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|Year : 2007 | Volume
| Issue : 3 | Page : 145--150
Chlorpyrifos-induced oxidative stress and tissue damage in the liver, kidney, brain and fetus in pregnant rats: The protective role of the butanolic extract of Paronychia argentea L.
D Zama1, Z Meraihi2, S Tebibel1, W Benayssa3, F Benayache3, S Benayache3, AJ Vlietinck4,
1 Laboratory of Animal Physiology, Department of Animal Biology, Faculty of S. N. V. Constantine University, Algeria
2 Laboratory of Enzymology, Department of Biochemistry, Faculty of S.N.V, Constantine University, Algeria
3 Laboratory of Valorization of Natural Resources and Synthesis of Biological Active Substances, Department of Chemistry. Faculty of Sciences, Constantine University, Algeria
4 Laboratory of Pharmacognosie, Department of Pharmaceutical Sciences, University of Antwerp (UIA), Belgium
Laboratory of Animal Physiology, Department of Animal Biology, Faculty of S. N. V. Constantine University
Objective : Toxicity of pesticides is thought to be due to reactive oxygen species (ROS). Due to their antioxidant property, polyphenols in plant extracts may afford protection from pesticide toxicity. In the present study, we evaluated the protective effect of a butanolic extract of Paronychia argentea L. against toxicity caused by the organophosphorus pesticide, chloropyriphos ethyl (CE).
Materials and Methods : Pregnant albino Wistar rats were used. Pesticide and plant extract were administered daily by oral gavage from the 6 th to the 15 th day of gestation. Plasma and tissue malondialdehyde (MDA), blood reduced glutathione (GSH) and erythrocyte superoxide dismutase (SOD) activities were estimated. MDA levels were estimated in plasma and different organs (liver, kidney, brain, placenta and in the fetuses and their livers) as an indicator of lipid peroxydation (LPO).
Results : The data showed a significant increase in plasma and tissue LPO levels in animals treated with the pesticide while the effect was attenuated by the plant extract (CE-ex). Also, CE caused a significant decrease in antioxidant enzyme activity and this effect was partially reversed in groups treated with the plant extract. The pesticide induced embryotoxicity and resulted in resorption, fetal death and a reduced implant number.
Conclusion : It can be concluded that CE can lead to an increase in LPO production in adult and fetal tissues, while treatment with the plant extract leads to protection against CE toxicity. The decrease in LPO levels and the increase in GSH and SOD enzyme activities after treatment with the plant extract revealed its antioxidant property.
|How to cite this article:|
Zama D, Meraihi Z, Tebibel S, Benayssa W, Benayache F, Benayache S, Vlietinck A J. Chlorpyrifos-induced oxidative stress and tissue damage in the liver, kidney, brain and fetus in pregnant rats: The protective role of the butanolic extract of Paronychia argentea L. Indian J Pharmacol 2007;39:145-150
|How to cite this URL:|
Zama D, Meraihi Z, Tebibel S, Benayssa W, Benayache F, Benayache S, Vlietinck A J. Chlorpyrifos-induced oxidative stress and tissue damage in the liver, kidney, brain and fetus in pregnant rats: The protective role of the butanolic extract of Paronychia argentea L. Indian J Pharmacol [serial online] 2007 [cited 2020 Apr 2 ];39:145-150
Available from: http://www.ijp-online.com/text.asp?2007/39/3/145/33434
Pesticide chemicals can induce oxidative stress by generating free radicals and altering antioxidant levels of the free radical scavenging enzyme activity.  Chlorpyrifos (CE) is still widely used in Algeria as a pesticide for crops and farm animals. It induces toxicity through inhibition of acetyl cholinesterase (AChE) but also involves multiple mechanisms besides the inhibition of AChE.  Exposure to endosulfan and chlorpyrifos can differentially modify endogenous antioxidants like SOD, GPX and GSH, which can lead to the development of oxidative stress in some tissues.  Chlorpyrifos intoxication causes a significant decrease in the reduced glutathione (GSH), catalase (CAT) and glutathione- S -transferase (GST) activities. 
Plant extracts with antioxidant activity are traditionally used to strengthen the natural immune defences. Many studies have focused on the antioxidant effect of flavonoids,  resulting in their identification as potential antioxidants and anticancer agents. 
Several antioxidant dietary compound classes have been suggested to have health benefits. Evidence shows consumption of these products leads to a decrease in various pro-inflammatory and / or oxidative stress biomarkers. 
