|Year : 2010 | Volume
| Issue : 5 | Page : 283-288
Effects of Ocimum sanctum and Camellia sinensis on stress-induced anxiety and depression in male albino Rattus norvegicus
Imrana Tabassum1, Zeba N Siddiqui2, Shamim J Rizvi3
1 Department of Chemistry and Interdisciplinary Brain Research Center, J.N. Medical College, Aligarh Muslim University, Aligarh - 202 002, UP, India
2 Department of Chemistry, J.N. Medical College, Aligarh Muslim University, Aligarh - 202 002, UP, India
3 Interdisciplinary Brain Research Center, J.N. Medical College, Aligarh Muslim University, Aligarh - 202 002, UP, India
|Date of Submission||30-Apr-2010|
|Date of Decision||18-Jun-2010|
|Date of Acceptance||12-Jul-2010|
|Date of Web Publication||17-Sep-2010|
Zeba N Siddiqui
Department of Chemistry, J.N. Medical College, Aligarh Muslim University, Aligarh - 202 002, UP
Source of Support: None, Conflict of Interest: None
Objective : The aim of this study was to study the ameliorative effects of Ocimum sanctum and Camellia sinensis on stress-induced anxiety and depression.
Materials and Methods : The study was carried out using male albino rats (200 ± 50 g). The effect of O. sanctum and C. sinensis was evaluated for anxiety and depression using elevated plus maze (EPM) test, open field test (OFT), forced swim test (FST), and tail suspension test (TST).
Result : Restraint stress (3 h/day for six consecutive days) induced a significant reduction in both the percentage number of entries and time spent in open arms in EPM, and these changes were reversed with post-treatment of aqueous extract of O. sanctum and C. sinensis (100 mg/kg for 6 days). Restraint stress-induced (a) increased latency and (b) decreased ambulation and rearing were also reversed by O. sanctum and C. sinensis in OFT. A significant increase in immobility period was observed in FST and TST after restraint stress. O. sanctum and C. sinensis significantly reduced the immobility times of rats in FST and TST.
Conclusion : O. sanctum and C. sinensis possess anxiolytic and antidepressant activities.
Keywords: Anxiety, Camellia sinensis, depression, Ocimum sanctum, restraint stress
|How to cite this article:|
Tabassum I, Siddiqui ZN, Rizvi SJ. Effects of Ocimum sanctum and Camellia sinensis on stress-induced anxiety and depression in male albino Rattus norvegicus. Indian J Pharmacol 2010;42:283-8
|How to cite this URL:|
Tabassum I, Siddiqui ZN, Rizvi SJ. Effects of Ocimum sanctum and Camellia sinensis on stress-induced anxiety and depression in male albino Rattus norvegicus. Indian J Pharmacol [serial online] 2010 [cited 2016 Dec 3];42:283-8. Available from: http://www.ijp-online.com/text.asp?2010/42/5/283/70108
| » Introduction|| |
The influence of environmental conditions such as stress on behavioral and cognitive processes and performance in animal models of psychiatric disorders has been widely investigated.  Stress induces a variety of autonomic, visceral, immunological, and neurobehavioral responses such as anxiety, depression, anorexia, and activation of the hypothalamic-pituitary-adrenal axis resulting in elevated corticosterone levels, in animals and humans. 
Anxiety is one of the most prominent psychiatric disorders related to a common stress. The elevated plus maze (EPM) is a well-established and widely used animal model of anxiety-like behavior for rodents.  The open field test (OFT) is also used frequently. Temporally restricted stressors, such as predator stress in the form of a 5 min exposure to a cat, are potent enough to trigger a state of enhanced and persistent anxiety [Table 1] and [Table 2].
|Table 1 :Effect of O. sanctum and C. sinensis in elevated plus-maze test (EPM) |
Click here to view
Depression is an incapacitating psychiatric ailment that has been estimated to affect 21% of the world population. Chronic stress can induce depressive disorders, and animal stress models are widely used in preclinical antidepressant evaluation.  Patients with major depression have symptoms that reflect changes in brain monoamine neurotransmitters, specifically norepinephrine, serotonin, and dopamine.  The forced swim test (FST) and the tail suspension test (TST) have been developed to evaluate depression-like behaviors in mice. , The immobility of the animal in FST reflects a state of "behavioral despair." The TST also induces a state of despair in animals like that in FST [Table 3] and [Table 4].
