|Year : 2018 | Volume
| Issue : 3 | Page : 123-129
Gas chromatography coupled to mass spectrometry characterization, anti-inflammatory effect, wound-healing potential, and hair growth-promoting activity of Algerian Carthamus caeruleus L (Asteraceae)
Mohammed Mahdi Dahmani1, Razika Laoufi2, Okba Selama3, Karim Arab1
1 Department of Biology, Faculty of Science, Valorization and Conservation Laboratory of Biological Resources, University of Boumerdes, Algeria
2 Department of Biology, Faculty of Science, Laboratory Technologies Sweet, Valorisation, Physical Chemistry of Biological Materials and Biodiversity, University of Boumerdes, Algeria
3 Department of Biology, Faculty of Biological Sciences, Laboratory of Cellular and Molecular Biology, USTHB, Ezzouar, Algiers, Algeria; Department of Biology, School of Life Sciences, University of Warwick, Coventry, England, UK
|Date of Submission||03-Sep-2017|
|Date of Acceptance||24-Jun-2018|
|Date of Web Publication||16-Aug-2018|
Mohammed Mahdi Dahmani
Department of Biology, Faculty of Science, Valorization and Conservation Laboratory of Biological Resources, University of Boumerdes, Algiers
Source of Support: None, Conflict of Interest: None
OBJECTIVES: The roots of Carthamus caeruleus have been used by the population of Northern Algeria to treat several pathological conditions, including wound healing and hair growth. The present study was conducted to evaluate the anti-inflammatory activity, wound-healing potential, and hair growth-promoting activity attributed to C. caeruleus root.
MATERIALS AND METHODS: In this study, we have investigated the anti-inflammatory effect using carrageenan-induced paw edema test, evaluated the wound-healing potential by linear incision wound model, and evaluated hair growth activity using in vivo hair growth-promoting test attributed to C. caeruleus root. Preliminary phytochemical screening and gas chromatography coupled to mass spectrometry (GC/MS) characterization were also performed.
RESULTS: It was found that the methanolic extract of C. caeruleus was characterized by the presence of tannins, flavonoids, anthocyanins, leucoanthocyanins, sennosides, free quinones, saponins, glycosides, mucilage, and coumarins. The GC/MS analysis could identify 22 compounds and showed that the major chemical constituents were palmitic acid (12.88%), mono(2-ethylhexyl) phthalate (12.75%), and 5-(hydroxymethyl)-2-furancarboxaldehyde, (9.19%). The phytoextract strongly inhibited (P < 0.001) paw edema formation in mice. The roots of C. caeruleus also showed a significant (P < 0.05) wound-healing and hair growth-promoting effects.
CONCLUSION: The results indicate the richness of the roots of the Algerian C. caeruleus in biomolecules. These molecules exhibit an excellent reducing inflammation activity, a wound-healing property, and an interesting hair-promoting growth activity. All in all, the findings promote the usage of the Algerian C. caeruleus as an effective and a safe potential skincare alternative remedy.
Keywords: Biomolecules, Carthamus caeruleus, gas chromatography coupled to mass spectrometry, skincare
|How to cite this article:|
Dahmani MM, Laoufi R, Selama O, Arab K. Gas chromatography coupled to mass spectrometry characterization, anti-inflammatory effect, wound-healing potential, and hair growth-promoting activity of Algerian Carthamus caeruleus L (Asteraceae). Indian J Pharmacol 2018;50:123-9
|How to cite this URL:|
Dahmani MM, Laoufi R, Selama O, Arab K. Gas chromatography coupled to mass spectrometry characterization, anti-inflammatory effect, wound-healing potential, and hair growth-promoting activity of Algerian Carthamus caeruleus L (Asteraceae). Indian J Pharmacol [serial online] 2018 [cited 2020 Jul 8];50:123-9. Available from: http://www.ijp-online.com/text.asp?2018/50/3/123/239058
| » Introduction|| |
Fighting diseases has been an important aspect of human beings interactions with natural environment. In this fight, the scientific interest has been diverted toward the natural compounds as they are biocompatible, safe, and cost-effective as well. Plants have been forever a major ally in the healing process. Treatment with medicinal plants is considered very safe as there is no or minimal side effect. In addition, use of herbal treatments is independent of any age groups and the sexes. On the other hand, medicinal plants are common and important sources of therapeutic biomolecules comparing to marine organisms, microorganisms, and fungi. They contain a wide diversity of chemical entities for the development of drugs against various pharmacological targets. Accurate identification and determination of the chemical components from herbal plants will aid in predicting their biological actions.
