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Year : 2009  |  Volume : 41  |  Issue : 2  |  Page : 87-88

Studies on anti-inflammatory effect of aqueous extract of leaves of Holoptelea integrifolia, Planch. in rats

Department of Pharmacology, SRM College of Pharmacy, SRM University, SRM Nagar, Kattankulathur - 603 203, Tamil Nadu, India

Date of Submission19-Feb-2008
Date of Decision30-Jul-2008
Date of Acceptance30-Mar-2009

Correspondence Address:
Shrinivas Sharma
Department of Pharmacology, SRM College of Pharmacy, SRM University, SRM Nagar, Kattankulathur - 603 203, Tamil Nadu
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0253-7613.51348

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 » Abstract 

Objectives: The purpose of the present study was to investigate the anti-inflammatory properties of aqueous extract of the leaves of H. integrifolia, Planch.
Materials and Methods: The hind paw edema was produced in rats by subplanter injection of carageenan. The aqueous extract of H. integrifolia, Planch. (AHI) at dose (250 and 500 mg/kg p.o) was given to observe % inhibition of paw edema which were comparable with indomethacin (10 mg/kg p.o) used as a reference drug.
Results: The extract administered orally at doses of 250 and 500 mglkg p.o produced a significant (P < 0.05) dose dependent inhibition of edema formation
Conclusions: A significant % inhibition of paw edema by the aqueous extract of leaves of H. integrifolia, Planch. and its almost nearby same % inhibition with indomethacin suggest its usefulness as an anti-inflammatory agent.

Keywords: Antiinflammatory activity, Holoptelea integrifolia edema

How to cite this article:
Sharma S, Lakshmi K S, Patidar A, Chaudhary A, Dhaker S. Studies on anti-inflammatory effect of aqueous extract of leaves of Holoptelea integrifolia, Planch. in rats. Indian J Pharmacol 2009;41:87-8

How to cite this URL:
Sharma S, Lakshmi K S, Patidar A, Chaudhary A, Dhaker S. Studies on anti-inflammatory effect of aqueous extract of leaves of Holoptelea integrifolia, Planch. in rats. Indian J Pharmacol [serial online] 2009 [cited 2023 Mar 21];41:87-8. Available from: https://www.ijp-online.com/text.asp?2009/41/2/87/51348

Acute and chronic inflammatory diseases are still one of the most important health problems in the world. Although several agents are known to treat inflammatory disorders, their prolonged use often leads to gastric intolerance, bone marrow depression, water and salt retention. For this reason there is a need to find and develop new anti-inflammatory drugs with low side effects. [1]

Holoptelea integrifolia , Planch. belongs to the family of Urticaceae. It is an important pollen allergen of India and sensitizes almost 10% of the atopic population in Delhi. [2] Some recent explorations have been reported on this plant in which antiviral activity, [3] antioxidant, antimicrobial and wound healing activities [4] are important. Ethnomedically, the leaves and stem bark of this plant were used by local people for skin diseases, obesity, [5] cancer [6] and for wound healing in the form of paste. The fresh material, either stem bark or leaves of the plant, is applied as paste externally twice or thrice a day for wound-healing. The process of wound-healing involves inflammation, cell proliferation and contraction of collagen lattice formation. [7] Hence, the present study was under taken to evaluate the anti-inflammatory activity of aqueous extract of Holoptelea integrifolia, Planch. (AHI).

The leaves of H. integrifolia , Planch. were collected from the foothills of Yercaud, Tamil Nadu, India. The air-dried and powdered leaves of H. integrifolia , Planch. (400gm) were successfully extracted with petroleum ether by continuous hot percolation method using Soxhlet apparatus, the extract obtained was made free of any solvent by distillation and then aqueous extract was prepared by cold maceration procedure with distilled water.

The experimental protocol was approved by the Institutional Animal Care and Use Committee (IACUC) of the SRM University, Kattankulathur, Tamil Nadu, India. The anti-inflammatory activity was evaluated by the carrageenan-induced paw edema test in the male Wistar strain rats, [8] weighing between 150 and 200 gm. The animals were housed in standard isolation cages (45 × 35 × 25cm) under environmentally controlled conditions with a 12-h light/12-h dark cycle. Rats were allowed free access to water and standard laboratory rat chow (Hindustan Lever Pvt. Ltd, Mumbai).

