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 RESEARCH ARTICLE
Year : 2010  |  Volume : 42  |  Issue : 2  |  Page : 99-104

In vitro and in vivo hepatoprotective effects of the total alkaloid fraction of Hygrophila auriculata leaves


1 Manipal College of Pharmaceutical Sciences, Manipal University, Manipal - 576 104, India
2 J.S.S.College of Pharmacy, Manipal University, Manipal - 576 104, India

Correspondence Address:
Mallikarjuna C Rao
Manipal College of Pharmaceutical Sciences, Manipal University, Manipal - 576 104
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0253-7613.64500

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Objective: To investigate the total alkaloid fraction of the methanol extract of leaves of Hygrophila auriculata for its hepatoprotective activity against CCl4-induced toxicity in freshly isolated rat hepatocytes, HepG2 cells, and animal models. Materials and Methods: Mature leaves of H. auriculata were collected, authenticated, and subjected to methanolic extraction followed by isolation of total alkaloid fraction. Freshly isolated rat hepatocytes were exposed to CCl4 (1%) along with/without various concentrations of the total alkaloid fraction (80-40 μg/ml). Protection of human liver-derived HepG2 cells against CCl4-induced damage was determined by the MTT assay. Twenty-four healthy Wistar albino rats (150-200 g) of either sex were used for the in vivo investigations. Liver damage was induced by administration of 30% CCl4 suspended in olive oil (1 ml/kg body weight, i.p). Results: The antihepatotoxic effect of the total alkaloid fraction was observed in freshly isolated rat hepatocytes at very low concentrations (80-40 μg/ml). A dose-dependent increase in the percentage viability was observed when CCl4-exposed HepG2 cells were treated with different concentrations of the total alkaloid fraction. Its in vivo hepatoprotective effect at 80 mg/kg body weight was comparable with that of the standard Silymarin at 250 mg/kg body weight. Conclusion: The total alkaloid fraction was able to normalize the biochemical levels which were altered due to CCl4 intoxication.






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