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|Year : 1999 | Volume
| Issue : 2 | Page : 146--149
Inhibition of nitric oxide synthase by Nw-nitro-l-arginine overcomes delayed intestinal transit in streptozotocin-induced diabetic mice
BM Patil1, KM Thomas2
Objectives: To characterize the changes in intestinal transit induced by diabetes and the implication of nitric oxide in such changes.
Methods: Diabetes was induced in mice by single injection of streptozotocin (STZ 160 mg/kg, ip). One, four and six weeks later, intestinal transit was measured after pretreatment with saline or drug, using charcoal meal method.
Results: Intestinal transit was significantly reduced in 1, 4 and 6 weeks STZ diabetic mice as compared with normal mice. Treatment with N(-nitro-L-arginine (10,20and 25 mg/kg, ip ) dose dependently increased the intestinal transit in STZ-diabetic mice. Increased intestinal transit effect of N(-nitro-L-arginine (20 mg/kg) was dose dependently reversed by L-arginine (300, 600 and 900 mg/kg ) but not by D-arginine (600 mg/kg ).
Conclusion: In the early stages of STZ diabetes, there may be an enhanced production of nitric oxide, which could account for reduced intestinal transit in diabetes.
B M Patil
|How to cite this article:|
Patil B M, Thomas K M. Inhibition of nitric oxide synthase by Nw-nitro-l-arginine overcomes delayed intestinal transit in streptozotocin-induced diabetic mice.Indian J Pharmacol 1999;31:146-149
|How to cite this URL:|
Patil B M, Thomas K M. Inhibition of nitric oxide synthase by Nw-nitro-l-arginine overcomes delayed intestinal transit in streptozotocin-induced diabetic mice. Indian J Pharmacol [serial online] 1999 [cited 2020 Sep 21 ];31:146-149
Available from: http://www.ijp-online.com/article.asp?issn=0253-7613;year=1999;volume=31;issue=2;spage=146;epage=149;aulast=Patil;type=0