IPSIndian Journal of Pharmacology
Home  IPS  Feedback Subscribe Top cited articles Login 
Users Online : 10461 
Small font sizeDefault font sizeIncrease font size
Navigate Here
 » Next article
 » Previous article 
 » Table of Contents
Resource Links
 »  Similar in PUBMED
 »  Search Pubmed for
 »  Search in Google Scholar for
 »Related articles
 »  Article in PDF (98 KB)
 »  Citation Manager
 »  Access Statistics
 »  Reader Comments
 »  Email Alert *
 »  Add to My List *
* Registration required (free)

In This Article
 »  Abstract
 »  Materials and Me...
 »  Results
 »  Discussion
 »  Acknowledgements
 »  References
 »  Article Tables

 Article Access Statistics
    PDF Downloaded752    
    Comments [Add]    
    Cited by others 30    

Recommend this journal


Year : 2008  |  Volume : 40  |  Issue : 3  |  Page : 126-128

Evalution of anti-ulcer activity of Polyalthia longifolia (Sonn.) Thwaites in experimental animals

1 Rofel Shri GM Bilakhia College of Pharmacy, Vapi (West)-396 191, Gujarat State, India
2 R and D Leader, Schering Plough Link, Singapore Branch, Tuas West drive, Singapore 638 403, Singapore
3 Asthagiri Herbal Research foundation, Tambaram Sanatorium, Chennai-600 053, Tamil Nadu, India

Date of Submission30-Aug-2007
Date of Decision14-Jan-2008
Date of Acceptance21-Jun-2008

Correspondence Address:
P Malairajan
Rofel Shri GM Bilakhia College of Pharmacy, Vapi (West)-396 191, Gujarat State
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0253-7613.42306

Rights and Permissions

 » Abstract 

Objective: To evaluate the anti-ulcer activity of ethanol extract of leaves of Polyalthia longifolia (Sonn.) Thwaites.
Materials and Methods: The ethanol extract of Polyalthia longifolia was investigated for its anti-ulcer activity against aspirin plus pylorous ligation induced gastric ulcer in rats, HCl -Ethanol induced ulcer in mice and water immersion stress induced ulcer in rats at 300 mg/kg body weight.p.o.
Results: A significant (P <0.01, P <0.001) anti ulcer activity was observed in all the models. Pylorous ligation showed significant (P<0.01) reduction in gastric volume, free acidity and ulcer index as compared to control. It also showed 89.71% ulcer inhibition in HCl- Ethanol induced ulcer and 95.3% ulcer protection index in stress induced ulcer.
Conclusion: This present study indicates that P. longifolia leaves extract have potential anti ulcer activity in the three models tested.

Keywords: Aspirin pylorous ligation ulceration, HCl-Ethanol ulcer, Polyalthia longifolia, stress ulcer

How to cite this article:
Malairajan P, Gopalakrishnan G, Narasimhan S, Veni K J. Evalution of anti-ulcer activity of Polyalthia longifolia (Sonn.) Thwaites in experimental animals. Indian J Pharmacol 2008;40:126-8

How to cite this URL:
Malairajan P, Gopalakrishnan G, Narasimhan S, Veni K J. Evalution of anti-ulcer activity of Polyalthia longifolia (Sonn.) Thwaites in experimental animals. Indian J Pharmacol [serial online] 2008 [cited 2023 Oct 4];40:126-8. Available from: https://www.ijp-online.com/text.asp?2008/40/3/126/42306

In traditional medicines various herbal preparations are being used for treating duodenal ulcers. [1] Polyalthia longifolia (Sonn.) Thwaites (PL) (Family: Annonaceae) is a tall handsome evergreen tree and it is cultivated all over India. The plant has been used in traditional system of medicine for the treatment of fever, skin diseases, diabetes, hypertension and helminthiasis. [2] A number of biologically active compounds have been isolated from this plant. [3],[4],[5],[6] The plant extract and isolated compounds were studied for various biological activities like antibacterial activity, cytotoxicity, antifungal activity. [7],[8],[9],[10],[11] The present study was undertaken to evaluate the anti ulcer activity of ethanol extract of P. longifolia (PL) against aspirin plus pylorous ligation induced gastric ulcer, HCl - Ethanol induced ulcer and water immersion stress induced ulcer in rats.

