IPSIndian Journal of Pharmacology
Home  IPS  Feedback Subscribe Top cited articles Login 
Users Online : 12501 
Small font sizeDefault font sizeIncrease font size
Navigate Here
Resource Links
 »  Similar in PUBMED
 »  Search Pubmed for
 »  Search in Google Scholar for
 »Related articles
 »  Article in PDF (447 KB)
 »  Citation Manager
 »  Access Statistics
 »  Reader Comments
 »  Email Alert *
 »  Add to My List *
* Registration required (free)

In This Article
 »  Abstract
 »  Introduction
 »  Material and Methods
 »  Results
 »  Discussion
 »  References
 »  Article Figures
 »  Article Tables

 Article Access Statistics
    PDF Downloaded388    
    Comments [Add]    
    Cited by others 6    

Recommend this journal


 Table of Contents    
Year : 2011  |  Volume : 43  |  Issue : 2  |  Page : 200-202

Nephroprotective effect of Bauhinia variegata (linn.) whole stem extract against cisplatin-induced nephropathy in rats

1 University Department of Pharmaceutical Sciences, Utkal University, Bhubaneswar-751 004, Orissa, India
2 K. V. Virani Institute of Pharmacy and Research Centre, Badhada, Savarkundla - 364 515, Gujurat, India
3 Phytochemistry and Pharmacognosy Division, University Department of Pharmaceutical Sciences, Utkal University, Bhubaneswar-751 004, Orissa, India

Date of Submission18-Jun-2010
Date of Decision19-Aug-2010
Date of Acceptance30-Dec-2010
Date of Web Publication6-Mar-2011

Correspondence Address:
Sabuj Sahoo
Phytochemistry and Pharmacognosy Division, University Department of Pharmaceutical Sciences, Utkal University, Bhubaneswar-751 004, Orissa
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0253-7613.77370

Rights and Permissions

 » Abstract 

The nephroprotective activity of the ethanolic extract of Bauhinia variegata (Linn.) whole stem against cisplatin-induced nephropathy was investigated by an in vivo method in rats. Acute nephrotoxicity was induced by i.p. injection of cisplatin (7 mg/kg of body weight (b.w.)). Administration of ethanol extract at dose levels of 400 and 200 mg/kg (b.w.) to cisplatin-intoxicated rats for 14 days attenuated the biochemical and histological signs of nephrotoxicity of cisplatin in a dose-dependent fashion. Ethanol extract at 400 mg/kg decreased the serum level of creatinine (0.65 ± 0.09; P<0.001) and urea (32.86 ± 5.88; P<0.001) associated with a significant increase in body weight (7.16 ± 1.10; P<0.001) and urine volume output (11.95 ± 0.79; P<0.05) as compared to the toxic control group. The ethanol extract of B. variegata at 400 mg/kg (b.w.) exhibited significant and comparable nephroprotective potential to that of the standard polyherbal drug cystone. The statistically (one-way-ANOVA followed by Tukey-Kramer multiple comparison) processed results suggested the protective action of B. variegate whole stem against cisplatin-induced nephropathy.

Keywords: Cisplatin, nephrotoxicity, Kanchana

How to cite this article:
Pani SR, Mishra S, Sahoo S, Panda PK. Nephroprotective effect of Bauhinia variegata (linn.) whole stem extract against cisplatin-induced nephropathy in rats. Indian J Pharmacol 2011;43:200-2

How to cite this URL:
Pani SR, Mishra S, Sahoo S, Panda PK. Nephroprotective effect of Bauhinia variegata (linn.) whole stem extract against cisplatin-induced nephropathy in rats. Indian J Pharmacol [serial online] 2011 [cited 2023 Sep 22];43:200-2. Available from: https://www.ijp-online.com/text.asp?2011/43/2/200/77370

 » Introduction Top

Nephropathy is widely encountered among the people of entire world irrespective of the age, racial, environmental, and geographical variability. The etiology behind this complication is broad ranging from substance-induced to various metabolic and physiological disturbances, paneling nephropathy amongst the 10 leading causes of death across the world. Cisplatin (cis-diaminedichloroplatinum II, CDDP) is extensively used for the treatment of several cancers like testicular and lungs cancer. Unfortunately, the gracious drug cisplatin is conjoined with a brutal side effect since it induces nephrotoxicity. [1]

