IPSIndian Journal of Pharmacology
Home  IPS  Feedback Subscribe Top cited articles Login 
Users Online : 9 
Small font sizeDefault font sizeIncrease font size
Navigate Here
 »   Next article
 »   Previous article
 »   Table of Contents

Resource Links
 »   Similar in PUBMED
 »  Search Pubmed for
 »  Search in Google Scholar for
 »Related articles
 »   Citation Manager
 »   Access Statistics
 »   Reader Comments
 »   Email Alert *
 »   Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded139    
    Comments [Add]    
    Cited by others 3    

Recommend this journal


Year : 2004  |  Volume : 36  |  Issue : 2  |  Page : 87-92

Screening of certain chemoprotectants against cyclic peptide toxin microcystin-LR

Division of Pharmacology and Toxicology, Defence Research and Development Establishment, Jhansi Road, Gwalior - 474002, MP, India

Correspondence Address:
P V Lakshmana Rao
Division of Pharmacology and Toxicology, Defence Research and Development Establishment, Jhansi Road, Gwalior - 474002, MP
Login to access the Email id

Source of Support: None, Conflict of Interest: None

Rights and PermissionsRights and Permissions

Objective: To evaluate the protective efficacy of certain chemoprotectants against cyclic peptide hepatotoxic microcystin-LR in mice. Material and Methods: Swiss albino female mice were used in all experiments for screening antidotes against the lethal dose of microcystin-LR (100 mg/kg body weight, i.p.). The agents, D-glucose, mannitol, dihydroxyacetone, Trolox®, L-cysteine, N-acetylcysteine, amifostine, glutathione, silymarin, naringin, rifampin and cyclosporin-a were administered as either pre-treatment (1, 3 and 24 h), co-administration or post-treatment. Percent survival and time to death were monitored. The biochemical profile of protected animals was monitored 24 h post-treatment. Results: D-glucose, mannitol, L-cysteine, naringin and amifostine extended the survival time of animals but offered no protection against lethality. N-acetylcysteine, glutathione and Trolox® gave partial protection (25-50%) on pretreatment or co-administration. Complete protection was observed with rifampin (25 mg/kg), cyclosporin-A (10 mg/kg) and silymarin (400 mg/kg) when given as pre-treatment. In addition, rifampin and cyclosprin-A gave complete protection when co-administered with microcystin-LR. Rifampin was the only agent which gave protection at 15 min post-treatment. The biochemical profile of surviving animals 24 h after treatment showed increased liver body weight index and levels of hepatic enzymes viz. LDH, ALT, SDH in serum. Conclusion: Chemoprotectants that can inhibit the specific uptake of toxin into the liver can be promising antidotes against lethal poisoning by microcystin-LR in mice.


Print this article     Email this article

Site Map | Home | Contact Us | Feedback | Copyright and Disclaimer | Privacy Notice
Online since 20th July '04
Published by Wolters Kluwer - Medknow