 » Materials and Methods Top

Plant material

The leaves of Polyalthia longifolia was collected from Kancheepuram Dist, South India during September 2000 and authenticated by Professor P. Jeyaraman, Plant Anatomy Research Centre, Tambaram, Chennai-45. The specimen voucher was deposited in the Asthagiri Herbal Research Foundation [AHRF 09] Chennai-59.

The leaves of P. longifolia were air-dried and powdered plant material was extracted by maceration with ethanol for 72h. The extract was concentrated using rotary vacuum to get the solid mass. The yield obtained was 13.73%. The extract, ranitidine, sucralfate and omeprazole were suspended in 1% sodium carboxy methyl cellulose (SCMC) and used for anti-ulcer studies.


albino rats of both sex and Swiss albino mice were obtained from Tamil Nadu Veterinary College and Research Institute, Chennai. The animals were housed in polypropylene cages at 24±2°C and fed with commercial pellet diet and water ad libitum. All animal experiments were carried out in accordance with the guidelines of CPCSEA and the study was approved by the Institutional Animal Ethics Committee.

Acute oral toxicity studies

A safe oral dose of the extract was determined by acute oral toxic class method of Organization of Economic Co-Operation and Development (OECD) as per 423 guidelines. [12] The plant reported to contain the antiulcerogenic principle liriodenine and annonains. So the present study followed three approaches of antiulcerogenic mechanism of the plant extract:

  1. Aspirin plus pylorus ligation induced gastric ulcer in rats (antisecretory mechanism).
  2. HCl - Ethanol induced ulcer (cytoprotective mechanism).
  3. Water immersion stress induced ulcer in rats (proton pump inhibition mechanism).

For each model the corresponding standard drug such as ranitidine, sucralfate and omeprazole was used.

Aspirin plus pylorus ligation induced gastric ulcer in rats

The Wistar albino rats weighing 100-200 g of either sex were divided into 3 groups, each group consists of 6 animals. All the animals received 200 mg/kg of aspirin once daily for three days . Group 1 served as control received 1.0 ml/kg p.o 1% SCMC, group 2 treated with 50 mg/kg, p.o ranitidine as standard, group 3 treated with 300 mg/kg, p.o. ethanol extract of PL. On the fourth day pylorus part was ligated following 36h fasting. [13] Four hours after the pyloric ligation the animals were sacrificed by decapitation. The stomach was opened and the ulcer index was determined. [14] The gastric content was titrated against 0.01 N NaOH to find out the free acidity and total acidity. [15]

Ulcer lesion Index method: HCl - Ethanol induced ulcer

Swiss albino mice weighing 24-30 g of either sex were divided into 3 groups, each group consists of 6 animals. Group 1 served as a control received 1.0 ml/kg p.o 1% SCMC, group 2 received 100 mg/kg, p.o sucralfate as standard control; group 3 received 300 mg/kg, p.o ethanol extract of PL. After 1h all the animals were treated with 0.2 ml of HCl - Ethanol mixture p.o (0.3 M Hydrochloric acid and ethanol 60%) to induce gastric ulcer. Animals were sacrificed by cervical dislocation one hour after administration of HCl - Ethanol mixture. The stomach was excised and lesion index was determined by measuring each lesion in mm along its greater length. [16]

Water immersion stress induced ulcer in rats

Stress ulcers were induced by forced swimming in the glass cylinder [17] (height 45 cm, diameter 25 cm) containing water to the height of 35 cm maintained at 25°C for 3h. Animals were fasted for 24h prior to the experiment and divided in to 3 groups with 6 animals in each group. Group 1 received 1.0 ml/kg p.o 1% SCMC as vehicle control, group 2 treated with 20 mg/kg, p.o. omeprazole as standard control, group 3 received 300 mg/kg, p.o ethanol extract of PL. After the drug treatment animals were allowed to swim in water for 3 h. The stomach of each animal was removed and the extent of gastric damage was assessed as described by Alphine and Word. [18]

Statistical analysis

The statistical analysis of all the results was carried out using one-way ANOVA followed by Dunnets multiple comparisons using graph pad in stat 3 and all the results obtained in the study were compared with the vehicle control group.