|Table 3 :Effect of O. sanctum and C. sinensis on immobility period of R. norvegicus using tail suspension test (TST) |
Click here to view
|Table 4 :Effect of O. sanctum and C. sinensis on immobility period of R. norvegicus using forced swim test |
Click here to view
Restraint stress can induce a series of dysfunctions of central nervous system, such as cognitive impairment, anxiety, depression, amnesia, and insomnia. Therefore, close attention was paid to studies of protecting effects of natural potential ingredients on stress-induced injury. Several plants have been investigated, which were once used as tonics due to their adaptogenic and rejuvenating properties in traditional medicine. The drugs of plant origin are gaining increasing popularity and are being investigated for remedies of a number of disorders including antistress adaptogenic activity. 
Epidemiological studies have suggested positive association between the consumption of phenolic-rich foods or beverages and the prevention of diseases. These effects have been attributed to antioxidant components such as plant phenolics, flavonoids, and phenylpropanoids among others.  Ocimum. sanctum and Camellia sinensis contain an abundance of naturally occurring polyphenols. Recent studies have shown that polyphenols possess potential effects of neuroprotection.  So far, no study has appeared about the protective effects of O. sanctum and C. sinensis on anxiety and depression induced by restraint stress. Considering substantial neuroprotective properties of O. sanctum and C. sinensis, we sought to investigate whether O. sanctum and C. sinensis could improve cognitive impairments induced by restraint stress in rats; and to explore the underlying mechanisms for the same.
| » Materials and Methods|| |
Leaves of O. sanctum were collected from University campus, identified by a pharmacognist (I.D. No. Husain 1375) and deposited for the record in Aligarh Muslim University (AMU) Herbarium.
Leaves of C. sinensis were purchased from an authorized dealer, identified by a pharmacognist (I.D. No. Husain 395) and deposited in AMU, Herbarium as a record.
Extraction of O. sanctum and C. sinensis
Briefly, the shade dried powder of O. sanctum and C. sinensis was refluxed for 24 h with double-distilled water (DDW) at 100ºC, cooled, and filtered. The solvent was removed under reduced pressure to get the product. The yield of the extracts of O. sanctum and C. sinensis were 9% and 7% (w/w), respectively, in terms of dried starting material. The residue was stored in the refrigerator until further use. The dose (100 mg/kg) was selected on the basis of preliminary studies that assessed the ability of O. sanctum to attenuate the stress-induced alteration in behavioral tests. 
Adult male albino rats (200 ± 50 g) were obtained from Central Animal House facility of J.N m0 edical c0 ollege, AMU, Aligarh. The animals kept in polypropylene cages, were housed in air-conditioned room, and maintained on standard pellet diet and water ad libitum.
Thirty-six rats were used in this study, and they were divided into six groups. First group treated as control and second was of restraint stress (3 h/day for six consecutive days). Third and fourth groups were treated with the post-treatment of O. sanctum and C. sinensis (100 mg/kg) aqueous leaf extracts following restraint stress. Fifth and sixth groups were treated with O. sanctum and C. sinensis alone, respectively. The study was approved by Institutional Animals Ethics Committee (401/CPCSEA).
The animals were subjected to restraint stress in a wire mesh restrainer [Figure 1] for 3 h/day for six consecutive days. The size of restrainers could be adjusted according to the size of the rat ensuring that every rat was immobile. Immediately after the stress procedure, the rats were exposed to behavioral tests.
Elevated Plus Maze Test
This test has been proposed for selective identification of anxiolytic and anxiogenic drugs. The EPM consisted of two open arms and two enclosed arms, 50 Χ 40 Χ 40 cm 3 with an open roof, assayed so that the two arms were opposite to each other. The maze was elevated to a height of 50 cm.  After treatment with each group, the rats were placed in the center of the maze, facing one of the enclosed arms. During 5 min test period, the parameters measured were: number of open arm entries and closed arm entries. Subsequently, the percentages of open arm entries and time spent on open arms were calculated from open arm entries and time spent on open arms divided by the total number of entries in both open and closed arms and time spent on open arm exploration divided by total time spent in both open and closed arms, respectively. The procedure was conducted in sound attenuated room.