Inflammation is a complex biological response of vascular tissues to invasion by an infectious agent, antigen challenge, physical, chemical, or traumatic damage. A striking feature of allopathic medications is the debilitating and lethal consequences of adverse drug reactions (ADRs), which rank as one of the top 10 causes of death and illness in the developed world, claiming 100,000–218,000 lives in the United States annually. Currently used anti-inflammatory drugs are associated with some severe side effects. Common adverse effects such as gastric lesions caused by nonsteroidal anti-inflammatory drugs (NSAIDs) and tolerance and dependence induced by opiates have been registered; as a result, the use of these drugs as anti-inflammatory and analgesic agents have not been successful in all cases. Thus, the development and the investigation of potent anti-inflammatory and analgesic drugs with fewer or lacking side effects are necessary; these could substitute NSAIDs and opiates. Several studies have reported an anti-inflammatory effect and an antitumor activity of Carthamus sp.
Wound is a rupture in the epithelial integrity of the skin which resulted from violence or trauma and may be followed by disruption of the structure and function of underlying normal tissue. Each year, millions of people experience burns, suffer from chronic wounds, or have acute wounds that become complicated by infection, dehiscence, or problematic scarring. Therefore, more efforts toward the development of new lead compounds for effective and evidence-based wound-healing strategies are needed. Recent investigations have shown that plant-derived secondary compounds can serve as new lead compounds for the improvement of wound healing. Polyphenols are used nonspecifically against wound infection and stimulate keratinocyte proliferation, and oligo-polysaccharides induce proliferation of keratinocytes.
The term androgenetic alopecia frequently used to express the patterned loss of scalp hair in genetically vulnerable men and women. To address this problem, the markets of cosmetics and pharmaceuticals for the hair regrowth and protection from hair loss have been markedly growing for a few decades. In dermatology, Carthamus tinctorius has many beneficial effects against skin problems such as erythematic, psoriasis, and other dermatitis.
The Carthamus caeruleus species growing in the north of Algeria is commonly used in traditional medicine by healers and local population, because of their therapeutic benefits to treat skin problems and burns. The aims of this study were to evaluate the anti-inflammatory, wound-healing, and hair growth-promoting activities of the roots of C. caeruleus variety.
| » Materials and Methods|| |
Harvesting and preparing the plant powder
The harvesting of the plant was conducted in March and April 2014, under hot and dry weather after sunrise, to avoid wetting of the plant. Samples of roots, free of insects and molluscs, were retained. Location of sampling region was in Draâ El Mizan (a municipality in the Wilaya of Tizi Ouzou in Algeria, located 42 km southwest of Tizi Ouzou and 110 km southeast of Algiers).
Preparation of extract
The roots of C. caeruleus are dried in open air and at obscure for a week at room temperature. The plant material is crushed into a clean mortar and then finely ground using an electric grinder. The obtained plant powder (20 g) was macerated in 100 ml of methanol (95%) for 3 days and then proceeded to a purification of the extract from residues. After filtration, the alcoholic layer was evaporated under reduced pressure by a rotary evaporator at 70°C. The obtained filtrate was stored at 4°C until use.
A set of colorimetric methods were carried out on either the plant powder or the infusion, and this allows the detection of secondary metabolites. We searched mainly anthocyanins, leucoanthocyanins, total tannins, irridoids, gallic tannins, catechin tannins, alkaloids, flavonoids, saponosids, senosids, quinones, coumarins, and mucilages. Methods in this study were conducted as described by Yadav and Agarwala.
Gas chromatography coupled to mass spectrometry
The analysis of the methanolic extract was performed on a Hewlett–Packard gas chromatography coupled to mass spectrometry (GC/MS) system (GC 6890, 5973 MSD). A capillary column HP-5 MS (30 m, 0.25 mm, and film thickness 0.25 lm) is directly coupled to the mass spectrometer. The carrier gas used was helium (1 ml/min). The temperature program used was 3 min isothermal at 60°C, then 270°C at a rate of 5°C/min, and then held for 10 min isothermal. The injector temperature was 250°C. The ionization of sample components was performed by electron ionization mode (70 eV).