 » Results Top

In aspirin plus pylorous ligation induced gastric ulcer the ethanol extract of PL showed significant (P<0.01) reduction in gastric volume, free acidity and ulcer score (% ulcer inhibition 53.69) as compared to control [Table 1]. From [Table 2] it can be observed that the number of lesions in HCI-Ethanol induced peptic ulcer group was significantly high and the ethanol extract of PL pretreated group depicted marked reduction (P <0.01) in gastric lesion (% ulcer inhibition 89.71) as compared to control [Table 2].

In water immersion stress induced ulcer the mean score value of ulcer inhibition was found to be significant (P <0.001) and the % ulcer inhibition was 95.3 [Table 3].

 » Discussion Top

In aspirin plus pylorus ligation induced gastric ulcer model the ethanol extracts of P. longifolia reduced the gastric volume, free acidity, total acidity and ulcer index thus showing the anti-secretory mechanism involved in the extracts for their anti-ulcerogenic activity. Ulcer index parameter was used for the evaluation of anti-ulcer activity since ulcer formation is directly related to factors such as gastric volume, free and total acidity. In case of vehicle control, aspirin plus pylorus ligation increased the acid secretion, which in turn caused increase in gastric volume, low pH, increased free and total acidity resulting into increase in ulcer index. [19]

HCl-Ethanol induced gastric damage is possibly through leukotrienes production and 5-lipooxygenase pathway. Prostaglandins also play a role in ethanol-induced ulcer. It has been shown that drugs which are effective against HCI-Ethanol induced gastric lesions can possess gastric mucosal membrane protective action. The protective effect of the extract P. longifolia against the gastric damage may be due to their action against 5-lipooxygenase pathway. The cytoprotective action probably stimulates the prostaglandin synthesis, which in turn protects the gastric mucosa.

Water immersion stress is one of the best models of stress in rats to induce ulcer. The model provides both emotional stress as well as physiological stress to the animal. In case of water immersion induced stress in rats, the extract showed significant (P<0.001) ulcer inhibition. Preliminary phytochemical investigations showed the presence of alkaloids and terpenoids, hence the antiulcer activity of PL in this experimental model may be due to the above mentioned chemicals. The results indicate that P. longifolia extract produced antiulcerogenic effects possessing antisecretory, cytoprotective and proton pump inhibition mechanism. This study indicates that P. longifolia extract has a potertial anti ulcer activity. However, further study is required to isolate the active molecule responsible for the activity.

 » Acknowledgements Top

Authors thank Professor P. Jeyaraman, Plant Anatomy Research Centre, Tambaram, Chennai for providing authentic plant materials.

