IPSIndian Journal of Pharmacology
Home  IPS  Feedback Subscribe Top cited articles Login 
Users Online : 2322 
Small font sizeDefault font sizeIncrease font size
Navigate Here
 »   Next article
 »   Previous article
 »   Table of Contents

Resource Links
 »   Similar in PUBMED
 »  Search Pubmed for
 »  Search in Google Scholar for
 »Related articles
 »   Citation Manager
 »   Access Statistics
 »   Reader Comments
 »   Email Alert *
 »   Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed2484    
    Printed74    
    Emailed1    
    PDF Downloaded110    
    Comments [Add]    
    Cited by others 2    

Recommend this journal

 

 RESEARCH ARTICLE
Year : 2012  |  Volume : 44  |  Issue : 5  |  Page : 588-592

Betulinic acid inhibits superoxide anion-mediated impairment of endothelium-dependent relaxation in rat aortas


1 Clinical Research Center,The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China
2 Clinical Research Center,The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou; Institute of Physiological Function, Medical College of Jiaxing University, Jiaxing, China
3 Institute of Physiological Function, Medical College of Jiaxing University, Jiaxing, China

Correspondence Address:
Man-Li Xia
Institute of Physiological Function, Medical College of Jiaxing University, Jiaxing
China
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0253-7613.100382

Rights and Permissions

Objectives: To investigate the protective effect of betulinic acid (BA) on endothelium-dependent relaxation (EDR) in rat aortas exposed to pyrogallol-produced superoxide anion and its underlying mechanism. Materials and Methods: The thoracic aorta of male Sprague-Dawley rats was isolated to mount in the organ bath system and the effect of BA on acetylcholine (ACh)-induced EDR, nitric oxide (NO) level, reactive oxygen species (ROS) level, nitric oxide synthase (NOS) activity, and superoxide dismutase (SOD) activity of aortic rings exposed to pyrogallol (500 μM) for 15 min were measured. Results: BA evoked a concentration-dependent EDR in aortas, and pretreatment with EC 50 (2.0 μM) concentration of BA markedly enhanced ACh-induced EDR of aortas exposed to pyrogallol-produced superoxide anion (E max rose from 23.91 ± 5.41% to 42.45 ± 9.99%), which was markedly reversed by both N w -nitro-L-arginine methyl ester hydrochloride (L-NAME) and methylene blue, but not by indomethacin. Moreover, BA significantly inhibited the increase of ROS level, as well as the decrease of NO level, the endothelial NOS (eNOS) activity, and the SOD activity in aortas induced by pyrogallol-derived superoxide anion. Conclusion: These results indicate that BA reduces the impairment of EDR in rat aortas exposed to exogenous superoxide anion, which may closely relate to the reduction of oxidative stress and activation of eNOS-NO pathway.






[FULL TEXT] [PDF]*


        
Print this article     Email this article

Site Map | Home | Contact Us | Feedback | Copyright and Disclaimer
Online since 20th July '04
Published by Wolters Kluwer - Medknow