The compounds are detected in order of elution and identified by their mass spectrum and retention time. Reading the chromatographic profiles is provided by a computer system managing a mass spectra library: NIST002 (National Institute of Standards and Technology).
Male NMRI mice (20–25 g) and Wistar rats (130–160 g) provided by Pasteur Institute of Algeria were left for 3 days for adaptation period into laboratory conditions and maintained on standard pellet diet and water. For the evaluation of pharmacological activities, the food was withdrawn on the day before the experiment, but free access to water was allowed. Six animals were used in each group. All experiments complied with the Algerian Legislation (Law Number 12-235/2012) inherent to the protection of animals designed to experimental and other scientific purposes as well as with the guidelines of the Algerian Association of Experimental Animal Sciences (AASEA) and were specifically approved by the latter (AASEA authorization number 45/DGLPAG/DVA/SDA/14).
In this test, the used mice were divided into three groups (six mice per group). The different groups were treated intraperitoneal with methanolic extract of C. caeruleus (2.5 ml/kg), diclofenac (2.5 ml/kg), and vehicle control (0.9% NaCl). The administration of extract and drugs was 1 h before injection of 0.025 ml of 1% freshly prepared suspension of carrageenan (in 0.9% NaCl) in the right hind paw subplantar of each mouse. The paw volume was measured 4 h later, the animals were killed by cervical dislocation, and the right and left paw of each animal were removed. The left paw was considered as a control. Circular sections were taken with a cork borer (diameter of 7 mm) and weighed. The edematous response was measured as the weight difference between the two plugs. The anti-inflammatory activity was expressed as a percentage of the edema reduction in treated mice compared to the control group.
The anti-inflammatory effect of methanolic extract of C. caeruleus was calculated by the following equation: Anti-inflammatory activity (%) = (1 − D/C) × 100, where D represents the percentage difference in paw volume after the administration of drugs to the mouse and C represents the percentage difference of volume in the control groups.
Linear incision wound model
Animals were anesthetized with 0.05 mL xylazine (2% Alfazine ®) and 0.15 mL ketamine (10% Ketasol ®) and the back hair of the rats were shaved and cleaned with 70% alcohol. Two 1-cm length linear-paravertebral incisions were created with a sterile blade at a distance of 0.5 cm from the dorsal midline on each side. The test ointments, the reference drug (Madecassol®), and the ointment base were topically applied on the dorsal wounds in each group of animals once daily throughout 9 days.
Percent wound contraction
The wound contraction of individual animal from control and treatment groups was measured in each and healing rate was expressed as percentage contraction:
Percent wound contraction = healed area/total area × 100
In vivo hair growth-promoting test
The animals were divided into two randomized groups (n = 6) to study the hair growth-promoting activity of the C. caeruleus root. All animals were shaved by clipping to observe the anagen phase hair follicle development. Daily treatment was applied for 9 days.
Pharmacological data were presented as mean ± standard error (SD). Student's t-test was used to assess the differences between groups using P < 0.05 as the level of statistical difference.
| » Results|| |
The results show that C caeruleus is rich in tannins, flavonoids, anthocyanins, leucoanthocyanins, sennosides, free quinones, saponins, glycosides, mucilage, and coumarins [Table 1].
Chromatographic analysis of Carthamus caeruleus roots extract
[Figure 1] represents the GC-MS chromatogram of C. caeruleus roots extract, which showed a set of peaks indicating the presence of the phytochemical compounds.
|Figure 1: GC/MS chromatogram of Carthamus caeruleus roots methanolic extract|
Click here to view
The active principles of the roots with their retention time and peak area in percentage are presented in [Table 2].
|Table 2: Gas chromatography coupled to mass spectroscopy results of the bioactive compounds identified in the methanolic extract of Carthamus caeruleus|
Click here to view
Percentage increase in paw volume was significantly reduced in treated mice after 4 h pretreatment with C. caeruleus extract compared to negative controls. As expected for Diclofenac ® sodium with its anti-inflammatory effect, an important diminution in percentage increase in paw volume induced by carrageenan was observed. The anti-inflammatory activity of methanolic extract of C. caeruleus was very important following postinjection showing a greater anti-inflammatory activity similar to the positive controls (Diclofenac ®) [Figure 2].
