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| Year : 2011 | Volume
: 43
| Issue : 3 | Page : 336-339 |
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Antiulcer and antioxidant activity of ethanolic extract of Passiflora foetida L.
R Sathish, Alok Sahu, K Natarajan
Department of Pharmacology, Ultra College of Pharmacy, Madurai, Tamil Nadu, India
| Date of Submission | 04-Oct-2010 |
| Date of Decision | 21-Jan-2011 |
| Date of Acceptance | 08-Feb-2011 |
| Date of Web Publication | 24-May-2011 |
Correspondence Address:
R Sathish
Department of Pharmacology, Ultra College of Pharmacy, Madurai, Tamil Nadu
India
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/0253-7613.81501
Passiflora foetida L. (Passifloraceae), a widely growing perennial climber, has been used in traditional medicine for treating many ailments. The objective of the present study was to evaluate the effects of ethanolic extract of P. foetida (EEPF) whole plant on gastric ulcer. The antiulcer effects of EEPF at 100 and 200 mg/kg doses were evaluated on ethanol and aspirin-induced gastric ulcer models. The antioxidant parameters and histological changes in gastric tissue of ulcer rats were also determined in both the models. P. foetida treatment significantly (P < 0.01) reduced the ulcer index and significantly (P < 0.01) increased the gastric pH of both ethanol and aspirin-induced ulcer rats. P. foetida showed significant (P < 0.01) reduction in lipid peroxidation and increase in reduced glutathione levels. The observations confirm that EEPF whole plant has antiulcer and antioxidant activities.
Keywords: Antioxidant, antiulcer activity, Passiflora foetida
How to cite this article:
Sathish R, Sahu A, Natarajan K. Antiulcer and antioxidant activity of ethanolic extract of Passiflora foetida L. Indian J Pharmacol 2011;43:336-9 |
How to cite this URL:
Sathish R, Sahu A, Natarajan K. Antiulcer and antioxidant activity of ethanolic extract of Passiflora foetida L. Indian J Pharmacol [serial online] 2011 [cited 2023 Mar 30];43:336-9. Available from: https://www.ijp-online.com/text.asp?2011/43/3/336/81501 |
| » Introduction | |  |
Gastric ulcer is a break in the tissue lining of the stomach. Most ulcers can be cured without complications; however, in some cases peptic ulcers can develop, such as in penetration, perforation, bleeding (hemorrhage), and obstruction. [1],[2] Ethanol and aspirin-induced gastric ulcer models have been widely used for the evaluation of gastroprotective activity. Acute treatment with ethanol increases oxidative stress, DNA damage, xanthine oxidase activity and malondialdehyde levels, and decreases the total glutathione content in gastric mucosal cells. [3] Aspirin-induced ulcer is mediated through tissue damaging free radicals which are produced from the conversion of hydroperoxyl to hydroxy fatty acids, which leads to cell destruction. [4] It has been found that oxygen-derived free radicals are implicated in the mechanism of acute and chronic ulceration in the gastric mucosa and scavenging these free radicals can play an appreciable role in healing the ulcer. [5] Before the introduction of potent antiulcerogenic agents, i.e., H 2 -receptor antagonist, proton pump inhibitors, etc., plant remedies were widely employed for the treatment of various symptoms of peptic ulcer. There has been renewed interest in identifying new antiulcer drugs from natural sources. Passiflora foetida L. (Passifloraceae), commonly known as passion fruit, is an exotic fast growing perennial climber occurring in USA and extended to India. Traditionally, the plant has been used for its properties like antiproliferative, sedative, anti-anxiety, antibacterial, leishmanicidal, antispasmodic, emetic, dressing for wounds and antiulcer. [6],[7],[8] In this preliminary study, an attempt has been made to evaluate the effects of P. foetida whole plant on experimentally induced gastric ulcers.
| » Materials and Methods | | |
Extraction and Qualitative Chemical Evaluation
The plants were collected from Azhagar kovil, and were identified and authenticated by Dr. D. Stephen, Taxonomist, American College, Madurai. About 300 g of the plant powder was extracted with 1.5 l of ethanol at room temperature for 7 days and the mixture was stirred every 24 h. [8] The ethanolic extract of P. foetida (EEPF) was evaporated (at 40°C) and the sticky blackish green substance (3.73%) was subjected to qualitative chemical tests to evaluate the various phytoconstituents. [9]
Animals
Wister rats of either sex (150-200 g) were properly housed in separate cages at controlled room temperature (24 ± 2°C; relative humidity 60-70%) in a 12-h light dark cycle. They were fed with standard pellet diet and water ad libitum. The IAEC of Ultra College of Pharmacy permitted this pharmacological study (approval no. UCP/IAEC/ 2008/030).
