IPSIndian Journal of Pharmacology
Home  IPS  Feedback Subscribe Top cited articles Login 
Users Online : 1008 
Small font sizeDefault font sizeIncrease font size
Navigate Here
  Search
 
  
Resource Links
   Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
   Article in PDF (605 KB)
   Citation Manager
   Access Statistics
   Reader Comments
   Email Alert *
   Add to My List *
* Registration required (free)

 
In This Article
   References
   Article Figures

 Article Access Statistics
    Viewed2470    
    Printed48    
    Emailed1    
    PDF Downloaded113    
    Comments [Add]    
    Cited by others 1    

Recommend this journal

 


 
 Table of Contents    
LETTER TO THE EDITOR
Year : 2013  |  Volume : 45  |  Issue : 2  |  Page : 202-203
 

Synergistic potentiation of anti-anxiety activity of valerian and alprazolam by liquorice


1 Department of Pharmacognosy, K. B. Institute of Pharmaceutical Education and Research, Gandhinagar, Gujarat, India
2 Department of Pharmacology, K. B. Institute of Pharmaceutical Education and Research, Gandhinagar, Gujarat, India

Date of Web Publication11-Mar-2013

Correspondence Address:
Chaitanya Bhatt
Department of Pharmacognosy, K. B. Institute of Pharmaceutical Education and Research, Gandhinagar, Gujarat
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0253-7613.108328

Rights and Permissions



How to cite this article:
Bhatt C, Kanaki N, Nayak R, Shah G. Synergistic potentiation of anti-anxiety activity of valerian and alprazolam by liquorice. Indian J Pharmacol 2013;45:202-3

How to cite this URL:
Bhatt C, Kanaki N, Nayak R, Shah G. Synergistic potentiation of anti-anxiety activity of valerian and alprazolam by liquorice. Indian J Pharmacol [serial online] 2013 [cited 2019 Sep 17];45:202-3. Available from: http://www.ijp-online.com/text.asp?2013/45/2/202/108328


Sir,

Anxiety is a common psychological and physiological state characterized by cognitive, somatic, emotional, and behavioral components, affecting 15.7 million people in US each year. [1],[2] Valerian (Valeriana officinalis L.) has been historically popular as an anxiolytic and sedative andused as self-medication for sleeping problems. [3] It has been demonstrated that (Glycyrrhizin glabra) that if magnesium salts are administered along with saponin of liquoricehypnosis occurs. [4] This study evaluates the probable role of liquoricein potentiating anti-anxiety activity of valerian and alprazolam using elevated plus mazemodel.

The dried samples of valerian and liquoricewere obtained from LVG, Gandhi Bazaar,Ahmedabad (India). The materials were authenticated using morphological, microscopical and physicochemical parameters. Alprazolam tablet (B. No: 1390, Mfg. Date: Aug.2006, Exp. Date: July 2009), Unison Pharmaceuticals Ltd., Ahmedabad was purchased from the market. All the chemicals and reagents used for the studies were of analytical grade.

The crude materials were groundto 40 meshessieve and macerated with five times volume of ethanol:Water (7:3) for 24 h. The extracts were pooled, dried under vacuum and stored in air tight glass bottles.

Adult male Swiss albino mice (20-30 g) were housed in groups in polypropylene cages (11 × 17 × 28 cm) with wood shavings as bedding, under controlled conditions of light (12 h light-dark cycle) and temperature (25 ± 2°C). The animals had free access to water and food except 1 h before and during the experiments. The animals were allowed to acclimatize to laboratory conditions for 10 days before conducting the study. The experimental protocol wasapproved by the Institute Animal Ethics Committee (IAEC)and the experiments were carried out in accordance with theguidelines of CPCSEA on animal experimentation (Approval no. KBIPER/08/107).

Liquorice and valerian extracts(25 and 50 mg/kg, suspended in distilled water with 1% sodium CMC), or alprazolam (0.7 and 1.4 mg/kg; suspended in distilled water with 1% sodium CMC) were administered orally to mice by gavage. All the doses were prepared immediately before use. Control group was administered with the corresponding vehicle (1% sodium CMC in distilled water).