|Figure 2: Percentage inhibition of edema by methanol extract of Carthamus caeruleus determined with Diclofenac sodium as reference (values are mean ± standard deviation, ***P < 0.001, compared to control, n = 6)|
Click here to view
Wound contraction was tested to determine the wound rate reduction during healing. The fast rate of wound closer indicates the better efficacy. The progressive reduction in wound area of different groups of treated animals over 9 days for the negative control, the ointment of C. caeruleus, and Madicasol ® is presented in [Figure 3]. The fastest and complete (98.33% ± 4%) wound contraction healing was observed in animals treated with the ointment of C. caeruleus, compared to Madicasol ® (86.66% ± 15.05%) and the negative control (81.66% ± 15.05%).
|Figure 3: Effect of Carthamus caeruleus ointments on wound contraction in incision wound model (values are mean ± standard deviation, *P < 0.05, compared to control, n = 6)|
Click here to view
Effect of Carthamus caeruleus ointment on anagen induction
Wistar rats were used to investigate the effect of C. caeruleus ointment on anagen phase induction. The shaved skin of telogen phase rats is pink and whiteness with the initiation of anagen. The day after the animals were shaved using a clipper, the dorsal skin of these rats was treated with C. caeruleus ointment or negative control daily for 9 days. Visual scoring of the hair growth-promoting effect of Carthamus caeruleus ointments by using the scoring guideline shown in [Table 3]. C. caeruleus ointment-treated groups exhibited a significant air growth (4.83 ± 0.98) compared to negative control (0.67 ± 0.81) [Figure 4].
|Figure 4: Effect of Carthamus caeruleus ointments on hair growth in Wistar rats. (Values are mean ± standard deviation, ***P < 0.001, compared to control, n = 6)|
Click here to view
| » Discussion|| |
The phytochemical screening of C. caeruleus showed an important wealth in tannins, flavonoids, anthocyanins, leucoanthocyanins, sennosides, saponins, glycosides, mucilage, and coumarins. The existing literature reports that these chemical components present several pharmacological activities.
GC/MS analysis plays a key role in the analysis of components of plant origin. Generally, the plant materials are highly complexes, which make GC/MS well suited for their analysis because of its high sensitivity and selectivity. The main compounds are n-hexadecanoic acid (palmitic acid), mono (2-ethylhexyl) phthalate (MEHP), 5-(hydroxymethyl)-2-furancarboxaldehyde (5-HMF), and 8-methylene-3-oxatricyclo[126.96.36.199(2,4)]nonane. To the best of our knowledge, this is the first time the roots in this species were analyzed by GC/MS. The GC/MS analysis of C. caeruleus showed the presence of MEHP the biomolecule with multiple biological activities such as antifungal, antiretroviral, antitumor, antidiabetic, antioxidant, anti-inflammatory, and antimicrobial. A number of other studies have found additional therapeutic properties including anti-inflammatory.
Methanolic extracts of C. caeruleus roots produced a significant inhibition of carrageenan-induced paw edema. The inhibition was more important than that of the standard drug Diclofenac ® sodium. There are several mediators or multiprocesses underlining the pathogenesis of inflammatory. Inhibition of any of these mediators may bring down the inflammatory process. In a preliminary phytochemical screening of our methanolic extract of C. caeruleus roots, we have found several compounds among flavonoids which are known to target prostaglandins, which are involved in the late phase of acute inflammation and pain perception. Another major biomolecule of C. caeruleus root, palmitic acid, might function as an anti-inflammatory agent as it had shown a significant inhibitory activity in the enzyme kinetic study of phospholipase A. Pharmacological studies on the components showed that 5-HMF had good biological activities, such as anti-inflammatory activity and bacteriostatic action. Moreover, the caryophyllene oxide shows analgesic and anti-inflammatory activities.