Antiulcer Activity
Ulcer induced by absolute ethanol
The rats were divided into four groups of six each. Group I (toxicant control) received absolute ethanol (1 ml/animal); Group II was treated with ranitidine (100 mg/kg); Groups III and IV were treated with EEPF 100 and 200 mg/kg, respectively. [10] The rats were fasted for 24 h and they received 1 ml of absolute ethanol orally. Standard and test drugs were administered orally 30 min before the ethanol dose. The animals were sacrificed after 1 h of ulcerogen administration, and their stomachs were excised and the gastric contents were aspirated. The contents were subjected to centrifugation at 1000 rpm for 10 min and then analyzed for pH (digital pH meter). The stomachs were washed with normal saline and kept in 10% formalin for the determination of ulcer index and histological studies. [11],[12]
Ulcer index is determined as follows:
Ulcer index = 10/x, where "x" is total mucosal area/total ulcerated area.
Ulcer induced by aspirin
The rats were divided into four groups of six each like the absolute ethanol-induced ulcer models, but the control and drug-treated animals received aspirin (100 mg/kg p.o.) instead of ethanol. The rats were fasted for 24 h with free access to water, the standard and test drugs were administered orally 30 min before the aspirin dose. [13] The animals were sacrificed after 5 h of ulcerogen administration and their stomachs were excised, then the procedure followed was similar to that for absolute ethanol-induced ulcer model.
Histopathological Evaluation
After the standard processing, the ulcerated gastric tissues were examined under the microscope for histopathological changes such as inflammation, infiltration, and erosion. [5]
Antioxidant Activity
The lipid peroxides (LPOs) of stomach mucosa were determined indirectly by thiobarbituric acid reactive substances (TBARS) formation. Reduced glutathione concentration was read off a standard curve and expressed as μg GSH/g of wet tissue. Alkaline phosphatase activity was assayed using the method of Kind and King. [14],[15],[16]
Statistical Analysis
The treated groups were compared with the toxicant group; results were expressed as a mean ± SD of six animals in each group. The results were analyzed statistically using one-way analysis of variance (ANOVA) followed by Dunnett's test. P < 0.05 when compared with toxicant group was considered as significant.
| » Results | | |
The phytochemical screening of the EEPF showed the presence of alkaloids, phenols, carbohydrates, proteins, phytosterols, phenolic compounds, tannins, and flavonoids. In ethanol-induced ulcer model, EEPF significantly (P < 0.01) reduced the ulcer index at 100 and 200 mg/kg doses to 64.30 and 71.66%, respectively. In aspirin-induced ulcer model, the EEPF treatment at 100 and 200 mg/kg significantly (P < 0.01) inhibited the ulcer index to 67.83 and 79.45%, respectively, when compared with toxicant control rats. The EEPF also significantly (P < 0.01) increased gastric pH of ulcer rats [Table 1]. In both the ulcer models, EEPF (100 and 200 mg/kg) significantly (P < 0.01) reduced the lipid peroxidation and alkaline phosphatase levels when compared to toxicant group. The results also show that EEPF significantly (P < 0.01) increased the reduced glutathione levels [Table 2]. Histopathological evaluation of alcohol and aspirin-induced ulcer models showed perforated ulcer, deep ulceration of granular epithelium and loss of the histological structure, almost reducing the submucosa. EEPF at 100 mg/kg showed partial healing of ulcer with few inflammatory cells and at 200 mg/kg showed the healed ulcer, normal mucosa, and no inflammatory cells [Figure 1] and [Figure 2].  | Table 1: Effect of Passiflora foetida L. on pH and ulcer index of absolute ethanol and aspirin-induced ulcer models
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 | Table 2: Antioxidant effect of Passiflora foetida L. on absolute ethanol and aspirin-induced ulcer models
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 | Figure 1: Histopathology of stomach of absolute ethanol-induced ulcer models
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| » Discussion | | |
The genesis of ethanol-induced gastric lesions is multifactorial with the depletion of gastric wall mucous content as one of the involved factors. It is also associated with significant production of free radicals, leading to an increased oxidative stress and damage to the cell and cell membrane. [17] Aspirin causes a dose-dependent reduction in mucosal prostaglandin E 2 and prostaglandin I 2 biosynthesis accompanied by an increase in the mean of gastric ulceration. It is therefore reasonable to assume that the observed gastric mucosal lesion induced by aspirin is due to deficiency of mucosal prostaglandin. [18] The present study reveals that both the doses of EEPF significantly (P < 0.01) reduced the ulcer index and increased the gastric pH of ethanol and aspirin-induced ulcer models. Lipid peroxidation is a free radical mediated process, which has been implicated in a variety of disease states. It involves the formation and propagation of free radicals, the uptake of oxygen and rearrangement of double bond in unsaturated lipids, which eventually result in destruction of membrane lipids. The release of alkaline phosphatase enzyme has been suggested to play a role in tissue necrosis associated with various models of gastrointestinal ulceration such as absolute alcohol and aspirin-induced ulcer. Increased activity of this enzyme may be found in damaged tissues. [16] EEPF at both the dose levels significantly (P < 0.01) reduced the LPOs and alkaline phosphatase levels of ulcer animals. Antioxidant enzymes such as glutathione are present in oxygen handling cells, which are the first line of cellular defense against oxidative injury. They decrease the gastric mucosal damaging effect of absolute alcohol and aspirin. There was a significant (P < 0.01) increase of glutathione activity in EEPF pretreated rats. Hence, the antioxidant activity of EEPF may be one of the important defensive factors involved in its antiulcer effect. The present study reveals that P. foetida exhibits both antiulcer and antioxidant properties.