Open Field Test
Locomotor activity was quantified for 5 min in an open field. It was consisted of a square arena 96 Χ 96 cm 2 with 60 cm high walls. The walls and the floor were painted in white. The floor was divided in to 16 squares by parallel and intersecting white lines.  Four squares defined as the center and the 12 squares along the walls as the periphery. Rats were placed in the very center of the open field and (a) latency, (b) ambulation, and (c) rearing were observed during a 5 min exposure period for both control and treated animals.
Forced Swim Test
The test was performed according to a modification suggested by Lucki of the traditional method.  The apparatus consisted of a transparent cylinder (50 cm high Χ 20 cm wide) filled to 30 cm depth with water at room temperature. The water depth was adjusted so that the animals must swim or float without their hind limbs or tail touching the bottom. The duration of immobility was recorded during the last 5 min of the 6 min test swimming session. A rat was judged to be immobile when it floated in an upright position, making only small movements to keep its head above water.
Tail Suspension Test
A short piece of paper adhesive tape (about 6 cm) was attached along half the length of the tail (about 3 cm). The free end of the tape was attached to a 30 cm long rigid tape, which hung from a horizontal bar clamped to a heavy laboratory support stand. Suspended animals were surrounded by a white wooden enclosure (40 cm high, 40 cm wide, and 40 cm deep) such that the rat's head was about 20 cm above the floor. For testing, each rat was suspended by its tail and observed for 6 min. An observer scored the total duration of a passive, "dead weight" hanging (immobility), between the periods of wriggling of the animal to avoid aversive situation. 
All values are expressed as mean SEM. Behavioral data were analyzed using Student's t-test. Significance level was chosen at P < 0.001. All statistical analyses were carried out by using SPSS for Windows (SPSS 10.0).
| » Results|| |
Elevated Plus Maze Test
Analysis of EPM data revealed that restraint stress (3 h/day for six consecutive days) induced a significant reduction in percentage open arm entries by 50% and percentage time spent in open arms by 63% as compared to control (P < 0.05). Post-treatment of aqueous extracts of O. sanctum and C. sinensis (100 mg/kg for 6 days) reversed the restraint stress-induced changes in both open arm entries and percentage time spent in open arms. O. sanctum increased the percentage number of open arm entries by 54%, whereas C. sinensis by 38%, respectively, as compared to stress group (P < 0.01, P < 0.05). Percentage time spent in open arms was also increased by 65% and 53% with O. sanctum and C. sinensis [Figure 2].
|Figure 2 :Bar diagram showing the alteration of % open arm entries and % time spent in EPM following restraint stress (3h/day for 6 days) and ameliorative action of O. sanctum and C. sinensis (100 mg/kg/day for 6 days) on Rattus norvegicus.|
Click here to view
Open Field Test
Restraint stress-induced a significant increase in the latency by 48%, decrease in ambulation by 38%, and rearing by 59% as compared to control (P < 0.05). Post-administration of O. sanctum and C. sinensis attenuated the restraint stress effects on the open field behavior. Latency was decreased by 52% with O. sanctum and by 25% with C. sinensis. Ambulation was increased by 41% and 33% with O. sanctum and C. sinensis. Rearing activity was also increased by 64% and 49% after the treatment of O. sanctum and C. sinensis, respectively, as compared to stress (P < 0.01, P < 0.05) [Figure 3].
|Figure 3 :Bar diagram showing the alteration of latency, ambulation, and rearing in OFT following restraint stress (3 h/day for six consecutive days) and ameliorative action of O. sanctum and C. sinensis (100 mg/kg/day for 6 days) on R. norvegicus.|
Click here to view
Forced Swimming Test
Restraint stress significantly increased the immobility by 53% in the forced swimming test as compared to control (P < 0.001). O. sanctum caused a 57% reduction in immobility, whereas C. sinensis by 49% as compared to control (P < 0.05) [Figure 4].