Wound healing is an interactive process involving mediators, cells, and extracellular matrix components and has three successive stages; inflammation, tissue formation, and tissue remodeling that overlap in time. Traditional wound-healing remedies are expected to contribute the wound-healing process by acting in one or more of these stages. Medicinal plant-based ointments used in folk medicine are reported to have beneficial effects in wound care and healing. The wound-healing activity of C. caeruleus ointments from the underground part of this medicinal plant was evaluated comparatively.
According to the experimental results, C. caeruleus possessed better wound-healing activity compared to Madecasol ® and the control groups. Several factors could be involved in the enhancement of the wound-healing process. The presence of the antioxidant, anti-inflammatory, and antimicrobials properties may be among the factors that contributed to the potential wound-healing effect of C. caeruleus root. Moreover, interesting in vitro pharmacological activities of C. caeruleus have been reported such as antimicrobial and antioxidant activities.
Pharmacological tests showed that the lipid concentration from the medicinal plant had a healing activity. Furthermore, the main compound of the roots of C. caeruleus is palmitic acid; this molecule induces the wound healing by reduction of oxidation and inflammation.
In recent years, many herbal topical formulations have been marketed worldwide to prevent hair loss or promote hair growth. Hair loss is not a serious disorder but often causes mental stress and affects both men and women, especially at the age of 50 years and up. The results of in vivo hair growth tests strongly suggest that C. caeruleus promoted induction of the anagen phase in the dorsal skin of rats. The results of the chromatographic analysis show that C. caeruleus contains MEHP. This biomolecule significantly induced mRNA expression of prostaglandin-endoperoxide synthase 2, an enzyme important for synthesis of prostaglandins with PGE2 mediated vasodilation and increased microvascular permeability. The C. caeruleus ointments probably improve hair growth activity by increasing the distribution of the sebaceous glands, capillaries around the hair follicles, and vasodilation to assist in forming the necessary environment for hair growth, thereby promoting hair growth by stimulating hair follicles. These findings may lead to the development of new treatments for hair loss patients. The results of the present investigation also complement the ethnobotanical usage of the studied plant which possesses several phytoconstituents with multiple biological activities.
Based on the present investigation, it is concluded that C. caeruleus L. is a potential source of bioactive compounds with great pharmaceutical value. These results confirm that C. caeruleus is a rich medicinal plant with many active molecules responsible for the anti-inflammatory, wound-healing, and hair growth properties observed by the local population of the north of Algeria. The study can be extended for the purification and isolation, in vitro and in vivo evaluation of bioactive compounds of novel drug discovery programs.
We thank deeply the Director and the Engineers of the “Research and Development Center of ALDAR Company of Moubydal Group, Algiers.”
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
| » References|| |
Doshi GM, Nalawade VV, Mukadam AS, Chaskar PK, Zine SP, Somani RR, et al.
Structural elucidation of chemical constituents from Benincasa hispida
seeds and Carissa
congesta roots by gas chromatography: Mass spectroscopy. Pharmacognosy Res 2015;7:282-93.
Oh YC, Kang OH, Kim SB, Mun SH, Park CB, Kim YG, et al.
Anti-inflammatory effect of sinomenine by inhibition of pro-inflammatory mediators in PMA plus A23187-stimulated HMC-1 cells. Eur Rev Med Pharmacol Sci 2012;16:1184-91.
Carleton BC, Smith A. Drug safety: Side effects and mistakes or adverse reactions and deadly errors? BC Med J 2006;48:329-33.
Park PJ, Moon BS, Lee SH, Kim SN, Kim AR, Kim HJ, et al.
Hair growth-promoting effect of Aconiti ciliare
Tuber extract mediated by the activation of Wnt/β-catenin signaling. Life Sci 2012;91:935-43.
Teerakul A, Sawang P, Arthid T, Atirada B, Sittiruk R. Effects of Carthamus tinctorius
L. solvent extracts on anti-proliferation of human colon cancer (SW 620 cell line) via apoptosis and the growth promotion of lymphocytes. Songklanakarin J Sci Technol 2012;34:45-51.
Attama A, Uzor P, Nnadi C, Okafor C. Evaluation of the wound healing activity of gel formulations of leaf extract of Aspilia africana
fam. (Compositae). J Chem Pharm Res 2011;3:718-24.
Meier K, Nanney LB. Emerging new drugs for wound repair. Expert Opin Emerg Drugs 2006;11:23-37.