Paronychia argentea Lamb, which is widely distributed in East of Algeria, is used in folk medicine for the treatment of several disorders. Its aerial parts are used for the treatment of respiratory infections, abdominal pain and as an antistress agent. No published studies are available till date describing its pharmacological or antioxidant effects.
As this plant is already being widely used as an herbal tea in Algeria, it is important to elucidate the role of its extract in protection from the oxidative stress caused by CE.
Materials and Methods
1. Chemicals and plant extract
Chlorpyrifos ethyl (CE) [ O , O -diethyl- O -(3, 5, 6-trichloro-2-pyridyl) phosphorothioate], which is being widely used as an insecticide in Algeria, was used in this study. Fresh mature Paronychia argentea L. plants were collected from Mila, East of Algeria in May, 2003 and identified by Prof. Dr. Khalfallah N., S. N. V. Dept.; Constantine University, Algeria. Powdered, air-dried, aerial parts (1 kg) of Paronychia argentea were macerated thrice at room temperature with methanol-water (MeOH-H 2 O, (7:3 v/v) for 48 h. After filtration and concentration under reduced pressure, the residue was dissolved in water and re-extracted successively with chloroform (CHCl 3 ), ethyl acetate (EtOAc) and finally with n -butanol ( n -BuOH). The organic layers were dried over anhydrous Na 2 SO 4 (sodium sulfate) Then the solvents were removed under reduced pressure, leading to three extracts, CHCl 3 (6 g), EtOAc (8 g) and n -BuOH (36 g) extracts. In this study, we used the n -butanolic extract.
2. Experimental animals and treatments
Female albino Wistar rats, each weighing 175-195 g, were obtained from the Pasteur Institute, Algiers and randomized into eight groups of ten rats per group. A set of five rats was housed in a plastic cage in a temperature- and light-controlled room and the animals had free access to food and tap water. All animals were fed a commercial diet during the experiment. Daily doses of the pesticide and plant extract were administered by gavage from the 6 th to the 15 th day of gestation. The eight groups of rats fell into two categories. The first one served as a control and consisted of four groups out of which one was untreated and received only corn oil and the other three groups received 50, 100 or 200 mg/kg doses of the plant extract. The second category was also made up of four groups: one group was treated with CE (20 mg/kg) whereas the rats in the other three groups received the same dose of CE along with 50, 100 and 200 mg/kg doses of the plant extract. The plant extract was administered orally by gavage 15 min before oral administration of CE (20 mg/kg per day, once a day in corn oil) also by gavage. All dosing was started at the same time in the morning (0800 h) to avoid the effects of biological rhythm changes.
3. Methods of analysis
On day 19, animals were dissected under ether anesthesia and a blood sample was collected and used for the estimation of plasma malondialdhyde (MDA) as an indicator of lipid peroxidation (LPO) levels. An aliquot of heparinized blood was used for estimating GSH content. In addition, red blood cells were saved for estimation of SOD activity. After perfusion of the animal with ice-cold 0.9% NaCl through the jugular vein, the liver, kidney and brain were removed, weighed and homogenized in ice-cold potassium chloride (1.15% KCl) to give a 20% (w/v) tissue suspension. After centrifugation, the homogenate was used for the estimation of LPO levels.
Fetuses were removed, weighed and examined for any external congenital malformation. They were then assigned to two groups: the first group was homogenized in ice-cold 1.15% KCl to give 10% (w/v) tissue suspension. As mentioned earlier, the homogenate was separated by centrifugation and used for the determination of LPO levels in the fetus. The fetuses in the other group were dissected and their livers and placenta removed. These organs were treated and used as described before for both the estimation of placental and fetal liver LPO levels.
Lipid peroxidation assay
The formation of thiobarbituric acid-reactive substances (TBARs) in plasma and organs of adults (liver, kidney and brain) and of fetuses (placenta and liver) was monitored as an index of lipid peroxidation according to a previously described colorimetric method.  Briefly, 0.5 ml of plasma or 10% homogenate was taken in a 10 ml centrifuge tube to which 3 ml of 1% phosphoric acid and 1 ml of 0.6% aqueous thiobarbituric acid (TBA) were added. The mixture was heated for 45 min in a boiling water bath. After cooling, 4 ml of n -butanol was added and mixed vigorously. The butanol phase was separated by centrifugation and its absorbance was measured at 532 nm. Positive controls of MDA were also taken to determine the amounts of MDA formed in the organ / tissue homogenates, expressed as nanomoles of MDA per ml of plasma or per gram of wet tissue.
