| RESEARCH PAPER
|Year : 2000 | Volume
| Issue : 6 | Page : 361-364
Effect of HD-03 on levels of various enzymes in paracetamol-induced liver damage in rats
Udupa Venkatesha, KS Kulkarni, Rafiq Md., S Gopumadhavan, MV Venkataranganna, SK Mitra
Objective: To review the evidence for a role of HD-03, a polyherbal formulation in regulating the sodium pump in hepatic injury induced by paracetamol.
Methods: Alterations in sodium pump was induced by chronic administration of paracetamol at the dose of 500 and 1000 mg/kg, b. wt. for 28 days. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), liver glutathione, glycogen and Na+ -K+ ATPase activity estimation and histology of liver were studied in rats.
Results: Chronic administration of paracetamol for 4 weeks to rats produced dose dependent increase in ALT, AST and reduction in liver Na+ -K+ -ATPase activity, glycogen and glutathione levels, indicating the hepatocellular damage. Histological evaluation supported this change with evidence of swelling, hydropic degeneration and necrosis of the hepatocytes. These changes were reversed with simultaneous administration of paracetamol and HD-03 at 750 mg/kg, for 28 days.
Conclusion: Reversal of Na+ -K+-ATPase, glycogen, glutathione levels and restricted hepatic damage in HD-03 treated animals confirms the hepatoprotective effect of HD-03. Thus, Na+ -K+ -ATPase may be considered as a marker to evaluate the hepatoprotective effects of various herbs.
Source of Support: None, Conflict of Interest: None