 » References Top

1.Chatterjee TK. Herbal options. 3rd ed. Calcutta: Books and Allied (p) Ltd; 2000. p. 203-56.  Back to cited text no. 1    
2.Kirtikar KR, Basu BD. Indian medicinal plants. Dehradhun: International Book Distributors; 1995. p. 562.  Back to cited text no. 2    
3.Wu YC, Duh CY, Wang SK, Chen KS, Yang TH. Two new natural azofluorene alkaloids and a cytotoxic aporphine alkaloids from P. longifolia. J Nat Prod 1990;53:1327-31.  Back to cited text no. 3    
4.Zhao GX, Jung JH, Smith DL, Wood KV, McLaughlin JL. Cytotoxic clerodane from P. longifolia. Planta Med 1991;57:380-3.  Back to cited text no. 4    
5.Chakrabarty M, Amarnath C. A new clerodane type butenolide diterpene from the bark of P. longifolia. J Nat Prod 1992;55:256-8.  Back to cited text no. 5    
6.Hara N, Asaki H, Fujimoto Y, Gupta YK, Singh AK, Sahai M. Clerodane and ent-halamane diterpene from Polyalthia longifolia. Phytochemistry 1995;38:189-94.  Back to cited text no. 6    
7.Marthanda Murthy M, Subramaniyam M, Hima Bindu M, Annapurna J. Antimicrobial activity of clerodane diterpenoids from Polyalthia longifolia seeds. Fitoterapia 2005;76:336-9.  Back to cited text no. 7    
8.Stιvigny C, Bailly C, Quetin-Leclercq J. Cytotoxic and antitumor potentialities of Aporphinoid alkaloids. Curr Med Chem Anticancer Agents 2005;5:173-82.  Back to cited text no. 8    
9.Nair R, Chanda S. Evaluation of Polyalthia longifolia (Sonn.) Thw.leaf extract for antifungal activity. J Cell Tissue Res 2006;6:581-4.  Back to cited text no. 9    
10.Nair R, Chanda S. Activity of some medicinal plants against certain pathogenic bacterial strains. Indian J Pharmacol 2006;38:142-4.  Back to cited text no. 10    
11.Jain AK, Jain A, Jain S, Sikarwar MS, Dubey SK. Hepatoprotective activity of ethanolic extract of leaves of Polyalthia longifolia. Plant Arch 2006;6:841-2.  Back to cited text no. 11    
12.Ecobichon DJ. The basis of toxicity testing. New York: CRC Press; 1997. p. 43-9.  Back to cited text no. 12    
13.Shay H, Komarov SA, Fels SE, Meraze D, Gruenstein M, Siplet H. A simple method for the uniform production of gastric ulceration. Gastroenterology 1945;5:43-61.  Back to cited text no. 13    
14.Ganguly AK, Bhatnagar OP. Effect of bilateral adrenalatomy on production of restraint ulcers in the stomach of albino rats. Can J Physiol Pharmacol 1973;51:748-50.  Back to cited text no. 14    
15.Kulkarni SK. "Handbook of Experimental Pharmacology," 3rd ed. New Delhi: Vallabh Prakashan; 1999. p. 148-50.  Back to cited text no. 15    
16.Yesilada E, Gurbutz I, Ergun E. Effect of Cistus laurifolius L. flowers on gastric and duodenal lesions. J Ethnopharmacol 1997;55:201-11.  Back to cited text no. 16    
17.Alder R. In Breakdown in human adaptation to stress. Boston: Martinus Ninjihoff; 1984. p. 653.  Back to cited text no. 17    
18.Alphine RS, Word JW. Antihistaminic activity and ulceration. Eur J Pharmacol 1969;6:61-6.  Back to cited text no. 18    
19.Goel RK, Bhattacharya SK. Gastro duodenal mucosal defense and mucosal protective agents. Indian J Expl Biol 1991;29:701-14.  Back to cited text no. 19    


  [Table 1], [Table 2], [Table 3]