|Figure 4 :Bar diagram showing the alteration of immobility time in FST following restraint stress (3 h/day for six consecutive days) and ameliorative action of O. sanctum and C. sinensis (100 mg/kg/day for 6 days) on R. norvegicus.|
Click here to view
Tail Suspension Test
Restraint stressed rats exhibited a significant increase in immobility period by 46% as compared to control animals (P < 0.001). When O. sanctum and C. sinensis were administered, significant protection was observed in the anti-immobility effects of O. sanctum and C. sinensis, i.e. O. sanctum decreased the immobility by 48% while C. sinensis by 40%, respectively, as compared to stress [Figure 5]. There was no significant change observed in the behavioral tests when O. sanctum and C. sinensis were given administered.
|Figure 5 :Bar diagram showing the alteration of immobility time in TST following restraint stress (3h/day for 6 days) and ameliorative action of O. sanctum and C. sinensis (100 mg/kg/day for 6 days) on Rattus norvegicus.|
Click here to view
| » Discussion|| |
Environmental factors like stress can influence the neurobehavioral profile of the organism and precipitate an anxiety-like syndrome. Behavioral factors such as emotionality are useful predictors of stress susceptibility.  Both EPM and OFT have been used very effectively to assess neurobehavioral profile of animals under the influence of anxiogenic/anxiolytic agents. , Our results indicated that restraint stress caused a significant reduction in the % number of entries and % time spent in open arms in EPM. Similarly in the OFT restraint stress induced behavioral alterations as evidenced by increase in latency and decrease in ambulation and rearing. These are the indications of high level of fear or anxiety. Our results are in agreement with earlier studies. ,
In the EPM increase in both percentage number of entries and percentage time spent in open arms are indices of anxiolytic activity and our results with aqueous extract of O. sanctum and C. sinensis were consistent with anti-stress effect of these medicinal plants. Similarly in the OFT, O. sanctum and C. sinensis decreased latency and increased ambulation and rearing. These behavioral changes were suggestive of decreased fear or anxiety. Thus, O. sanctum and C. sinensis showed anxiolytic effects against restraint stress. Our results were strongly supported by the study of Gopala Krishna et al. who reported anxiolytic activity of NR-ANX-C, a polyherbal preparation, containing aqueous extracts of Withania somnifera and Shilajit and alcoholic extracts of O. sanctum and C. sinensis.
O. sanctum has been shown to possess cortisol sparing immunostimulant and antioxidant activities. This cortisol sparing immunomodulatory activity of O. sanctum may also contribute to the behavioral disinhibitory activity.  Earlier studies have shown that C. sinensis extract contain many of polyphenolic antioxidants such as catechins, epicatechin, and epigallocatechin gallate. Epicatechin, one of its polyphenolic constituents, has been found to enhance learning and memory ability in mice.  Epigallocatechin gallate markedly increased protein kinase C in the membrane and the cytosolic fractions of mice hippocampus the learning site of brain.  Oral administration of the aqueous extract of M. officinalis in doses ranging from 10 to 100 mg/kg enhanced the exploratory behavior of mice in the EPM and OFT and induced sedation.  These studies further support our findings.
The antidepressant activity has been reported from medicinal plants such as Apocynum venetum and Bacopa monniera. , The results presented here show, to our knowledge for the first time, that O. sanctum and C. sinensis given orally is effective in producing significant antidepressant-like effects, when assessed in FST and in TST. Both FST and TST are widely used to screen new antidepressant drugs. , These tests are quite sensitive and relatively specific to all major classes of antidepressant drugs including tricyclics, 5-HT-specific reuptake inhibitors, MAO inhibitors, and atypicals. , The FST and TST reflecting a state of despair is reduced by several agents which are therapeutically effective in human depression. This immobility, referred to as behavioral despair in animals, is claimed to reproduce a condition similar to human depression. 
The precise mechanisms by which O. sanctum and C. sinensis produced antidepressant-like effects are not completely understood. Various antidepressant drugs, either by inhibiting MAO-enzyme or by inhibiting reuptake mechanism, increase the central monoamine levels or reverse the stress-induced depressive-like behavior.
Previous studies have shown that noise stress-induced alteration in brain monoamine neurotransmitters (norepinephrine, epinephrine, dopamine, and serotonin). Administration of O. sanctum had a normalizing action and controlled the alteration in neurotransmitter levels due to stress. , Therefore, the antidepressant activity of O. sanctum may be correlated with these studies.
