| RESEARCH ARTICLE
|Year : 2008 | Volume
| Issue : 2 | Page : 64-68
Paralytic effect of alcoholic extract of Allium sativum and Piper longum on liver amphistome, Gigantocotyle explanatum
TU Singh, D Kumar, SK Tandan
Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar - 243 122, UP, India
Objective: To investigate the effects of alcoholic extract of Allium sativum and Piper longum on the muscular activity of a parasitic amphistome, Gigantocotyle explanatum.
Materials and Methods: Amphistomes were isometrically mounted to record the spontaneous muscular activity by using Chart 4 software program (Power Lab, AD Instruments, Australia) and to examine the effects of cumulative doses (100, 300, 1000, and 3000 µg/ml) of the plant extracts on the amplitude (g), frequency (per 10 min), and baseline tension (g) of the spontaneous muscular activity of the amphistome.
Results: Alcoholic extract of A. sativum produced significant reduction in the frequency and amplitude of contractile activity of the amphistome at 1000 and 3000 µg/ml bath concentrations. Complete paralysis of the amphistome was observed after 15 min of addition of 3000 µg/ml concentration. Alcoholic extract of P. longum also caused paralysis following 15-20 min exposure of the amphistome to 3000 µg/ml concentration. In both the cases the amphistomes did not recover from paralysis following 2-3 washes.
Conclusion: The observations demonstrate the paralytic effect of alcoholic extract of A. sativum and P. longum on G. explanatum.
Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar - 243 122, UP
Source of Support: None, Conflict of Interest: None
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