This article has been cited by
1 Metabolomic profiling and bactericidal effect of Polyalthia longifolia (Sonn.) Twaites. stem bark against methicillin-resistant Staphylococcus aureus
Gael Tchokomeni Siwe, Marguerite Kamdem Simo, Rukesh Maharjan, Andre Perfusion Amang, Christophe Mezui, Paul Vernyuy Tan
Access Microbiology . 2023; 5(6)
[Pubmed] | [DOI]
2 A Review on Traditional Anti-ulcer Medicinal Plants
Ambrish K. Singh, Priyamvada Arun, Jyoti Shukla, Ajay Kumar, Manmath K. Nandi, Zainularifeen Abduljaleel, Saeed S. Al-Ghamdi, Abdullah R. Alzahrani, Naiyer Shahzad
Letters in Drug Design & Discovery. 2023; 20(4): 408
[Pubmed] | [DOI]
3 Ethnomedicinal and Ethnobotanical Investigations and Documentation of Plants Used by Traditional Healers of Eastern India
Melaku Masresha Woldeamanel, Soumyaranjan Senapati, Shibani Mohapatra, Hatanath Subudhi, Prasanta Rath, Alok Kumar Panda
Current Traditional Medicine. 2022; 8(6)
[Pubmed] | [DOI]
4 Review on Peptic ulcer and its effective Management and Treatment with Herbals
Manisha Bhatti, Divya Dhawal Bhandari, Jitender Singh
Research Journal of Pharmacy and Technology. 2022; : 3580
[Pubmed] | [DOI]
5 Preliminary Study of Gastroprotective Effect of Aloe perryi and Date Palm Extracts on Pyloric Ligation-Induced Gastric Ulcer in Experimental Rats
Naif Al-Gabri, Gehad M. Elnagar, Sultan A. M. Saghir, Amina El-Shaibany, Sultan F. Alnomasy, Ziyad M. Althafar, Nesreen M. I. M. Elkomy, Mahmoud M. Elaasser, Maisa Siddiq Abdoh, Mohammed Yosri, Ahmet Özer Sehirli
BioMed Research International. 2022; 2022: 1
[Pubmed] | [DOI]
6 Polyalthia longifolia: phytochemistry, ethnomedicinal importance, nutritive value, and pharmacological activities review
Sayeed Mohammed Firdous, Sk Nasim Ahmed, Sk Maruf Hossain, Soumya Ganguli, Marwa A. A. Fayed
Medicinal Chemistry Research. 2022;
[Pubmed] | [DOI]
7 Lead phytomolecules for gastroprotective drug development
Yogesh Murti, Krishn Kumar Agrawal, Bhupesh Chander Semwal, Sonia Singh
Advances in Traditional Medicine. 2022;
[Pubmed] | [DOI]
8 Traditional Herbal Medicines Against CNS Disorders from Bangladesh
Md. Josim Uddin, Christian Zidorn
Natural Products and Bioprospecting. 2020; 10(6): 377
[Pubmed] | [DOI]
9 Influence of Polyalthia longifolia (Sonn) leaves on oxidative stress biomarkers in the kidney of cadmium-induced toxicity rats
Ajibade O. Oyeyemi, Olatunde A. Oseni, Olorunfemi R. Molehin, Adebimpe O. Babatunde
Comparative Clinical Pathology. 2020; 29(2): 525
[Pubmed] | [DOI]
10 Modulatory effect of Polyalthia longifolia leaves against cadmium-induced oxidative stress and hepatotoxicity in rats
Ajibade O. Oyeyemi, Olatunde A. Oseni, Adebimpe O. Babatunde, Olorunfemi R. Molehin
Journal of Complementary and Integrative Medicine. 2020; 17(4)
[Pubmed] | [DOI]
11 Medicinal Plants in the Treatment of Peptic Ulcer Disease: A Review
Hamidreza Ardalani, Amin Hadipanah, Amirhossein Sahebkar
Mini-Reviews in Medicinal Chemistry. 2020; 20(8): 662
[Pubmed] | [DOI]
12 Antihyperlipidemic Activities and Hematological Properties of Ethanol Extract of Blighia Sapida Koenig Bark in Alloxan-Induced Diabetic Rats
Oluwafemi Adeleke Ojo, Adebola Busola Ojo, Basiru Olaitan Ajiboye, Oluwatosin Debbie Imiere, Babatunji Emmanuel Oyinloye
Serbian Journal of Experimental and Clinical Research. 2020; 21(1): 11
[Pubmed] | [DOI]
13 Antioxidant and antityrosinase proanthocyanidins from Polyalthia longifolia leaves
Xiao-Xin Chen,Ge Liang,Wei-Ming Chai,Hui-Ling Feng,Han-Tao Zhou,Yan Shi,Qing-Xi Chen
Journal of Bioscience and Bioengineering. 2014;
[Pubmed] | [DOI]
14 A clerodane diterpene inhibit adipogenesis by cell cycle arrest and ameliorate obesity in C57BL/6 mice
Muheeb Beg,Kripa Shankar,Salil Varshney,Sujith Rajan,Suriya Pratap Singh,Pankaj Jagdale,Anju Puri,Bhushan P. Chaudhari,Koneni V. Sashidhara,Anil Nilkanth Gaikwad
