Nalanda College of Pharmacy, Nalgonda, Andhra Pradesh, India
Correspondence Address:
David Banji
Department of Pharmacology, Nalanda College of Pharmacy, Nalgonda, Andhra Pradesh
India
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/0253-7613.84970
Objective : To evaluate the concomitant administration of methotrexate and curcumin for antiarthiritic activity in rats. Materials and Methods : Arthritis was induced in rats following a single subplantar injection of Freund's complete adjuvant (0.1 ml). Rats were divided into six groups of six animals each. Group I and II were control injected with saline and Freund's complete adjuvant (0.1 ml), respectively. Group III arthritic rats were treated with curcumin (100 mg/kg, i.p.) on alternate days. Group IV received methotrexate (MTX) (2 mg/kg, i.p.) once in a week. Group-V and VI were treated with MTX (1 mg/kg, i.p.) once in a week and after 30 min received curcumin (30 mg/kg and 100 mg/kg, thrice a week, i.p.) from 10 th to 45 th days, respectively. Body weight and the paw volume was measured on 9 th , 16 th , 23 rd , 30 th , 37 th , and 45 th days. Determination of complete blood cell counts, hemoglobin concentration, hematocrit, mean corpuscular volume, and mean corpuscular hemoglobin concentration was determined on the 46 th day. Results : An improvement in body weight and a significant (P < 0.05) reduction in arthritis was observed with the combination treatment as compared to the positive control. A significant improvement in the hematological profile was also observed in rats treated with curcumin and methotrexate. Conclusion : The study showed a significant anti-arthritic action and protection from hematological toxicity with the combination treatment of methotrexate and curcumin.
[FULL TEXT] [PDF]*
|