The plant Bauhinia variegata (BV) Linn., locally known as Kanchana, [2] belonging to the family Caesalpiniaceae, is a widely distributed plant throughout the subtropical and tropical regions of the world. It is used in abundance for medicinal and cattle feed purposes by the tribes of India [3] and also being used in various indigenous systems of medicine like Ayurveda [4] and Unani. Different parts of this plant are used traditionally in various ailments owing to its folkloric claims such as liver tonic, antibacterial, [5] to suppress the edema, dysentery, ulcers, eye disease, skin diseases, piles, and hemorrhoids. [6] The stem of this plant is reported to contain many active phytochemicals including stigmasterol, flavone glycosides, lupeol, kaempferol-3-glucoside, β-setosterol, etc. [7] In this present context, the in vivo nephroprotective activity of the ethanolic extract of B. variegata (BV) whole stem was evaluated in albino male rats (Wistar strain).

 » Material and Methods Top

The whole stem of BV was collected from young matured plant from Bharatpur Forest Range, Bhubaneswar, Orissa, India, during the month of November and December and identified by Dr. B. K. Jaysingh., Gopabandhu Ayurvedic College, Puri, Orissa. The plant specimen (Vide Voucher No.: 081) was deposited in herbarium of University Department of Pharmaceutical Sciences, Bhubaneswar, Orissa, India. After authentication fresh plant materials were collected in bulk, washed under running tap water to remove adherents, shade dried, and pulverized in a mechanical grinder to get coarse powder of BV whole stem.

Healthy albino male rats of Wistar strain weighing between 150 and 200 g were selected for the investigation. The animals were kept under maintained laboratory conditions with adequate supply of drinking water ad libitum and pallet diet. The experimental protocol was approved by the Institutional Animal Ethics Committee and the conditions in the animal house approved by Committee for Supervision on Experiments on Animals (Vide Registration No.: 990/c/06/CPCSEA).

About 200 g of coarse powder of BV whole stem was taken in a soxhlet apparatus and extracted [8] successively with petroleum ether, chloroform, and ethanol. The extraction for each solvent was carried out for 18--20 h. The ethanol extract was collected by evaporating the solvent by slow heat treatment. Total 1.4 kg of pulverized stem was used to produce the required amount of test extract. Calculated amount of dried ethanol extract was suspended in 0.5% w/v of sodium CMC in a normal saline solution to prepare the test doses (200 and 400 mg/kg/ml.). The dose limits were selected on the basis of previously performed oral acute toxicity studies in mice, in accordance with the OECD guidelines. [9]

Total 30 Wistar rats were divided randomly into five groups of six animals each. Group I (normal control) received oral dose of 0.5% sodium CMC (1 ml each) for 14 days. Group II (toxic control) received single dose of cisplatin [10] (7 mg/kg of body weight; i.p.) on day 1. Group III (standard group) received standard polyherbal drug cystone [11] (5 ml/kg; p.o.) (Cystone Syrup, Himalaya Drug Company., Bangalore, India) for 14 days with single dose of cisplatin (7 mg/kg of body weight; i.p.) on day 1, group IV (EEBV 200 ) and group V (EEBV 400 ) received ethanolic extract 200 and 400 mg/kg b.w. once in a day for 14 days respectively along with the single dose of cisplatin (7 mg/kg of body weight; i.p.) on day 1. The treatment duration was considered for 14 days as documented by Yang et.al. [12]

Urine was collected over 24 h on 14th day by keeping the test animals in individual metabolic cages. The volume of collected urine samples was measured followed by estimation of biochemical parameters, namely urine creatinine and urine albumin. Blood samples were collected from the test animals under anesthesia (phenobarbiton sodium; 40 mg/kg of body weight; i.p) by cardiac puncture before sacrifice and serum parameters including creatinine, urea, albumin, and total protein were estimated. [13],[14] The biochemical estimations were done in a Biochemical-semi-auto analyzer (Ebra-chem-5-plus, V2. West Germany) by standard procedures using commercial kits (Ecolin: Merck specialties, India). The kidneys were removed from the rats before sacrifice and organs were fixed [15] using a formosal solution (10% v/v of formaldehyde in normal saline), embedded with paraffin wax followed by preparation of tissue sections using a microtome for histopathology study. [16]


Data obtained in the experiment were expressed in terms of mean ± SEM. Statistical significance of data was assessed by analysis of variance (one-way ANOVA) followed by a comparison between different groups using "Tukey-Kramer" multiple comparison test. The significance level was set at P<0.05. The toxic control group was compared with the normal control group and all other treatment groups were compared with the toxic control group.