The plus-maze for mice consisted of two perpendicular open arms (30 × 5 cm) and two perpendicular closed arms (30 × 5 × 25 cm). The maze was 45 cm above the floor. One hour after oral treatment, the mouse were placed at the center of the plus-maze facing one closed arm and was observed for 20 min for number of entries in the open and closed arms and time spent in the open arms. A mouse was considered to have entered an arm when all four legs were on the arm. The percentage of time spent and percentage of entries on the open arms was considered as the anxiety index. [5]

Data obtained from elevated plus maze test were statistically analyzed using oneway ANOVA, followed by Dunnett test for comparison between various treatment groups. P < 0.05 was considered as a significant level. All data are expressed as mean ± S.D. (n = 6).

After oral administration of valerian extract to mice at 50 mg/kg and 25 mg/kg b.w., the time spent in the open arms and the percentage of number of entries in the open arms were significantly increased as compared to the vehicle-treated mice (P < 0.5). The Liquorice extract, when administered to mice at 25 and 50 mg/kg b.w., did not produce any significant behavioral change in the mice as compared to the vehicle-treated mice. Alprazolam, when administered at 1.4 mg/kg and 0.7 mg/kg b.w. to mice, showed a very significant increase (P < 0.5) in the percentage of number of entries in the open arms and time spent in the open arms [Figure 1].
Figure 1: Effect of Liquorice extract on anti-anxiety activity of Valerian extract and alprazolam in the elevated plus maze model (a) Time spent in open arms, (b) Time spent in closed arms, (c) number of entries in open arms, (d) number of entries in close arms. Data are presented as mean ±S.D. from a group of six mice. Doses are expressed as mg/ kg b.w. Val - Valerian extract, Gly - Liquorice extract, Alp - Alprazolam

Click here to view


The valerian extract (25 mg/kg b.w.), when administered orally to mice along with 25 mg/kg b.w. of liquorice extract, produced a significant increase in the percentage number of entries in the open arms andtime spent in open armsas compared to effects of valerian extract administered alone.Similar results were obtained when alprazolam was administered at 0.7 mg/kg b.w. along with liquorices extract (25 mg/kg b.w.). Thus, liquorice extract, at a therapeutically inactive dose, was found to potentiate the anti-anxiety activity of valerian and alprazolam.

The results obtained in the present study support the hypothesis that liquorice potentiates the activity of anti-anxiety drugs. The mechanism of potentiation of anti-anxiety activity by liquorice needs to be elucidated. The possible mechanism of potentiation of activity could be an increase in blood-brain barrier permeability of the drug by the constituents of liquorice, or an increase in the bioavailability of the drug due to increased absorption through gastro-intestinal tract or competitive inhibition of the metabolizing enzymes by the constituents of liquorice.

 
  References Top

1.Steingard RJ, DeMaso DR, Goldman SJ, Shorrock KL, Bucci JP. Current perspectives on the pharmacotherapy of depressive disorders in children and adolescents. Harv Rev Psychiatry 1995;2:313-26.  Back to cited text no. 1
[PUBMED]    
2.Blazer DG, Kessler RC, Mc Gonagle KA, Swartz MS. The prevalence and distribution of major depression in national community sample: The National Comorbidity Survey. Am J Psychiatry 1994;151:979-86.  Back to cited text no. 2
    
3.Diaper A, Hindmarch I. A double-blind, placebo-controlled investigation of the effects of two doses of a valerian preparation on the sleep, cognitive and psychomotor function of sleep-disturbed older adults. Phytother Res 2004;18:831-6.   Back to cited text no. 3
[PUBMED]    
4.Shah CS, Quadry JS. Textbook of Pharmacognosy. 15 th ed. Ahmedabad: B. S. Shah Prakashan; 2009. p. 264.  Back to cited text no. 4
    
5.Pellow S, Chopin P, File SE, Briley M. Validation of open: Closed arm entries in an elevated plus-maze as a measure of anxiety in the rat. J Neurosci Methods 1985;14:149-67.  Back to cited text no. 5
[PUBMED]    


    Figures

  [Figure 1]

This article has been cited by
1 Valerian: No Evidence for Clinically Relevant Interactions
Olaf Kelber,Karen Nieber,Karin Kraft
Evidence-Based Complementary and Alternative Medicine. 2014; 2014: 1
[Pubmed] | [DOI]



 

Top
Print this article  Email this article
 

    

Site Map | Home | Contact Us | Feedback | Copyright and Disclaimer
Online since 20th July '04
Published by Wolters Kluwer - Medknow