IPSIndian Journal of Pharmacology
Home  IPS  Feedback Subscribe Top cited articles Login 
Users Online : 2330 
Small font sizeDefault font sizeIncrease font size
Navigate Here
  Search
 
  
Resource Links
   Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
   Article in PDF (440 KB)
   Citation Manager
   Access Statistics
   Reader Comments
   Email Alert *
   Add to My List *
* Registration required (free)

 
In This Article
   References
   Article Figures

 Article Access Statistics
    Viewed1490    
    Printed50    
    Emailed2    
    PDF Downloaded94    
    Comments [Add]    

Recommend this journal

 


 
 Table of Contents    
LETTER TO THE EDITOR
Year : 2011  |  Volume : 43  |  Issue : 5  |  Page : 616-617
 

In vitro antigiardial and antirotaviral activity of Psidium guajava L. leaves


1 The Foundation for Medical Research, Mumbai, India
2 Naoroji Godrej Centre for Plant Research, Lawkin Ltd. Campus, Satara, Maharashtra, India

Date of Web Publication15-Sep-2011

Correspondence Address:
Tannaz J Birdi
The Foundation for Medical Research, Mumbai
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0253-7613.84990

Rights and Permissions



How to cite this article:
Birdi TJ, Daswani PG, Brijesh S, Tetali P. In vitro antigiardial and antirotaviral activity of Psidium guajava L. leaves. Indian J Pharmacol 2011;43:616-7

How to cite this URL:
Birdi TJ, Daswani PG, Brijesh S, Tetali P. In vitro antigiardial and antirotaviral activity of Psidium guajava L. leaves. Indian J Pharmacol [serial online] 2011 [cited 2019 Sep 21];43:616-7. Available from: http://www.ijp-online.com/text.asp?2011/43/5/616/84990


Sir,

Psidium guajava L., family Myrtaceae, is used widely in traditional medicine throughout the world for a number of gastrointestinal ailments. [1] The antidiarrhoeal activity of the aqueous extract of the leaves has been studied and various mechanisms of action, namely antimicrobial activity, antispasmodic activity, inhibition of acetylcholine release, inhibition of bacterial colonization, and production/action of bacterial enterotoxins have been proposed. [1,2] The antigiardial and antirotaviral studies with P. guajava leaves from different geographical locations have been previously reported. [1] Because of known differences in the biological efficacy of medicinal plants due to differences in geographical locations we screened an Indian variety of P. guajava leaves for its in vitro antigiardial and antirotaviral activity.

Leaves of P. guajava variety Sardar collected from Parinche valley, Maharashtra, India were used. A hot aqueous extract (decoction) was used for the assays based on its field use as revealed during an ethnobotanical survey carried out by us. [3] The decoction was prepared by boiling 1 g of shade dried leaves in 16 mL double distilled water until the volume became 4 mL. [4] To replicate field conditions a fresh decoction was prepared every time. The decoction was centrifuged and filtered through a membrane of 0.22 mm pore size before use. The percent yield with respect to the starting dried plant material was 10.8% ± 0.5% (w/w); the dry weight was (27.0 ± 1.25 mg/mL) (n = 25). For the biological assays a 0.1% (0.027 ± 0.001 mg/mL), 1%, (0.270 ± 0.013 mg/ml), 5% (1.350 ± 0.063 mg/mL) and 10% dilutions (2.7 ± 0.125 mg/mL) prepared in appropriate media were used for each experiment.

For the antigiardial assay, Giardia lamblia P1 trophozoites (kindly provided by Dr. P. Das, National Institute for Cholera and Enteric Diseases, Kolkata, India) were incubated in the absence (control) and presence of different dilutions of the decoction in Diamond's TYI-SS medium. After 24 h, viability was estimated using trypan blue stain. Three independent experiments were carried out with duplicate tubes for control and each dilution of the decoction. Metronidazole (10 μg/mL) was used as the antigiardial control.

The entry and subsequent survival of simian rotavirus SA-11 (kindly provided by Dr. S. Kelkar, National institute of Virology, Pune, India) in MA-104 cells was assayed by the neutral red uptake assay. [4] Three independent experiments were carried out with triplicate wells for control as well as each dilution of the decoction.

The results are expressed as the mean ± standard error of the percent values from three independent experiments. The percentage in each experiment was calculated using the formula (C or T)/C Χ 100, where C is the mean value of the duplicate/triplicate readings of the control group and T is mean value of the duplicate/triplicate readings of the test (dilutions of the decoction) groups. Hence, the values of test groups have been represented as percentages relative to control (100%). Data were analyzed by ANOVA and Dunnett's post-test using Prism 4.0 (GraphPad, Inc., San Diego, CA, USA). P ≤ 0.05 was considered as statistically significant.

The decoction affected the viability of G. lamblia
trophozoites at 5% and 10% concentrations [Figure 1]a. The antigiardial activity though statistically significant, was lesser than that of metronidazole. Rotavirus, following entry, causes death of MA-104 cells. The decoction decreased cell death in virus infected cells at 5% and 10% concentrations indicating that it had antirotaviral activity at these concentrations [Figure 1]b. The decoction at these concentrations was not cytotoxic to MA-104 cells (data not shown).
Figure 1: Antigiardial and antirotaviral activity of a decoction of Psidium guajava L. leaves (a) Anti-giardial activity; C: Control, trophozoites in medium alone; M: Trophozoites incubated with metronidazole (10 ƒÝg/ml). Values represent mean ? standard error (n = 3) of percentage viable trophozoites relative to control (100%); *P < 0.05. (b) Anti-rotaviral activity; C: Control, rotavirus infected MA-104 cells in medium alone. Values represent mean ? standard error (n = 3) of percentage dead MA-104 cells relative to control (100%); *P < 0.05.

Click here to view


Results of the study thus showed that the decoction of P. guajava leaves has antigiardial and antirotaviral activity. Hence, combining this data with other reported studies, [1],[2] it can be suggested that P. guajava leaves have a broad spectrum of antimicrobial action and thus could be effective in controlling diarrhoea due to a wide range of pathogens.

 
  References Top

1.Gutiérrez RM, Mitchell S, Solis RV. Psidium guajava: A review of its traditional uses, phytochemistry and pharmacology. J Ethnopharmacol 2008;117:1-27.  Back to cited text no. 1
    
2.Birdi T, Daswani P, Brijesh S, Tetali P, Natu A, Antia N. Newer insights into the mechanism of action of Psidium guajava L. leaves in infectious diarrhoea. BMC Complement Altern Med 2010;10:33.  Back to cited text no. 2
    
3.Tetali P, Waghchaure C, Daswani PG, Antia NH, Birdi TJ. Ethnobotanical survey of antidiarrhoeal plants of Parinche valley, Pune district, Maharashtra, India. J Ethnopharmacol 2009;123:229-36.  Back to cited text no. 3
    
4.Brijesh S, Daswani P, Tetali P, Antia N, Birdi T. Studies on the antidiarrhoeal activity of Aegle marmelos unripe fruit: Validating its traditional usage. BMC Complement Altern Med 2009;9:47.  Back to cited text no. 4
    


    Figures

  [Figure 1]



 

Top
Print this article  Email this article
 

    

Site Map | Home | Contact Us | Feedback | Copyright and Disclaimer
Online since 20th July '04
Published by Wolters Kluwer - Medknow