| RESEARCH PAPER
|Year : 1997 | Volume
| Issue : 2 | Page : 105-109
Cell culture derived Hemidesmus indicus in the prevention of hypercholesterolemia in normal and hyperlipidemic rats
KN Bopanna, N Bhagyalakshmi, SP Rathod, R Balaraman, J Kannan
Objectives: To study the effect of cell culture extract of Hemidesmus indicus (CCH) on normal and hypercholesterolemic rats for various lipid profile in serum, tissues and fecal matter.
Methods: Twelve groups of eight animals were subgrouped into three set of experiments. First group received normal and CCH (2, 4 and 16 mg/kg, p.o.) for 30 days, second group received atherogenic diet for 30 (l-30) days and CCH (2, 4 and 16 mg/kg, p.o.) for next 30 (31-60) days, third group received atherogenic diet and CCH (2,4 and 16 mg/kg, p.o.) for 60 days concurrently. Serum tissue and faecal lipid profile were analysed.
Results: It was observed that the CCH (2, 4, and 16 mg/kg, p.o.) had prevented hypercholesterolemia in rats. Rats which received atherogenic diet for first 30 (l-30) days when subjected to CCH (2, 4 and 16 mg/kg, p.o.) for next 30 (31-60) days showed significant treatment reduction (p<0.01) of lipid levels in serum. When atherogenic diet and CCH were given together for 60 (l-60) days there was significant (p<0.01) reduction of lipid in liver, heart and serum fecal excretion of cholesterol and phospholipids were significantly increased (p<0.01 in rats fed with CCH.
Conclusion: Atherogenic diet along with CCH concurrently lowered the levels of serum, tissue and fecal lipid levels.
K N Bopanna
Source of Support: None, Conflict of Interest: None