| SHORT COMMUNICATION
|Year : 1989 | Volume
| Issue : 3 | Page : 104-106
Modification of histamine induced wheal suppression method and its utility in bioassay of topical steroids
PK Singh, G Singh
1. Histamine induced wheal suppression technique of Reddy and Singh (1976) has been modified by calculating volume of the wheal.
2. Height of the wheal was measured with the help of a spherometer to calculate its volume by the relation (r2h.
3. Potency of two topical corticosteroid preparations (halcinonide 0.1% and hydrocortisone l .0%) was assessed on ten healthy volunteers in a double blind study.
4. The effect of tachyphylaxis was observed with both the steroids within a week, still earlier with potent steroid.A four days rest was enough to bring back the skin to its pretreatment level.
P K Singh
Source of Support: None, Conflict of Interest: None