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| RESEARCH ARTICLE
|Year : 2010 | Volume
| Issue : 1 | Page : 21-26
Immunosuppressive properties of Pluchea lanceolata leaves
DP Bhagwat1, MD Kharya2, Sarang Bani3, Anpurna Kaul3, Kiranjeet Kour3, Prashant Singh Chauhan3, KA Suri4, NK Satti4
1 Pharmacology Research Laboratory, ASBASJSM College of Pharmacy, Bela (Ropar), Punjab, India
2 Department of Pharmaceutical Sciences, Dr. Hari Singh Gour University, Sagar, MP, India
3 Cell Biology Laboratory,Division of Pharmacology, Jammu Tawi, J and K, India
4 Natural Products Chemistry Division, Indian Institute of Integrative Medicine, Jammu Tawi, J and K, India
Objective : To investigate the immunosuppressive potential of Pluchea lanceolata 50% ethanolic extract (PL) and its bioactive chloroform fraction (PLC).
Materials and Methods : Preliminary screening of the Pluchea lanceolata 50% ethanolic extract (PL) was carried out with basic models of immunomodulation, such as, the humoral antibody response (hemagglutination antibody titers), cell-mediated immune response (delayed-type hypersensitivity), skin allograft rejection test, in vitro (C. albicans method), and in vivo phagocytosis (carbon clearance test).
The extract was then fractionated with chloroform, n-butanol, and water to receive the respective fractions by partitioning. These fractions were employed for flow cytometry to study the T-cell specific immunosuppressive potential of these fractions.
Results : Oral administration of PL at doses of 50 to 800 mg/kg in mice, with sheep red blood cells (SRBC) as an antigen, inhibited both humoral and cell-mediated immune responses, as evidenced by the production of the circulating antibody titer and delayed-type hypersensitiviy reaction results, respectively, and the immune suppression was statistically significant (P < 0.01) in Balb/C mice. PL also decreased the process of phagocytosis both in vitro (31.23%) and ex vivo (32.81%) and delayed the graft rejection time (30.76%). To study the T-cell-specific activities, chloroform, n-butanol, and water fractions from
P. lanceolata were tested for T-cell specific immunosuppressive evaluation, wherein only the chloroform fraction (PLC) showed significant (P < 0.01) suppression of CD8+ / CD4+ T-cell surface markers and intracellular Th1 (IL-2 and IFN- Y ) cytokines at 25 - 200 mg/kg p.o. doses. PLC, however, did not show significant suppression of the Th2 (IL-4) cytokine.
Conclusion : The findings from the present investigation reveal that P. lanceolata causes immunosuppression by inhibiting Th1 cytokines.
D P Bhagwat
Pharmacology Research Laboratory, ASBASJSM College of Pharmacy, Bela (Ropar), Punjab
Source of Support: None, Conflict of Interest: None
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