| SHORT COMMUNICATION
|Year : 2015 | Volume
| Issue : 3 | Page : 322-324
Dimethyl sulfoxide inactivates the anticancer effect of cisplatin against human myelogenous leukemia cell lines in in vitro assays
Rahul Raghavan, Sanith Cheriyamundath, Joseph Madassery
Department of Biotechnology, University of Calicut, Malappuram, Kerala, India
Objectives: To investigate the effect of DMSO on cisplatin induced cytotoxicity (invitro) against K562 (Human mylogenous leukemia) cell line and to study the cisplatin-DMSO adduct formation using UV-spectrophotometer.
Materials and methods: Effect of DMSO on the cytotoxicity of cisplatin was studied in K562 (Chronic mylogenous leukemia) cell line by MTT assay. Cisplatin-DMSO adduct formation was studied by continuously monitoring the increase in absorption peaks for 30 minutes using UV-spectrophotometer.
Results: 0.1-0.3% DMSO markedly reduced the cytotoxic activity of cisplatin in K562 cells. Cisplatin-DMSO adduct formation was detected using UV-spectrophotometer. Continuous increase in UV absorbance between 250nm-290nm was observed when cisplatin (0.5mg/ml) and DMSO (10%) were mixed.
Conclusion: Present study revealed that DMSO inactivates the cytotoxicity of cisplatin. Cisplatin-DMSO mixture showed increased absorbance at 250-290nm.Therefore, using DMSO in invitro assays might result in misinterpretation of actual efficacy of drugs.
Prof. Joseph Madassery
Department of Biotechnology, University of Calicut, Malappuram, Kerala
Source of Support: None, Conflict of Interest: None
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