IPSIndian Journal of Pharmacology
Home  IPS  Feedback Subscribe Top cited articles Login 
Users Online : 2454 
Small font sizeDefault font sizeIncrease font size
Navigate Here
 »   Next article
 »   Previous article
 »   Table of Contents

Resource Links
 »   Similar in PUBMED
 »  Search Pubmed for
 »  Search in Google Scholar for
 »Related articles
 »   Citation Manager
 »   Access Statistics
 »   Reader Comments
 »   Email Alert *
 »   Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed2788    
    Printed40    
    Emailed0    
    PDF Downloaded107    
    Comments [Add]    

Recommend this journal

 

 RESEARCH ARTICLE
Year : 2016  |  Volume : 48  |  Issue : 5  |  Page : 550-554

A simple colorimetric method for estimation of tramadol hydrochloride in pure and tablet dosage forms


1 Department of Pharmacology, Government Medical College, Thiruvananthapuram, Kerala, India
2 Department of Pharmacology, Government Medical College, Kottayam, Kerala, India

Correspondence Address:
Dr. Scaria P Thomas
Department of Pharmacology, Government Medical College, Thiruvananthapuram, Kerala
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0253-7613.190746

Rights and Permissions

Objective: The objective of this study was to develop and validate a simple method for estimation of tramadol hydrochloride (TH) in pure and pharmaceutical dosage forms using a colorimeter. Materials and Methods: TH on reaction with Eriochrome Black T in the presence of acetate buffer at pH 3.5 forms a colored complex. This complex was extracted with a fixed volume of chloroform. The optical density of this colored complex was measured against reagent blank using a colorimeter at 520 nm. Results: Beer's law was obeyed with a good correlation coefficient (0.999) in the concentration range of 2.5 μg/ml to 10 μg/ml. Drug content estimation and recovery studies carried out on commercial tablet dosage forms demonstrated the accuracy of the method and that excipients do not cause interference. Precision and robustness were measured and found to be acceptable (% relative standard deviation <2%). Conclusion: The proposed method can be used for the rapid determination of TH content in tablets at a health-care provider level using already available staff and equipment.






[FULL TEXT] [PDF]*


        
Print this article     Email this article

Site Map | Home | Contact Us | Feedback | Copyright and Disclaimer
Online since 20th July '04
Published by Wolters Kluwer - Medknow