Camellia sinensis in general as well as its phenolic components catechins and epigallocatechin gallate, have been found to be effective in inhibiting MAO level. Theanine, an amino acid, found in C. sinensis has also been found to have beneficial effects by raising the levels of serotonin and dopamine in various important brain regions, particularly the hypothalamus, hippocampus, and striatum.  These studies suggest that antidepressant effects might be produced by interaction with adrenoceptors, 5-HT, dopamine receptors, and monoamine oxidase, thereby increasing or decreasing the levels of noradrenaline, 5-HT, and dopamine in the brains of rats. Several herbal preparations that have antidepressant potential also possess antioxidant effects.  Thus, antioxidant action may have a role in the mechanism responsible for antidepressant activity. Since O. sanctum and C. sinensis are antioxidant medicinal plants, the antidepressant activity of O. sanctum and C. sinensis may be due to their antioxidant activity of these plants.
In conclusion, O. sanctum and C. sinensis exhibited anxiolytic and antidepressant-like effect in restraint stress-induced anxiety and depression model in rats and these effects may be mediated by the central monoaminergic neurotransmitter system (5-HT and dopamine). In the entire behavioral test, O. sanctum shows higher protection than C. sinensis. It may be concluded, therefore, that O. sanctum is more protective as compared to C. sinensis in anxiety and depression.
| » Acknowledgment|| |
The authors are grateful to the Chairman, Department of Chemistry for providing necessary research facilities and UGC for providing financial assistance to the first author (IT).
| » References|| |
|1.||Chrousos GP and Gold PW. The concepts of stress and stress system disorders. JAMA 1992;267:1244-52. |
|2.||Korte MS, Boer SF. A robust animal model of state anxiety: Fear-potentiated behavior in the elevated plus-maze. Eur J Pharmacol 2003;463:163-75. |
|3.||Garcia R. Stress, metaplasticity, and antidepressants. Curr Mol Med 2002;2:629-38. [PUBMED] [FULLTEXT] |
|4.||Gold PW, Goodwin FK, Chrousos GP. Clinical and biochemical manifestations of depression in relation to the neurobiology of stress: Part 1. N Engl J Med 1988;319:348-53. [PUBMED] [FULLTEXT] |
|5.||Porsolt RD, Bertin A, Jalfre M. Behavioral despair in mice: Primary screening test for antidepressants. Arch Int Pharmacodyn Ther 1977;229:327-36. [PUBMED] |
|6.||Steru L, Chermat R, Thierry B, Simon P. The tail suspension test: A new method for screening antidepressants in mice. Psychopharmacology 1985;85:367-70. [PUBMED] |
|7.||Edzard E. Harmless herbs? A review of the recent literature. Am J Med 1998;104:170-8. |
|8.||Rice-Evans CA, Miller NJ, Paganga G. Structure-antioxidant activity relationship of flavonoids and phenolic acids. Free Radic Biol 1996;20:933-56. |
|9.||Chen WQ, Zhao XL, Hou Y, Li ST, Hong Y, Wang DL, Cheng YY. Protective effects of green tea polyphenols on cognitive impairments induced by psychological stress in rats. Behav Brain Res 2009;202:71-6. [PUBMED] [FULLTEXT] |
|10.||Kumar SR, Rao SM, Nayak S, Sareesh NN. Effect of Ocimum sanctum (Linn) extract on restraint stress induced behavioral deficits in male wistar rats. Pharmacologyonline 2007;3:394-404. |
|11.||Montgomery KC. The relation between fear induced by novel stimulation and exploratory behavior. J Comp Physiol Psychol 1958;48:254-60. |
|12.||Bhattacharya SK, Satyan KS. Experimental methods for the evaluation of psychotropic agents. Ind J Exp Biol 1997;35:565-75. |
|13.||Henke PG, Ray A, Sullivan RM. The amygdala, emotions and gut functions. Dig Dis Sci 1991;36:1633-43. [PUBMED] |
