Effect of Angelica glauca essential oil on allergic airway changes induced by histamine and ovalbumin in experimental animals
Shilpa Sharma1, Vijaykumar P Rasal1, Paragouda A Patil2, Rajesh K Joshi3
1 Department of Pharmacology, KLE University, College of Pharmacy, Belgaum, Karnataka, India
2 Department of Pharmacology, USM KLE International Medical Programme, Belgaum, Karnataka, India
3 Department of Phytochemistry, Regional Medical Research Centre, Indian Council of Medical Research, Belgaum, Karnataka, India
Objective: Angelica glauca Edgew (Apiaceae) is used in traditional medicine for treatment of several diseases including bronchial asthma. The present investigation was aimed to evaluate broncho-relaxant activity of A. glauca essential oil in histamine and ovalbumin (OVA)-induced broncho constriction in experimental animals.
Materials and Methods: Airway was induced using histamine aerosol in guinea pigs (n = 24) and OVA aerosol in albino mice (n = 24). The number of inflammatory cells, namely, absolute eosinophils count in blood, total immunoglobulin E (IgE) in serum, eosinophils, and neutrophils in bronchoalveolar lavage fluid (BALF) and histopathological examination of lung tissues were investigated in A. glauca oil and dexamethasone-treated groups. A. glauca oil 200 μL/kg was given orally, and dexamethasone 2 mg/kg was given intraperitoneal. Both the treatments were repeated daily for 7 days. Results were analyzed by one-way ANOVA, and P ≤ 0.05 was considered statistically significant.
Results: Treatment with A. glauca essential oil significantly (P < 0.001) increased the time of preconvulsive dyspnea in histamine-induced guinea pigs. Oral treatment of A. glauca oil significantly (P < 0.001) decreased absolute blood eosinophil count (from 325 ± 3.69 to 200 ± 3.05 cells/mm3), serum level of IgE (from 6.10 ± 0.05 to 0.70 ± 0.08 IU/L), and the number of eosinophils (from 11.0% ±1.41% to 3.0% ±0.51%), neutrophils (from 13.0% ±1.12% to 5.0% ±1.39%) in BALF. Histopathological changes observed in lungs of untreated group were marked suppressed by treatment with A. glauca oil.
Conclusion: The essential oil of A. glauca has bronchorelaxation in both histamine and OVA-induced bronchoconstriction in animals. The traditional use of A. glauca against asthma could be attributed to its bronchodilator property as observed in the present study.
Rajesh K Joshi
Department of Phytochemistry, Regional Medical Research Centre, Indian Council of Medical Research, Belgaum, Karnataka
|How to cite this article:|
Sharma S, Rasal VP, Patil PA, Joshi RK. Effect of Angelica glauca essential oil on allergic airway changes induced by histamine and ovalbumin in experimental animals.Indian J Pharmacol 2017;49:55-59
|How to cite this URL:|
Sharma S, Rasal VP, Patil PA, Joshi RK. Effect of Angelica glauca essential oil on allergic airway changes induced by histamine and ovalbumin in experimental animals. Indian J Pharmacol [serial online] 2017 [cited 2020 Feb 26 ];49:55-59
Available from: http://www.ijp-online.com/article.asp?issn=0253-7613;year=2017;volume=49;issue=1;spage=55;epage=59;aulast=Sharma;type=0