The effect of chinese medicine pu-ren-dan on pancreatic angiogenesis in high fat diet/streptozotocin-induced diabetic rats
Binan Lu, Yongfei Bai, Ziliang Du, Shu Chen, D Deligema, Zongran Pang
Institute of Chinese Minority Traditional Medicine, Minzu University of China, Beijing 100081, China
Objectives: The islet vascular system is critical for β-cell function. This study investigated the antidiabetic effect of the Chinese Pu-Ren-Dan (PRD) recipe by regulating the pancreatic angiogenic factors in T2DM rats.
Materials Methods: High fat diet/streptozotocin-induced obese type-2 diabetes mellitus rats were developed and treated with PRD for 4 weeks. Then glucolipid metabolism, insulin secretion, pancreatic blood flow, ultrastructure of islet β-cell, histological changes of islet and protein expressions of pancreatic angiogenic factors were investigated.
Results: PRD-reduced T2DM rats«SQ» body weight and blood glucose level resisted the lipid metabolism disturbance, and ameliorated the insulin resistance and β-cell function. In addition, the histological and morphological studies proved that PRD could maintain the normal distribution of endocrine cell in islet and normal ultrastructure of β cell. An increased pancreatic blood flow was observed after the PRD treatment. In the investigation of pancreatic angiogenic factors, PRD inhibited the decreased expression of VEGF and Ang-1, and reversed the reduction of VEGFR2 and Tie2 phosphorylation in T2DM rats; the Ang-2 and TGFβ expression were up-regulated by PRD while PKC was activated; endostatin and angiostatin were down-regulated by PRD.
Conclusions: The results suggest that increasing VEGF expression, regulating VEGF/VEGFR2 signaling, stimulating Ang-1/Tie-2 signaling pathway, and inhibiting PKC-TGFβ signaling and antiangiogenic factors might be the underlying mechanism of PRD«SQ»s antidiabetic effect.
Institute of Chinese Minority Traditional Medicine, Minzu University of China, Beijing 100081
|How to cite this article:|
Lu B, Bai Y, Du Z, Chen S, Deligema D, Pang Z. The effect of chinese medicine pu-ren-dan on pancreatic angiogenesis in high fat diet/streptozotocin-induced diabetic rats.Indian J Pharmacol 2013;45:556-562
|How to cite this URL:|
Lu B, Bai Y, Du Z, Chen S, Deligema D, Pang Z. The effect of chinese medicine pu-ren-dan on pancreatic angiogenesis in high fat diet/streptozotocin-induced diabetic rats. Indian J Pharmacol [serial online] 2013 [cited 2020 Sep 26 ];45:556-562
Available from: http://www.ijp-online.com/article.asp?issn=0253-7613;year=2013;volume=45;issue=6;spage=556;epage=562;aulast=Lu;type=0