| RESEARCH ARTICLE
|Year : 2018 | Volume
| Issue : 6 | Page : 336-343
Sulfated polysaccharides of seagrass Halophila ovalis suppresses tumor necrosis factor-α-induced chemokine interleukin-8 secretion in HT-29 cell line
Neelakandan Yuvaraj1, Venkatesan Arul2
1 Department of Biotechnology, Achariya Arts and Science College, Puducherry, India
2 Department of Biotechnology, School of Life Sciences, Pondicherry University, Puducherry, India
OBJECTIVES: The present study aims to investigate the anti-oxidant and anti-inflammatory properties of seagrass Halophila ovalis sulfated polysaccharide on HT-29 cell line.
SUBJECTS AND METHODS: Monosaccharides composition was identified using liquid chromatography-mass spectrometry (LC-MS) and the functional groups were analyzed using Fourier transform-infrared (FT-IR) spectroscopy. The antioxidant and anti-inflammatory potential of crude extract and purified fractions was investigated in vitro.
RESULTS: FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS. Ho-SP and its fractions showed radical scavenging activity in hydroxyl, 2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid, and ferric reducing antioxidant power assay in a dose-dependent manner. Noticeable anti-inflammatory activity of purified fraction Ho FrIV (IC50= 43.85 μg/ml) was observed in a noncytotoxic range of concentrations and inhibited the tumor necrosis factor-α (TNF-α)-induced interleukin-8 (IL-8) secretion (0.27 ng/ml) in HT-29 cell line.
CONCLUSION: Overall, the results presented in this study suggest that purified fraction Ho FrIV of Ho-SP could suppress the TNF-α-induced secretion of IL-8 in HT-29 and thus could be used as a promising antioxidant and anti-inflammatory candidate with potential benefits.
Dr. Neelakandan Yuvaraj
No. 6, Main Road, Aranganur, Puducherry - 607 402
Source of Support: None, Conflict of Interest: None
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