| » Conclusion | | |
To conclude, the ethanolic extract of P. foetida Linn. has been found to have antiulcerogenic effect, which could be related to its antioxidant potential. More work is required for a clear understanding of the mechanism of action with chemically identified active principle.
| » References | | |
| 1. | Kochman ML, Elta GH. Gastric ulcers - when is enough, enough. Gastroenterology 1993;105:1583. 
|
| 2. | Chan FK, Leung WK. Peptic ulcer disease. Lancet 2002;360:933-41.
[PUBMED] [FULLTEXT] |
| 3. | Cola Miranda M, Barbastefano V, Hiruma-Lima CA, Calvo TR, Vilegas W, Brito AR. Antiulcerogenic activity of indigofera truxillensis kunth. Biota Neotrop 2006;6:3.
|
| 4. | Sannomiya M, Fonseca VB, Dasilva MA, Rocha LR, Dossantos LC, Hiruma-Lima CA, et al. Flavonoids and antiulcerogenic activity from Brysonima crassa leaves extracts. J Ethnopharmacol 2005;97:1-6.
|
| 5. | Umamaheshwari M, Asokkumar K, Rathidevi R, Sivashanmugam AT, Subhadra DV, Ravi TK. Antiulcer and in vitro antioxidant activities of Jasminum grandiflorum L. J Ethnopharmacol 2007;110:464-70.
|
| 6. | Blumenthal M, editor. Klein, trans. German Commission Therapeutic Monographs on Medicinal Herbs for Human Use. Austin, Texas: American Botanical Council, America; 1997.
|
| 7. | Nadkarni KM. Indian Materia Medica vol 1, 1996; p.924.
|
| 8. | Mohanasundari C, Natarajan D, Srinivasan K, Umamaheshwari S, Ramachandran A. Antibacterial properties of Passiflora foetida a common exotic medicinal plant. Afr J Biotechnol 2007;6:2650-3.
|
| 9. | Khandelwal KR. Practical Pharmacognosy. 11 th ed. Pune: Nirali Prakashan; 2004. p. 149-56.
|
| 10. | Anandan R, Jayakar B, Jeganathan S, Manavalan R, Senthilkumar R. Effect of ethanol extract of fruits of Passiflora foetida Linn. on CCl4 induced hepatic injury in rats. J Pharm Res 2009;2:413-5.
|
| 11. | Morimoto M, Shimohara K, Oshima S, Sukamoto T. Effects of the new anti ulcer agent KB-5492 on experimental gastric mucosal lesions and gastric mucosal defensive factors, as compared to those of teprenone and cimitidine. Jpn J Pharmacol 1991;57:495-505.
|
| 12. | Sing R, Madan J, Rao HS. Anti ulcer activity of black pepper against absolute ethanol induced gastric mucosal damage in mice. Pharmacog Mag 2008;4:232-5.
|
| 13. | Muniappan M, Sundarraj T. Anti-inflammatory and antiulcer activites of Bambusa arundinacea. J Ethnopharmacol 2003;88:161-7.
|
| 14. | Khalil H, Ismail H, Taye A, Kamel M. Gastroprotective effect of Lippa nodiflora L. extracts in ethanol induced gastric lesions. Pharmacog Mag 2007;3:973-1296.
|
| 15. | Sedlak J, Lindsay RH. Estimation of total protein- bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Anal Biochem 1968;25:1192-205.
|
| 16. | Kind PR, King EJ. Determination of serum alkaline phosphatase. Clin Path 1954;7:322.
|
| 17. | Narayan S, Devi RS, Jainu M, Sabitha KE, Shyamala DC. Protective effect of a polyherbal drug ambrex in ethanol induced gastric mucosal lesions in experimental rats. Indian J Pharmacol 2003;36:34-7.
|
| 18. | Ologundudu A, Lawal AO. The antiulcerogenic activity of aqueous extract of Carica papaya fruits on aspirin induced ulcer in rats. Int J Toxicol 2008;5:2.
|
[Figure 1], [Figure 2]
[Table 1], [Table 2]
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