 » Results Top

The results as cited in [Table 1] includes change in body weight, kidney weight, urine volume along with the urine, and serum biochemistry data. Cisplatin administration-induced renal injury was prominent as evidenced by significantly depressed renal functions, body weight, and urine volume as compared to the normal control group.
Table 1: Parameters studied for the nephroprotective effect of the ethanol extract of Bauhinia variegata

Click here to view

The EEBV 400 group (ethanolic extract 400 mg/kg treated rat group) showed significant (P<0.001) elevation in body weight (7.16 ± 1.10) with a significant (P<0.05) increase in urine volume output (11.95 ± 0.79). However, the urine creatinine (1.81 ± 0.32) and albumin (0.31 ± 0.06) decreased significantly (P<0.01) as compared with the toxic control group. The serum creatinine (0.65 ± 0.09) and urea (32.86 ± 5.88) were found to be significantly (P<0.001) low when compared with the toxic control group.

The histological features found from the tissue sections of different groups are mentioned in [Table 2] and the photomicrographs of tissue sections are presented in [Figure 1]a--d. The histopathology of tissue sections suggest that the toxic control group had encountered vast histological damages as evidenced by the glomerular and tubular congestion with abnormal Bowman's capsule, blood vessel congestion, epithelial cell desquamation, and presence of tubular cast with few inflamatory cells. The histological features of the EEBV 400 group showed minimal cellular damage in contrast to the toxic control group. The EEBV 400 group showed normal glomerular and tubular arrangements with normal Bowmen's capsule. Congestion of blood vessels was minimal and tubular cast were not present.
Table 2: Histological features found from L.S of kidneys of different groups

Click here to view
Figure 1: Photomicrographas of L.S. of kidney of different groups (a) Normal Control Group, (b) Toxic Control Group, (c) Standard Group, (d) EEBV400 Group.

Click here to view

 » Discussion Top

The present study aimed to evaluate the protective effect of whole stem extract (ethanol) of BV Linn. plant against cisplatin-induced nephropathy in rats. Cisplatin-administered rats (toxic control group) had encountered acute kidney dysfunction as evidenced by elevation in serum urea and creatinine, decreased urine output and body weight with multiple histological damages. Treatment with the ethanol extract of BV at the dose level of 400 mg/kg b.w. for 14 days (EEBV400 group) significantly lowered the serum level of creatinine and urea, decreased urine creatinine and albumin with a significant weight gain, and increased urine output when compared with the toxic group. The histological damages in the BV extract-treated group were minimal in contrast to the toxic rats. The statistical significance of the nephroprotective activity of BV-treated group and the polyherbal drug cystone (standard group)-treated group (both the groups were compared against toxic control) were found almost equal as both groups gained same level of significance (P<0.001) against the toxic group in most of the parameters including serum urea and creatinine.

The results of our study suggest that the ethanolic extract of BV possesses nephroprotective potential depending on the dose levels. Extensive and multidimensional further research is needed to elucidate the exact mechanism of nephroprotective action of this plant extract.


The authors are thankful to Head of the Dept., University Department of Pharmaceutical Sciences, Utkal University, Bhubaneswra, Orissa for providing laboratory facilities to carryout this research work.