Male Wistar rats group I (control) were treated with distilled water (10ml/kg), used as vehicle. Groups II and III were treated orally with AHI 250 and 500mg/kg, respectively, while group IV was treated orally with 10mg/kg of indomethacin used as reference, 30 min before 0.1ml 1% carrageenan in isotonic saline was injected subplantarly into right hind paws. The contralateral paw was injected with 0.1ml saline and used as a control. Measurements of paw volume (ml) were made by mercury displacing techniques using plethysmometer. Immediately before and 1, 2, 3 and 4h after carrageenan injection, percentage increment of paw volume after 1, 2, 3 and 4h was calculated by Newbould method. [9] The results are expressed as the mean ± S.E. Mean and the statistical significance of differences between groups was analyzed by one-way analysis of variance (ANOVA). P < 0.05 was considered as significant.

The subplantar injection of carrageenan caused a time-dependent paw edema in the rat although saline injection caused no swelling (data not shown). In carrageenan-induced paw edema in rats, oral administration of AHI (250 and 500mg/kg p.o.) inhibited paw swelling dose-dependently at 2, 3, and 4hr after carrageenan injection ( P < 0.05) which was comparable with the indomethacin treated group. Percent increment in paw swelling was calculated using the values before carrageenan injection [Figure 1].

The present data showed that the aqueous extract of leaves of Holoptelea integrifolia , Planch. exhibits anti-inflammatory activity. Further studies are in progress in our laboratory to discover which constituent of the extract exerts this activity and to explore its exact mechanism of action.

 » References Top

1.Saxena RS, Gupta B, Saxena KK, Singh R, Prasad DN. Study of Anti-inflammatory Activity of the leaves of Nyctanthes arbor-tristis Linn.: An Indian Medicinal Plant. J Ethnopharmacol 1987;11:319-30.  Back to cited text no. 1    
2.Sharma S, Panzani RC, Gaur SN, Ariano R, Singh AB. Evaluation of cross-reactivity between Holoptelea integrifolia and Parietaria judaica. Int Arch Allergy Immunol 2005;136:103-12.  Back to cited text no. 2  [PUBMED]  [FULLTEXT]
3.Rajbhandari M, Wegner U, Julich M, Schopke T, Mentel R. Screening of Nepalese medicinal plants for antiviral activity. J Ethnopharmacol 2001;252:251-5.  Back to cited text no. 3    
4.Chandrasekar D, Madhusudhana K, Ramakrishna S, Diwan PV. Evaluation of antimicrobial, antioxidant and wound-healing potentials of Holoptelea integrifolia. J Ethnopharmacol 2008;115:249-56.  Back to cited text no. 4    
5.Bambhole VB, Jiddewar GG. Anti-obesity effect of Iris versicolor and Holoptelea integrifolia in rats. Sachitra Ayurved 1985;37:557- 61.  Back to cited text no. 5    
6.Graham JG, Quinn ML, Fabricant DS, Farnsworth NR. Plants used against cancer: An extension of the work of Jonathan Hartwell. J Ethnopharmacol 2000;73: 347-77.  Back to cited text no. 6  [PUBMED]  [FULLTEXT]
7.Sidhu GS, Mani H, Gaddipatti, JP, Singh AK, Seth P, Banaudha KK, et al . Curcumin enhances wound healing in streptozotocin induced diabetic rats and genetically diabetic mice. Wound Repair Reg 1999;7:362-74.  Back to cited text no. 7    
8.Winter CA, Risley EA, Nuss GW. Carrageenan-induced edema in hind paw of rat as an assay for antiinflammatory drugs. Pro Soc Biol Med 1962;11:544-7.  Back to cited text no. 8    
9.Thomas CA, Rama Sarma GV. Analgesic and anti-inflammatory activities of Melia dubia bark. Indian Drugs 1999;6:203-5.  Back to cited text no. 9    


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