IPSIndian Journal of Pharmacology
Home  IPS  Feedback Subscribe Top cited articles Login 
Users Online : 3117 
Small font sizeDefault font sizeIncrease font size
Navigate Here
 »   Next article
 »   Previous article
 »   Table of Contents

Resource Links
 »   Similar in PUBMED
 »  Search Pubmed for
 »  Search in Google Scholar for
 »Related articles
 »   Citation Manager
 »   Access Statistics
 »   Reader Comments
 »   Email Alert *
 »   Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed1570    
    Printed29    
    Emailed2    
    PDF Downloaded104    
    Comments [Add]    

Recommend this journal

 

 SHORT COMMUNICATION
Year : 2015  |  Volume : 47  |  Issue : 1  |  Page : 117-120

Effect of adiponectin on expression of vascular endothelial growth factor and pigment epithelium-derived factor: An in vitro study


1 Research Scholar, BITS, Pilani, Rajasthan, India
2 Department of Biochemistry and Cell biology, Vision Research Foundation, Nungambakkam, Chennai, Tamil Nadu, India

Correspondence Address:
Dr. K N Sulochana
Department of Biochemistry and Cell biology, Vision Research Foundation, Nungambakkam, Chennai, Tamil Nadu
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0253-7613.150376

Rights and Permissions

Aim: This study was carried out to identify the role of adiponectin (APN) in modulating the expression of vascular endothelial growth factor (VEGF) and pigment epithelial-derived factor (PEDF) in relation to ocular angiogenesis. Materials and Methods: Human retinal pigment epithelial cell lines (ARPE-19) were cultured in Dulbeco's minimum essential medium with 10% fetal bovine serum (FBS) and exposed to varying concentrations of recombinant adiponectin (5-5 ng/ml) for 1 h. Analysis of VEGF and PEDF mRNA was done by reverse transcriptase and further quantified by quantitative polymerase chain reaction. VEGF and PEDF protein expression were studied using enzyme-linked immune sorbent assay (ELISA). Statistical Analysis: Unpaired Student's t-test was used to analyze the significance. P < 0.05 was accepted as statistically significant. Results: ARPE cells exposed to APN showed decreased expression of VEGF mRNA, protein whereas PEDF protein is unaltered and PEDF mRNA was increased. Conclusion: Our in vitro study on ARPE exposed to APN showed a negative correlation with VEGF levels. Thus indicating the protective role for APN in angiogenesis-related diseases.






[FULL TEXT] [PDF]*


        
Print this article     Email this article

Site Map | Home | Contact Us | Feedback | Copyright and Disclaimer
Online since 20th July '04
Published by Wolters Kluwer - Medknow