Antidiabetic and antihyperlipidemic activities of a novel polyherbal formulation in high fat diet/streptozotocin induced diabetic rat model
N Subhasree1, Ananthkumar Kamella1, Ilango Kaliappan1, Aruna agrawal2, Govind Prasad Dubey3
1 Interdisciplinary Insitute of Indian System of Medicine, SRM University, Kattankulathur, Kancheepuram, Tamil Nadu, India
2 Department of Kriya Sharir, Faculty of Ayurveda, Institute of Medical Sciences, Varanasi, Uttar Pradesh, India
3 Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India
Objective: To investigate the antidiabetic and antihyperlipidemic activities of polyherbal formulation (PHF) containing hydroalcoholic extracts of four plants namely Salacia oblonga, Salacia roxbhurgii, Garcinia indica and Lagerstroemia parviflora in streptozotocin (STZ)-induced diabetic rats by administering oral doses (200 and 400 mg/kg body weight).
Materials and Methods: Animals were divided into diabetic and nondiabetic groups. Rats were fed with a high-fat diet (HFD) and induced with a single low dose of STZ (35 mg/kg) i.p. Diabetic rats were treated with formulation (200 and 400 mg/kg) and metformin 250 mg/kg. Blood glucose levels were measured using blood glucose test strips with ACCU CHEK glucometer. Lipid profile and gluconeogenic enzymes were determined in normal and STZ-induced diabetic rats after oral administration of the PHF for 28 days. Histopathological changes in diabetic rat organs (pancreas, liver, and kidney) were also observed after PHF treatment.
Results: Treatment of diabetic rats with PHF and metformin decreased plasma glucose and lipid profile levels. Blood glucose level showed significant reduction after 28 days of treatment with formulation at 200 and 400 mg/kg and in metformin. Formulation treated rats showed significant (P < 0.001) decrease in the activities of gluconeogenic enzymes. Histological examination of various organ tissues of normal control, diabetic control, and drug-treated rats revealed significant results. Treatment with PHF reverses the most blood and tissue changes toward the normal level.
Conclusion: These findings suggested the antihyperglycemic and antihyperlipidemic properties of the PHF and thus help in preventing future complications of diabetes.
Prof. Govind Prasad Dubey
Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh
|How to cite this article:|
Subhasree N, Kamella A, Kaliappan I, agrawal A, Dubey GP. Antidiabetic and antihyperlipidemic activities of a novel polyherbal formulation in high fat diet/streptozotocin induced diabetic rat model.Indian J Pharmacol 2015;47:509-513
|How to cite this URL:|
Subhasree N, Kamella A, Kaliappan I, agrawal A, Dubey GP. Antidiabetic and antihyperlipidemic activities of a novel polyherbal formulation in high fat diet/streptozotocin induced diabetic rat model. Indian J Pharmacol [serial online] 2015 [cited 2020 Feb 22 ];47:509-513
Available from: http://www.ijp-online.com/article.asp?issn=0253-7613;year=2015;volume=47;issue=5;spage=509;epage=513;aulast=Subhasree;type=0