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LETTER TO EDITOR
Year : 2011  |  Volume : 43  |  Issue : 2  |  Page : 222-223
 

Asymptomatic cysticercosis in wistar albino rats: A note of caution to all biomedical researchers


1 Department of Pharmacology, Burdwan Medical College & Hospital, West Bengal, India
2 Department of Pathology, Burdwan Medical College & Hospital, West Bengal, India

Date of Web Publication6-Mar-2011

Correspondence Address:
Parama Sengupta
Department of Pharmacology, Burdwan Medical College, Burdwan, West Bengal
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0253-7613.77387

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How to cite this article:
Sengupta P, Sharma A, Mazumdar G, Tripathi SK. Asymptomatic cysticercosis in wistar albino rats: A note of caution to all biomedical researchers. Indian J Pharmacol 2011;43:222-3

How to cite this URL:
Sengupta P, Sharma A, Mazumdar G, Tripathi SK. Asymptomatic cysticercosis in wistar albino rats: A note of caution to all biomedical researchers. Indian J Pharmacol [serial online] 2011 [cited 2020 Feb 21];43:222-3. Available from: http://www.ijp-online.com/text.asp?2011/43/2/222/77387


Sir,

Rat is one of the most commonly used animals in biomedical research. It can spread various types of zoonotic diseases, most commonly, rat fever (caused by Streptobacillus moniliformis), ring worm infestation, rabies, tetanus, and  Salmonella More Details. [1] Hence it is important to have a thorough knowledge regarding the common zoonotic diseases transmitted by the animal, the common modes of transmission to humans as well as necessary precautionary methods during handling of the animals.

The researcher should not only keep in mind the possible zoonotic diseases that can affect him, but also the asymptomatic parasitic diseases common to the animals that can erroneously affect the results of the experiments.

In our setting, we conducted an experiment involving Wistar albino rats, maintained according to the Committee for the Purpose of Control and Supervision on Experiments on Animals (CPCSEA) guidelines to observe the effects of fluoxetine on rat endometrium. At the end of the study, after sacrificing the animals, one of the rats of the control group (treated only with oral 0.9% normal saline) showed cyst formation on the liver. Histopathological examination confirmed the cyst to be of cestode origin as evidenced by the presence of an outer acellular eosinophilic cuticular layer and underlying subtentacular layer along with scolex-containing hooks and suckers [Figure 1]. Some giant cell reaction along with signs of inflammation was also evident in the adjoining tissue.
Figure 1: HP examination shows outer acellular eosinophilic cuticular layer, subtentacular layer along with scolex containing hooks and suckers at 100× magnification

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Molecular techniques like PCR-linked mitochondrial DNA sequencing were not used to confirm the species of the tapeworm larva. But it is most likely due to the encysted larval form of Taenia taeniaeformis, i.e. Cysticercus fasciolaris, as it is one of the most common cestodes affecting the livers of the rats.

Sometimes it has been found to be associated with hepatic fibrosarcomas. [2],[3] Hence, animal experiments which particularly involve testing of liver functions can have erroneous results if asymptomatic rats harboring C. fasciolaris are used. Although our experimental protocol did not require measurements of liver function, it most likely did not affect the outcome.

Besides Wistar albino rats, other commonly used laboratory animals can have asymptomatic parasitic infestations that can act as a restricting factor for attainment of experimental protocol. Therefore, the biomedical researchers should be aware of the asymptomatic spontaneous lesions to correctly interpret the outcome of their experiments.

 
  References Top

1.Medhi B, Prakash A. Introduction to Experimental Pharmacology. Practical Manual of Experimental and Clinical Pharmacology. 1 st ed. New Delhi: Jaypee Brothers Medical Publisher (P) Ltd.; 2010. p. 35.  Back to cited text no. 1
    
2.Mahesh Kumar J, Reddy PL, Aparna V, Srinivas G, Nagarajan P, Venkatesan R, et al. Strobilocercus fasciolaris infection with hepatic sarcoma and gastroenteropathy in a Wistar colony. Vet Parasitol 2006;141:362-7.  Back to cited text no. 2
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3.Hanes MA, Stribling LJ. Fibrosarcomas in two rats arising from hepatic cysts of cysticercus fasciolaris. Vet Pathol 1995;32:441-4.  Back to cited text no. 3
    


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