|14.||Masood A, Banerjee B, Vijayan VK, Ray A. Modulation of stress-induced neurobehavioral changes by nitric oxide in rats. Eur J Pharmacol 2003;458:135-9. [PUBMED] [FULLTEXT] |
|15.||Chakraborti A, Gulati K, Banerjee BD, Ray A. Possible involvement of free radicals in the differential neurobehavioral responses to stress in male and female rats. Behav Brain Res 2007;179:321-5. [PUBMED] [FULLTEXT] |
|16.||Gopala Krishna HN, Sangha RB, Misra N, Pai MR. Antianxiety activity of NR-ANX-C, a polyherbal preparation in rats. Ind J Pharmacol 2006;38:330-5. |
|17.||Matsuoka Y, Hasegawa H, Okuda S, Muraki T, Uruno T, Kubota K. Ameliorative effects of tea catechins on active oxygen-related nerve cell injuries. J Pharmacol Exp Ther 1995;274:602-8. [PUBMED] [FULLTEXT] |
|18.||Levites Y, Amit T, Mandel S, Youdim MB. Neuroprotection and neurorescue against Abeta toxicity and PKC-dependent release of nonamyloidogenic soluble precursor protein by green tea polyphenol (-)-epigallocatechin-3-gallate. The FASEB J 2003;17:952-4. |
|19.||Coleta M, Campos MG, Cotrim MD, Proenca da Cunha A. Comparative evaluation of Melissa officinalis L, Tilia europaea L, Passiflora edulis Sims and Hypericum perforatum L in the elevated plus maze anxiety test. Pharmacopsychiatry 2001;34:S20-1. |
|20.||Butterweck V, Nishibe S, Sasaki T, Uchida M. Antidepressant effects of Apocynum venetum leaves in a forced swimming test. Biol Pharm Bull 2001;24:848-51. [PUBMED] [FULLTEXT] |
|21.||Sairam K, Dorababu M, Goel RK, Bhattacharya SK. Antidepressant activity of standardized extract of Bacopa monniera in experimental models of depression in rats. Phytomedicine 2002;9:207-11. [PUBMED] |
|22.||Samson J, Sheela Devi R, Ravindran R, Senthilvelan M. Biogenic amine changes in brain regions and attenuating action of Ocimum sanctum in noise exposure. Pharmacol Biochem Behav 2006;83:67-75. [PUBMED] [FULLTEXT] |
|23.||Ravindran R, Rathinasamy SD, Samson J, Senthilvelan M. Noise stress induced brain neurotransmitter changes and the effect of Ocimum sanctum (Linn) treatment in albino rats. J Pharmacol Sci 2005;98:354-60. [PUBMED] [FULLTEXT] |
|24.||Yokogoshi H, Kobayashi M, Mochizuki M, Terashima T. Effect of theanine, r-glutamylethylamide, on brain monoamines and striatal dopamine release in conscious rats. Neurochem Res 1998;23:667-73. [PUBMED] [FULLTEXT] |
|25.||Zhang JZ. Therapeutic effects of herbal extracts and constituents in animal models of psychiatric disorders. Life Sci 2004;75:1659-99. |
[Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5]
[Table 1], [Table 2], [Table 3], [Table 4]
|This article has been cited by|
||Densitometric Validation and Optimisation of Polyphenols inOcimum sanctumLinn by High Performance Thin-layer Chromatography
| ||Ilyas U.K.,Deepshikha P. Katare,Vidhu Aeri |
| ||Phytochemical Analysis. 2015; : n/a |
|[Pubmed] | [DOI]|
||Canadian boreal pulp and paper feedstocks contain neuroactive substances that interact in vitro with GABA and dopaminergic systems in the brain
| ||Andrew Waye,Malar Annal,Andrew Tang,Gabriel Picard,Frédéric Harnois,José A. Guerrero-Analco,Ammar Saleem,L. Mark Hewitt,Craig B. Milestone,Deborah L. MacLatchy,Vance L. Trudeau,John T. Arnason |
| ||Science of The Total Environment. 2014; 468-469: 315 |
|[Pubmed] | [DOI]|
||Ocimum sanctum linn (TULSI) - an overview
| ||Mohan, L., Amberkar, M.V., Kumari, M. |
| || International Journal of Pharmaceutical Sciences Review and Research. 2011; 7(1): 51-53 |
||Anxiogenic effect of lansoprazole in wistar rats
| ||Mohan, L., Shenoy, S., Ramani, A., Saravanan, Shivakumar |
| || International Journal of Pharmaceutical Sciences Review and Research. 2011; 7(1): 86-88 |