Glutathione level content
Determination of reduced glutathione level (GSH) in blood was carried out according to the previously described colorimetric method.  This method depends on the reaction of reduced glutathione with 5, 5-dithiobis, 2-(nitrobenzoic acid) that can be measured colorimetrically. The yellow color developed was measured at 412 nm against a blank reagent. GSH level was expressed as mg/dl blood.
Rats were fasted overnight and blood was withdrawn into EDTA. Samples were immediately centrifuged at 1500 g for 15 min. After separation of plasma, the packed erythrocytes were washed three times with a cold isotonic saline solution (0.9% NaCl) and centrifuged for 10 min at 1500 g . The washed red blood cells were lysed with ice-cold redistilled water. The suspension was centrifuged twice to obtain erythrocyte membranes and hemolysate. Hemoglobin was removed from the erythrocyte lysate by precipitating with ice-cold chloroform and ethanol. The remaining solution was shaken, centrifuged and the clear top layer was used for the determination of SOD enzyme activity.
Measurement of erythrocyte SOD activity was based on the SOD-mediated inhibition of the reaction of xanthine with xanthine oxidase to generate superoxide radicals, which react with 2-(4-iodophenyl)-3-(4-nitrophenol)-5-phenyltetrazolilum chloride to form a red formazan dye.  The SOD activity is expressed as IU/ml.
4. Statistical analysis
Statistical analysis was carried out using the SPSS for Windows Version 8.0 program. Data for each group of animals was subjected to analysis of variance (ANOVA). Values are given as mean ± standard deviation (SD). The statistical evaluation of the results was carried out using two-tailed, paired student's t- tests. Significance was set at P t- test was used to compare the difference between treated and control groups. Statistical significances of differences were calculated with one-way analyses of variance followed by Student-Newman-Keul's multiple-range test.
The effect of CE and plant extract on the fetus.
Rats treated with CE showed a significant decrease in fetal weights ( P P Fetus and implant number
A significant decrease ( P P P The effects of different treatments on plasma, organ and fetal LPO levels
Plasma LPO levels
The present study showed that CE caused a significant increase in TBAR levels. It was clearly demonstrated that plasma LPO levels increased significantly ( P P P Tissue LPO levels
Tissue MDA levels have been used as an indicator of LPO in vivo . As shown in [Figure 2]B, MDA levels in tissues of CE-treated rats were higher than those of control rats. These results support the concept that CE toxicity increases the susceptibility of tissues of the liver, kidney and brain to CE-mediated free radical damage. The data shown in [Figure 2]B demonstrates that CE treatment causes a significant increase ( P P Paronychia argentea L. protected cells and their subcellular structures from oxidative damage by decreasing MDA levels elevated due to exposure to pesticides or possibly other oxidative insults.
Placenta, fetal and fetal liver LPO levels
Data shown in [Figure 1] suggests that CE treatment affects fetal growth and results in decreased fetal weights. [Figure 3] shows a significant increase ( P P P The effect of CE and plant extract on GSH content and SOD enzyme activity
As shown in [Figure 4]A, CE treatment caused a significant decrease ( P SOD activity
The results in [Figure 4]B showed a significant decrease ( P P P. argentea L. protected the fetuses from this effect and caused a significant increase ( P P  using the same pesticide. In addition, another report describes a similar harmful impact on the development of preimplantation embryos in vitro and in vivo by another herbicide, paraquat. 
Literature reports claim that the central mechanism of pesticide action is via changes in the cellular oxidative status. Conclusive evidence demonstrates that supplementation with black tea extract protects against the free radical-mediated oxidative stress in hepatocytes of animals suffering from pesticide-induced live injury.  Endosulfan (13 mg/kg/day), an organochlorine insecticide, caused degeneration in mouse kidney due to oxidative stress.  Treatment with the insecticide, rotenone, increased xanthine oxidase enzyme activity and lipid peroxidation in liver tissue.  The pyrethroid insecticide, cypermethrin, significantly ( P  Isoflavone was found to confer notable protection against cypermethrin-induced oxidative stress in rabbit plasma, liver, brain and testes.  Levels of MDA, a major oxidation product of peroxidized polyunsaturated fatty acids, have been considered as an important indicator of lipid peroxidation.  In this study, we have shown that CE treatment increase MDA levels in plasma, liver, kidney and brain of the female rats as well as in the entire fetus and fetal liver and placenta. Plant extract treatment was found to significantly ( P  Thus, it appears that the orally administered butanolic extract of Paronychia argentea protects against CE-induced toxicity possibly through the inhibition of increased LPO in addition to inhibition of triglyceride accumulation, plasma membrane destruction and neutrophil infiltration in the liver tissue.
