| EXPERIMENTAL RESEARCH ARTICLE
|Year : 2020 | Volume
| Issue : 2 | Page : 102-107
SB365 induces apoptosis and suppresses proliferation of glioblastoma cells
Joo Han Lim, Kyung Hee Jung, Mi-Soon Kim, Jee Hyeon You, In-Suh Park, Soon-Sun Hong
Department of Medicine, College of Medicine, Inha University, Incheon, Republic of Korea
CONTEXT: Glioblastoma is a malignant brain tumor with limited treatment modalities due to its nature. SB365, Pulsatilla saponin D, is known to induce apoptosis and inhibit the growth of many cancer cells.
AIM: We elucidated the anticancer effects of SB365 in glioblastoma cells.
METHODS: We examined the antiproliferative activity of SB365 in human glioblastoma cell lines. Apoptosis was evaluated using the Hoechst assay, TUNEL assay, DAPI nuclear staining, and Western blotting analysis. To test the antimetastatic capacity of SB365, cell migration assay was conducted, and hypoxia-inducible factor-1 alpha (HIF-1α) expression and vascular endothelial growth factor (VEGF) level were determined under hypoxic conditions.
STATICAL ANALYSIS: Significance of the results was confirmed by a one-way analysis of variance analysis.
RESULTS: SB365 treatment suppressed the growth of glioblastoma cells and resulted in apoptotic morphological features such as nuclear condensation and fragmentation, enhancing the expression of cleaved poly (ADP-ribose) polymerase and caspase-3. It also significantly delayed cell migration and decreased the HIF-1α expression and VEGF secretion.
CONCLUSION: Our findings thus demonstrate that SB365 induced apoptosis and delayed the growth and migration of human glioblastoma cells. It is considered that SB365 would be a promising therapeutic option for glioblastoma.
Department of Pathology, College of Medicine, Inha University, 366 Seohae-Daero, Jung-Gu, Incheon 22332
Republic of Korea
Department of Biomedical Sciences, College of Medicine, Inha University, 366 Seohae Daero, Jung Gu, Incheon 22332
Republic of Korea
Source of Support: None, Conflict of Interest: None
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