Deters AM, Lengsfeld C, Hensel A. Oligo- and polysaccharides exhibit a structure-dependent bioactivity on human keratinocytes in vitro
. J Ethnopharmacol 2005;102:391-9.
Junlatat J, Sripanidkulchai B. Hair growth-promoting effect of Carthamus tinctorius
floret extract. Phytother Res 2014;28:1030-6.
Yadav RN, Agarwala M. Phytochemical analysis of some medicinal plants. J Phytol 2011;3:10-4.
Sawadogo WR, Lompo M, Somé N, Guissou IP, Nacoulma-Ouedraogo OG. Anti-inflammatory, analgesic and antipyretic affects of Lepidagathis anobrya
nees (ACANTHACEAE). Afr J Tradit Complement Altern Med 2001;8:420-4.
Süleyman H, Demirezer LO, Kuruüzüm A, Banoğlu ZN, Göçer F, Ozbakir G, et al.
Antiinflammatory effect of the aqueous extract from Rumex patientia
L. Roots. J Ethnopharmacol 1999;65:141-8.
Süntar I, Küpeli Akkol E, Keles H, Yesilada E, Sarker SD. Exploration of the wound healing potential of Helichrysum graveolens
(Bieb.) sweet: Isolation of apigenin as an active component. J Ethnopharmacol 2013;149:103-10.
Lodhi S, Jain AP, Rai G, Yadav AK. Preliminary investigation for wound healing and anti-inflammatory effects of Bambusa vulgaris
leaves in rats. J Ayurveda Integr Med 2016;7:14-22.
Kim SC, Kang JI, Kim MK, Hyun JH, Boo HJ, Park DB. Promotion effect of norgalanthamine, a component of Crinum asiaticum
, on hair growth. Eur J Dermatol 2010;20:42-8.
Suman S. Phytochemical investigation of Sonchus oleraceus
leaves and Citrullus colocynth
root. J Herbal Med Toxicol 2010;4:159-62.
Pires JM, Mendes FR, Negri G, Duarte-Almeida JM, Carlini EA. Antinociceptive peripheral effect of Achillea millefolium
L. and Artemisia vulgaris
L.: Both plants known popularly by brand names of analgesic drugs. Phytother Res 2009;23:212-9.
Srivastava S, Singh P, Jha KK, Mishra G, Srivastava S, Khosa RL. Antiinflammatory, analgesic and antipyretic activities of aerial parts of Costus speciosus
koen. Indian J Pharm Sci 2013;75:83-8.
] [Full text]
Siraj MA, Shilpi JA, Hossain MG, Uddin SJ, Islam MK, Jahan IA, et al.
Anti-inflammatory and antioxidant activity of Acalypha hispida
leaf and analysis of its major bioactive polyphenols by HPLC. Adv Pharm Bull 2016;6:275-83.
Aparna V, Dileep KV, Mandal PK, Karthe P, Sadasivan C, Haridas M, et al.
Anti-inflammatory property of n-hexadecanoic acid: Structural evidence and kinetic assessment. Chem Biol Drug Des 2012;80:434-9.
Xu Q, Li YH, Lü XY. Investigation on influencing factors of 5-HMF content in schisandra
. J Zhejiang Univ Sci B 2007;8:439-45.
Marzocco S, Di Paola R, Serraino I, Sorrentino R, Meli R, Mattaceraso G, et al.
Effect of methylguanidine in carrageenan-induced acute inflammation in the rats. Eur J Pharmacol 2004;484:341-50.
Saffidine K, Sahli F, Zerroug MM. Antimicrobial activity of an Algerian medicinal plant: Carthamus caeruleus
L. Phcog Community 2013;3:71-5.
Asilbekova DT, Glushenkova AI, Khushbaktova ZA, Syrov VN, Abdullaev ND. Composition of lipids from Peganum harmala
and Thermopsis alterniflora
processing wastes. Chem Natl Compd 2010;46:285-6.
Tetz LM, Aronoff DM, Loch-Caruso R. Mono-ethylhexyl phthalate stimulates prostaglandin secretion in human placental macrophages and THP-1 cells. Reprod Biol Endocrinol 2015;13:56.
[Figure 1], [Figure 2], [Figure 3], [Figure 4]
[Table 1], [Table 2], [Table 3]