Glutathione is the cell's natural antioxidant, which destroys free radicals formed in cells. Significant dose-dependent depletion of GSH levels and perturbations in antioxidant enzyme levels further confirmed the potential of the insecticide, fenvalerate to induce oxidative stress in hepatic tissue.  Subchronic exposure to the insecticide, dimethoate (6 and 30 mg/kg) resulted in a decrease in glutathione levels in both liver and brain tissues of male Wistar rats.  It was concluded that the oxidative stress due to dimethoate may be ascribed to the induction of Cytochrome P450, inhibition of AChE and disturbance in the activities of GSH and GST enzymes causing lipid peroxidation and histological changes in the liver and the brain.  In this present study, we observed a similar decrease in GSH levels in the pregnant female rats treated with CE. Co-administration of the plant extract with CE was found to restore the GSH levels to those found in control rats.Besides the decrease in GSH and increase in MDA (LPO) levels, we found that CE-treated rats also showed a significant decrease in SOD activity. This decrease in SOD activity could be due to the increased production of ROS as evident from the increased LPO levels due to CE treatment. However, a few studies have indicated that superoxide radicals can also inhibit catalase (CAT) activity and the increased H 2 O 2 levels resulting from CAT inhibition could finally inhibit SOD activity. Thus, increased formation of MDA could be due to both increase in pesticide-induced ROS formation and SOD inhibition.  In contrast, plant extract treatment was found to significantly increase SOD enzyme activity in the erythrocytes of the pregnant rats, which could explain the decrease in LPO levels.  Increase in SOD activity should also accelerate the removal of the ROS. However, it is not clear how this plant extract increases SOD activity. There may be two explanations for this effect: plant extract may directly affect enzyme transcription or decrease superoxide production.
In conclusion, the present study suggests that the butanolic extract of Paronychia argentea L. can prevent or slow down the oxidative damage induced by CE in rats. The effects of CE on LPO and GSH levels and on SOD activity were reversed by treatment with plant extract. Further studies to identify the active compounds in the butanolic extract of Paronychia argentea L. and determine their structure and mechanism of action are in progress. In addition, further work is required to clarify how this plant extract increases antioxidant enzyme activity (directly by gene transcription or indirectly through superoxide production).
The authors express their sincere thanks to Professor Dr. Kalfallah N. (Constantine University, Algeria) for her help for the identification of the plant. They are also very grateful to Dr. Baaziz N. (Constantine University) and to Dr. S. KHENNOUF (Setif University) for their help in correction and critical review of the manuscript and to Dr. K. SEMRA for final correction of the translation. This study was carried out with financial support from project F2501/05/2005.
