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 EXPERIMENTAL RESEARCH ARTICLE
Year : 2020  |  Volume : 52  |  Issue : 2  |  Page : 108-116

Carthamus tinctorius L. Extract ameliorates cerebral ischemia-reperfusion injury in rats by regulating matrix metalloproteinases and apoptosis


1 School of Animal Engineering, Xuzhou Vocational College of Bioengineering, Xuzhou, China
2 School of Traditional Chinese Pharmacy, China Pharmaceutical University; Jumpcan Pharmaceutical Co., Ltd, Jiangsu Taixing, Nanjing, China
3 School of Traditional Chinese Pharmacy, China Pharmaceutical University; College of Pharmacy, Jiangsu University, Zhenjiang, China
4 Department of Cardiology, Nanjing Drum Tower Hospital, Clinical College of Nanjing Medical University, Nanjing, China
5 School of Traditional Chinese Pharmacy, China Pharmaceutical University, Taixing, China
6 College of Pharmacy, Xuzhou Medical University, Xuzhou, Jiangsu, China
7 College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China
8 School of Traditional Chinese Pharmacy, China Pharmaceutical University; Jumpcan Pharmaceutical Co., Ltd, Jiangsu Taixing; College of Pharmacy, Xuzhou Medical University, Xuzhou, Jiangsu, China

Correspondence Address:
Jun-Fei Gu
Nanjing University of Chinese Medicine, Nanjing 210023
China
Liang Feng
School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China. Jumpcan Pharmaceutical Co., Ltd, Jiangsu Taixing 225400; College of Pharmacy, Xuzhou Medical University, Xuzhou, Jiangsu 221002
China
Yi-Qiang An
College of Pharmacy, Xuzhou Medical University, Xuzhou, Jiangsu 221002
China
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/ijp.IJP_400_18

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We investigate the protective effect of Carthamus tinctorius L. (CTL, also known as Honghua in China or Safflower) on cerebral ischemia-reperfusion and explored the possible mechanisms on regulating apoptosis and matrix metalloproteinases (MMPs). High-performance liquid chromatography method with diode array detection analysis was established to analyze the components of CTL. Middle cerebral artery occlusion rats model was established to evaluate Neurological Function Score and hematoxylin-eosin staining, as well as triphenyltetrazolium was used to examine the infarction area ratio. Transferase-mediated dUTP nick-end labeling was performed for the apoptosis. Apoptosis-related factors, including B-cell lymphoma-2 (Bcl-2), Bax and Caspase3, and MMPs-related MMP2, MMP9, tissue inhibitor of metalloproteinases 1 (TIMP1) in ischemic brain, were assayed by Western blot, reverse transcription polymerase chain reaction, and immunohistochemistry. The data showed that CTL (2, 4 g crude drug/kg/d) treatment could significantly reduce the ischemic damage in brain tissue and improve a significant neurological function score. In addition, CTL could also attenuate apoptosis degree of brain tissues and regulate Bcl-2, Bax, and Caspase 3 and also have a significant decrease on MMP-9 expression, followed by a significant increase of TIMP1 protein expression. These findings indicated that regulation of CTL on apoptosis and MMPs contributed to its protective effect on ischemia/reperfusion injury.






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