Molecular and Cellular Endocrinology. 2014;
[Pubmed] | [DOI]
15 Natural products in treatment of ulcerative colitis and peptic ulcer
Amani S. Awaad,Reham M. El-Meligy,Gamal A. Soliman
Journal of Saudi Chemical Society. 2013; 17(1): 101
[Pubmed] | [DOI]
16 In vitro and in vivo antidiabetic activity of Polyalthia longifolia (Sonner.) Thw. leaves
Andichettiar Thirumalaisamy Sivashanmugam,Tapan Kumar Chatterjee
Oriental Pharmacy and Experimental Medicine. 2013;
[Pubmed] | [DOI]
17 Seasonal Abundance and Biology ofCrotonothrips polyalthiae(Thysanoptera: Phlaeothripidae) and Its Damage to a Shade Tree,Polyalthia longifolia
Andi Nasruddin,Laurence A. Mound
Florida Entomologist. 2012; 95(3): 610
[Pubmed] | [DOI]
18 Acute oral toxicity ofPolyalthia longifoliavar.pendulaleaf extract in Wistar albino rats
Sumitra Chanda,Rajeshkumar Dave,Mital Kaneria,Vinay Shukla
Pharmaceutical Biology. 2012; 50(11): 1408
[Pubmed] | [DOI]
19 Rapid synthesis of silver nanoparticles from Polylthia longifolia leaves
Tollamadugu Nagavenkata Krishina Va Prasad,Erusan Kuppan Elumalai,Shaik Khateeja
Asian Pacific Journal of Tropical Disease. 2012; 2: S663
[Pubmed] | [DOI]
20 Evaluation of Gastric and Duodenal Antiulcer Activity of Famotidine Formulation in Experimental Animals
S. Ramachandr, G. Poovi, M.D. Dhanaraju
Journal of Pharmacology and Toxicology. 2011; 6(2): 189
[VIEW] | [DOI]
21 In vitro screening of five hainan plants of Polyalthia (Annonaceae) against human cancer cell lines with MTT assay
Yuan, Y., Huang, G.-J., Wang, T.-S., Chen, G.-Y.
Journal of Medicinal Plant Research. 2011; 5(5): 837-841
22 Evaluation of antioxidant and anti-ulcerative potential of Citrullus lanatus seed extract in rats
Singh Gill, N., Sood, S., Muthuraman, A., Bali, M., Dev Sharma, P.
Latin American Journal of Pharmacy. 2011; 30(3): 429-434
23 The chemistry, pharmacologic, and therapeutic applications of Polyalthia longifolia
Katkar, K., Suthar, A., Chauhan, V.
Pharmacognosy Reviews. 2010; 4(7): 62-68
24 Effect of fucoidan on aspirin-induced stomach ulceration in rats
Choi, J.-i., Raghavendran, H.R.B., Sung, N.-Y., Kim, J.-H., Chun, B.S., Ahn, D.H., Choi, H.-S., Lee, J.-W.
Chemico-Biological Interactions. 2010; 183(1): 249-254
25 Pharmacognostic studies and physicochemical properties of the polyalthia longifolia var. pendula leaf
Dave, R., Nagani, K., Chanda, S.
Pharmacognosy Journal. 2010; 2(13): 572-576
26 Anti-ulcer activity of Caesalpinia bonducella (Linn.) Flem. leaves
Patil, K.S., Wadkar, G., Mathapati, S., Hogade, M., Karale, S., Deshpande, S.
Journal of Natural Remedies. 2010; 10(2): 91-96
27 Anti-ulcer effect of alcoholic extracts of Morus alba Linn. leaves in rodents
Hogade, M.G., Hariprassanna, R.C., Patil, K.S., Mathapati, S., Wadkar, G., Rao, K.P.
Indian Drugs. 2010; 47(6): 64-68
28 Effect of fucoidan on aspirin-induced stomach ulceration in rats
Jong-il Choi,Hanumatha Rao Balaji Raghavendran,Nak-Yun Sung,Jae-Hun Kim,Byeong Soo Chun,Dong Hyun Ahn,Hong-Seok Choi,Keon-Wook Kang,Ju-Woon Lee
Chemico-Biological Interactions. 2010; 183(1): 249
[Pubmed] | [DOI]
29 Pharmacognostic Studies and Physicochemical Properties of the Polyalthia longifolia var. pendula Leaf
Rajeshkumar Dave,Krunal Nagani,Sumitra Chanda
Pharmacognosy Journal. 2010; 2(13): 572
[Pubmed] | [DOI]
30 Evaluation of Antioxidant and Antiulcer Potential of Cucumis sativum L. Seed Extract in Rats
N.S. Gill, M. Garg, R. Bansal, S. Sood, A. Muthuraman, M. Bali, P.D. Sharma
Asian Journal of Clinical Nutrition. 2009; 1(3): 131
[VIEW] | [DOI]


Print this article  Email this article


Site Map | Home | Contact Us | Feedback | Copyright and Disclaimer | Privacy Notice
Online since 20th July '04
Published by Wolters Kluwer - Medknow