|1||Sharma Y, Bashir S, Irshad M, Gupta SD, Dogra TD. Effects of acute dimethoate administration on antioxidant status of liver and brain of experimental rats. Toxicology 2005;206:49-57.|
|2||Slotkin TA, Olivier CA, Seidler FJ. Critical periods for the role of oxidative stress in the developmental neurotoxicity of chlorpyrifos and terbutaline, alone or in combination. Brain Res Dev Brain Res 2005;157:172-80.|
|3||Bebe FN, Panemangalore M. Exposure to low doses of endosulfan and chlorpyrifos modifies endogenous antioxidants in tissues of rats. J Environ Sci Health B 2003;38:349-63.|
|4||Goel A, Danni V, Dhawan DK. Protective effects of zinc on lipid peroxidation, antioxidant enzymes and hepatic histoarchitecture in chlorpyrifos-induced toxicity. Chem Biol Interact 2005;156:131-40.|
|5||Gao Z, Xu H, Chen X, Chen K. Antioxidant status and mineral contents in tissues of rutin and baicalin fed rats. Life Sci 2003;73:1599-607.|
|6||Lee JY, Hwang WI, Lim ST. Antioxidant and anticancer activities of organic extracts from Platycodon grandiflorum A . De Condole roots. J Ethnopharmacol 2004;93:409-15.|
|7||Vouldoukis I, Lacan D, Kamate C, Coste P, Calenda A, Mazier D, et al . Antioxidant and anti-inflammatory properties of a Cucumis melo LC. Extract rich in superoxide dismutase activity. J Ethnopharmacol 2004;94:67-75.|
|8||Selvendiran K, Singh JP, Krishnan KB, Sakthisekaran D. Cytoprotective effect of piperine against benzo[a]pyrene induced lung cancer with reference to lipid peroxidation and antioxidant system in Swiss albino mice. Fitoterapia 2003;74:109-15.|
|9||Mihara M, Uchiyama M. Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal Biochem 1978;86:271-8.|
|10||Beutler E, Duran O, Kelly BM. Improved method for determination of blood glutathione. J Lab Clin Med 1963;61:882-8.|
|11||Woolliams JA, Wiener G, Anderson PH, McMurray CH. Variation in the activities of glutathione peroxidase and superoxide dismutase and in the concentration of copper in the blood of various breed crosses cheep. Res Vet Sci 1983;34:253-6.|
|12||Hunter DL, Lassiter TL, Padilla S, Gestational exposure to chlorpyrifos: Comparative distribution of trichloropyridinol in the fetus and dam. Toxicol Appl Pharmacol 1999;158:16-23.|
|13||Hausburg MA, Dekrey GK, Salmen JJ, Palic MR, Gardiner CS. Effects of paraquat on development of preimplantation embryos in vivo and in vitro . Reprod Toxicol 2005;20:239-46.|
|14||Kalender S, Kalender Y, Ogutcu A, Uzunhisarcikli M, Durak D, Aηikgoz F. Endosulfan-induced cardiotoxicity and free radical metabolism in rats:the protective effect of vitamin E. Toxicology 2004;202:227-35.|
|15||Caglar Y, Kaya M, Belge E, Mete UO. Ultrastructural evaluation of the effect of endosulfan on mice kidney. Histol Histopathol 2003;18:703-8.|
|16||Zama D, Meraihi Z, Boubekri N, Amrani A, Tebibel S, Baali N. Assessment of the changes in some diagnostic enzymes and other parameters in wistar albino rats treated with pesticides during gestation. Sciences and Technologie de l'Universitι Mentouri Constantine N°23, Juin 2005.|
|17||Terzi A, Iraz M, Sahin S, Ilhan A, Idiz N, Fadillioglu E. Protective effects of erdosteine on rotenone-induced oxidant injury in liver tissue. Toxicol Ind Health 2004;20:141-7.|
|18||el-Demerdash FM, Yousef MI, Al-Salhen KS. Protective effects of isoflavone on some biochemical parameters affected by cypermethrin in male rabbits. J Environ Sci Health B 2003;38:365-78.|
|19||Khan SM. Protective effect of black tea extract on the levels of lipid peroxidation and antioxidant enzymes in liver of mice with pesticide-induced liver injury. Cell Biochem Funct 2005;24:327-32.|
|20||Hassoun E, Stohs SJ. TCDD, Endrin and Lindane induced oxidative stress in fetal and placental tissues of C57BL /6J and DBA / 2J Mice. Comp Biochem Physiol C Pharmacol Toxicol Endocrinol 1996;115:11-8.|
|21||Prasanthi K, Muralidhara, Rajini PS. Fenvalerate-induced oxidative damage in rat tissues and its attenuation by dietary sesame oil. Food Chem Toxicol 2005;43:299-306.|
|22||Sharma Y, Bashir S, Irshad M, Nag TC, Dogra TD. Dimethoate-induced effects on antioxidant status of liver and brain of rats following sub chronic exposure. Toxicology 2005;215:173-81.|
|23||Gultekin F, Delibas N, Yasar S, Killinc I. In vivo changes in antioxidant systems and protective role of melatonin and a combination of vitamin C and vitamin E on oxidative damage in erythrocytes induced by chlorpyrifos-ethyl in rats. Arch Toxicol 2001;75:88-96.|
|24||Dasgupta T, Banerjee S, Yadava PK, Rao AR. Chemopreventive potential of Azadirachta indica ( Neem) leaf extract in murine carcinogenesis model systems. J Ethnopharmacol 2004;92:23-36.|