 » References Top

1.Zhang JG, Lindup WE. Role of mitochondria in Cisplatin-induced oxidative damage exhibited by rat renal cortical slices. Biochem Pharmacol 1993;45:2215-22.  Back to cited text no. 1
2.Chopra RN, Nayer SL, Chopra IC. Glossary of Indian Medicinal Plants. India: Council of Industrial and Scientific Research (CSIR); 1956. p. 35.  Back to cited text no. 2
3.Kirtikar KR, Basu BD. Indian Medicinal Plant. Dehradun: International Book Distributor; 1999. p. 892-901.  Back to cited text no. 3
4.The Ayurvedic Pharmacopoeia of India. Ministry of Health and family Welfare, Government of India. 1990. p. 10.  Back to cited text no. 4
5.Chanda S, Parekh J, Karathia NN. Evaluation of antibacterial activity and phytochemical analysis of Bauhinia variegata L. bark. Afr J Biomed Res 2006;9:53-6.  Back to cited text no. 5
6.Mali RG, Mahajan SG, Mehta AA. Rakta Kanchan (Bauhinia variegata): Chemistry, Traditional and Medicinal uses- a review. Pharmacogn Rev 2007;1:314-19.  Back to cited text no. 6
7.Gupta AK, Vidyapati TJ, Chauhan JS. Chemical examination of the stem of Bauhinia variegata. Planta Med 1980;38:174-6.  Back to cited text no. 7
8.Suftness M, Douras J. Drugs of plant origin -methods in cancer research. New York: Academic Press; 1979. p. 548.  Back to cited text no. 8
9.OECD. Acute oral toxicity- Acute oral toxic class method, Gudieline 423. adopted on 23.03.1996. In 11 th addendum to the OECD guidelines for testing of chemicals. Organization for economic co-operation and development. Paris, 2000.  Back to cited text no. 9
10.Suzuki CA, Cherian MG. Interaction of cis-diaminedichloroplatinum with metallothionein and glutathione in rat liver and kidney. Toxicology 1990;64:113-27.  Back to cited text no. 10
11.Rao M, Rao MN. Protective effect of Cystone, a polyherbal Ayurvedic Preparation on Cisplatin induced Renal toxicity in Rats. J Ethnopharmacol 1998;62:1-6.  Back to cited text no. 11
12.Yang HK, Yong WK, Young JO, Nam IB, Sun AC, Hae GC, et al. Protective effect of the ethanol extract of the roots of Brassica rapa on cisplatin-induced nephrotoxicity in LLC-PK1 cells and rats. Biol Pharm Bull 2006;29:2436-41.  Back to cited text no. 12
13.Cheesbrough M. District Laboratory Practice in Tropical Countries. England: Cambridge University Press; 2003. p. 310-95.  Back to cited text no. 13
14.Tietz. Text book of Clinical Chemistry. 3 rd ed. Phailadelphia: W.B Saunders Company; 1999. p. 617-721.  Back to cited text no. 14
15.Sood R. Medical Laboratory Technology-Methods and Interpretation. 4 th ed. India: Jaypee Bros Publications; 2002. p. 224-6.  Back to cited text no. 15
16.Ogeturk M, Kus I, Colakoglu N, Zararsiz I, Ilhan N, Sarsilmaz M. Caffeic acid phenethyl ester protects kidneys against CCl 4 toxcicity in rats. J Ethnopharmacol 2005;97:273-80.  Back to cited text no. 16


  [Figure 1]

  [Table 1], [Table 2]

This article has been cited by
1 Anti-Urolithiatic and Nephroprotective Activity of Quercetin and Betulin in Conjunction with a Bio Enhancer – An in Vivo Study
Shilpika Nagula, N. J. P. Subhashini, D. V. R. N. Bhikshapathi, Palanati Mamatha, Sailaja Rao. P
Biomedical and Pharmacology Journal. 2023; 16(2): 847
[Pubmed] | [DOI]
2 Three New Compounds Isolated from Bauhinia variegata Plant
Bushra, Hamid Ullah, Dilfaraz Khan, Fazal Ur Rehman, Nisar Ahmad, Shafiullah Khan
Pharmaceutical Chemistry Journal. 2021; 55(1): 76
[Pubmed] | [DOI]
3 Bauhinia variegata: a comprehensive review on bioactive compounds, health benefits and utilization
Kartik Sharma, Vikas Kumar, Satish Kumar, Rakesh Sharma, C. M. Mehta
Advances in Traditional Medicine. 2021; 21(4): 645
[Pubmed] | [DOI]
4 Protective effects of medicinal plant against diabetes induced cardiac disorder: A review
Sadegh Shabab, Zahra Gholamnezhad, Maryam Mahmoudabady
Journal of Ethnopharmacology. 2021; 265: 113328
[Pubmed] | [DOI]
5 Zingerone ameliorates cisplatin-induced ovarian and uterine toxicity via suppression of sex hormone imbalances, oxidative stress, inflammation and apoptosis in female wistar rats
Erdal kaygusuzoglu, Cuneyt Caglayan, Fatih Mehmet Kandemir, Serkan Yildirim, Sefa Kucukler, Mehmet Akif Kilinc, Yavuz Selim Saglam
Biomedicine & Pharmacotherapy. 2018; 102: 517
[Pubmed] | [DOI]
6 Effect of an extract of Bauhinia variegata leaves on chronic arsenic intoxication in mice (Mus musculus): A preliminary study
Surjyo Jyoti Biswas,Goutam Ghosh
TANG. 2014; 4(3): 20.1
[Pubmed] | [DOI]


Print this article  Email this article


Site Map | Home | Contact Us | Feedback | Copyright and Disclaimer | Privacy Notice
Online since 20th July '04
Published by Wolters Kluwer - Medknow